Live-or-Dye Fixed Viability Stains are amine reactive dyes that are impermeable to cell membranes. Dyes can enter dead cells that have damaged membrane integrity ａnd covalently label free amines on intracellular proteins. Dye labeling is very stable ａnd can immobilize ａnd penetrate cells without losing fluorescence or dye transfer between cells. Live-or-Dye The staining scheme has been optimized to maximize the differentiation of live/dead cells, with minimal staining of live cells to prevent interference with immune staining.

Biotium has multiple Live or Dye options Reactive dyes are available for selection, covering the fluorescence spectrum, ａnd achieving maximum flexibility in designing multi-color flow plates.

Biotium also offers two sampler kits, each containing five different dye colors, for excitation from all popular flow cytometry laser lines. Live-or-Dye The Sampler Kit, Standard (32016) is designed for the most common flow cytometry laser ａnd filter configurations, where dyes can be excited by UV, Violet, Blue, Yellow Green, ａnd Red lasers. Live-or-Dye The Sampler Kit, Spectral (32017) is designed for spectral scanning flow cytometry. It contains dyes that can be excited by ultraviolet, violet, blue, ａnd red lasers, all of which have been demonstrated in Cytek ®  The Aurora spectral cell analyzer was validated ａnd selected due to its ability to fill many gaps in the spectral flow panel.

Live-or-Dye Fixed Viability Stains have dead cell specificity for all cell types, including mammalian cells, bacteria, ａnd yeast.

UV/448可固定活性染色试剂盒允许通过流式细胞术或显微镜来区分活细胞和死细胞。在通过流式细胞术分析细胞表面蛋白表达时，活/死染色是有用的探针，因为它们允许将来自具有可渗透质膜的死细胞的细胞内荧光信号排除在分析之外。

艾美捷UV/448可固定活性染色试剂盒（200次测定）#32002组件：

Live-or-Dye™ 可固定死细胞染料

无水二甲基亚砜

采样器套件组件

5 种不同的 Live-or-Dye™ 可固定死细胞染料

无水二甲基亚砜

UV/448可固定活性染色试剂盒特征：

高度死细胞特异性

用活性染料进行共价标记

承受固定和透化

多种颜色选择

为光谱流设计的染料选项

方便的采样器套件

UV/448可固定活性染色试剂盒，涵盖荧光光谱，在多色分析中实现非常大的灵活性。

来源：https://www.amyjet.com/products/BTM-32002.shtml