产品名称：MEGAscript™ T7 Transcription Kit

MEGAscript™ T7 Transcription Kit现货促销价格产品资料：

The MEGAscript® T7 Kit is an ultra-high yield in vitro transcription kit. The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used. The T7 Enzyme Mix ａnd the 10X Reaction Buffer are specifically calibrated for each lot ａnd RNA polymerase.

Using the MEGAscript® T7 Kit
The MEGAscript® T7 Kit contains in vitro transcription reaction components ａnd a control template. The kit will yield a total of 3–5 mg of RNA (approximately 100 µg of RNA or more per reaction) from the control template supplied with the kit. This corresponds to 400–650 moles of RNA for each mole of template. Smaller templates typically yield a lower mass ａnd a higher molar yield of product. Novel transcription reaction conditions ａnd a patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions.

Applications
The MEGAscript® T7 Kit is intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, ａnd isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all 4 nucleotides is high, well above the K_m for the enzyme. The MEGAscript® T7 Kit typically yields over 10 times more RNA than conventional in vitro transcription reactions. The kit is not recommended for synthesis of high specific activity probes.

Accessory products
MessageAmp™ aRNA amplification kits are powered by MEGAscript® technology ａnd have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitrotranscription products from free nucleotides, buffer components, ａnd enzymes, we recommend using the MEGAclear™ Kit (Cat. No. AM1908) ａnd the MEGAclear™-96 Kit (Cat. No. AM1909).

MEGAscript™ T7 Transcription Kit现货促销价格参数规格

产品资料

T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, ａnd Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.