Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion ａnd two antigen binding Fab portions joined together by disulfide bonds ａnd therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures ａnd is the most cost effective.

Usage

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.

Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Preservative: None

Suggested Working Concentration or Dilution Range:

10-20 µg / ml

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

基于免疫电泳和/或ELISA抗体，抗体与人IgA的重链反应，但不与人IgG或IgM反应。未检测到针对非免疫球蛋白血清蛋白的抗体。该抗体可能与其他物种的IgA发生交叉反应。

通过免疫亲和色谱法从抗血清中分离出完整的 IgG 抗体作为完整分子。它们有一个Fc部分和两个抗原结合Fab部分通过二硫键连接在一起，因此它们是二价的。据报道，平均分子量约为160kDa。整个IgG形式的抗体适用于大多数免疫检测程序，并且非常具有成本效益。

艾美捷AffiniPure兔抗人血清伊加，α链特异性#309-005-011用法：

物理状态：无菌过滤液

存储： 在无菌条件下储存在2-8°C。使用当天准备工作稀释液。

有效期：自收到之日起一年。如果测试结果可以接受预期用途，则有效期可以延长。

纯度：使用与琼脂糖珠偶联的抗原通过免疫亲和色谱法从抗血清中纯化抗体。

缓冲液：0.01M磷酸钠，0.25M氯化钠，pH 7.6

防腐剂：无

建议工作浓度或稀释范围：

10-20μg/ ml

稀释因子以范围的形式呈现，因为佳稀释度是许多因素的函数，例如抗原密度，渗透性等。实际使用的稀释度必须根据经验确定。

AffiniPure兔抗人血清伊加，α链特异性#309-005-011

目标： 人类

宿主：兔

抗体形式：全 IgG 特异性：血清 IgA，α链特异性

偶联物：非偶联

产品类别：全 IgG

亲和纯化抗体

克隆性：多克隆

RRID：AB_2339627

来源;https://www.amyjet.com/products/309-005-011.shtml