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Rabbit Anti-Complement C3 , FITC conjugated
阅读次数:314发布时间:2015/6/1 15:12:00
Catalog Number : RSF-02934R-FITC
Quantity size : 100ug (2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and 0.1% Sodium azide. Reconstitute with 100ul sterile distilled water. )
background:
The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.
Specificity : · anti-Complement C3 is a rabbit Polyclonal antibody, FITC conjugated
· specific for anti-Complement C3/FITC of Human, Mouse, Rat
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt:181 kDa
Application :
· FCM: 1:50-200
·IF : 1:50-1:200
· Immunocytochemistry: 1:50-1:200
· Excitation spectrum: 490-495 nm
· Emission spectrum: 520-530 nm
· Optimal working dilutions must be determined by the end user.
· protect from light.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.