无创管

无细胞血浆DNA和细胞基因组DNA的提取

 

无细胞血浆DNA和细胞基因组DNA的提取可通过大部分市售试剂盒完成。

如欲获取zui佳结果，在60℃离液盐中加入一个蛋白酶K处理步骤（≥ 30 mAU/ml），提取无细胞DNA需1小时，提取细胞基因组DNA需2小时。

 

注：

1. Cell-Free DNA BCT STRECK无创管不会稀释血液， 因此，无需进行稀释因数校正来

2. 溶血、黄疸和脂血均能影响STRECK无创管中保存血样所得的检测结果。

3. 未使用的试管应保存在18-30℃之间。

4. 抽取进其他抗凝血剂或防腐剂中的样本，在STRECK无创管中可能会导致凝结。

 

Cell-Free DNA BCT STRECK无创管规格

 

抽血量             10.0ml

抗凝血剂           K3EDTA

添加剂             专有稳定剂

使用前储存         室温保存

 

 

参考文献

 

 

2. Das K., Fernando M.R., Basiaga S., Wigginton S., Williams T. Effects of a novel cell stabilizing reagent on DNA amplification by PCR as compared to traditional stabilizing reagents. Acta Histochemica 2014; 116:55-60.

3. Norton S.E., Lechner J.M., Williams T., Fernando, M.R. A stabilizing reagent prevents cell-free DNA contamination by cellular DNA in plasma during blood sample storage ａnd shipping as determined by digital PCR. Clinical Biochemistry 2013; 46: 1561-1565.

4. Norton S.E., Luna K.K., Lechner J.M., Qin J., Fernando M.R. A new blood collection device minimizes cellular DNA release during sample storage & shipping when compared to a standard device.

5. Qin J., Alt J.R., Hunsley B., Williams T., Fernando M.R. Stabilization of circulating tumor cells in blood using a collection device with a preservative reagent. Cancer Cell International 2014, 14:23.

6. Clinical ａnd Laboratory Standards Institute. H3-A6, Procedures for the collection of diagnostic blood specimens by venipuncture; approved standard-sixth edition.

详情点击：北京群晓科苑生物技术有限公司

http://www.chem17.com/st289923/product_22167865.html 

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