产品介绍
美国ABI 平端克隆试剂盒K275020
价 格：￥电议
型 号：K275020
产品完善度：
生产地：其他访问量：114次
发布日期：2015/7/17 11:26:32
更新日期：2023/10/7 10:38:48
详细内容
The Zero Blunt® PCR Cloning Kit offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proof-reading, thermostable DNA polymerases. The Zero Blunt® PCR Cloning Kit uses the multipurpose cloning vector pCR-Blunt ａnd ExpressLink? T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step.
Features of the Zero Blunt® Cloning® Kit with pCR-Blunt vector:
Fast & convenient—5-minute, room-temperature ligation
Efficient—ccdB gene for positive selection results in >80% clones with correct insert
Flexible—choice of kanamycin or Zeocin resistance for flexible antibiotic selection
The pCR?-Blunt vector provides:
EcoR I sites flanking the PCR product insertion site for excision of inserts
T7 promoter/primer site for in vitro RNA transcription ａnd sequencing
M13 forward ａnd reverse primer sites for sequencing or PCR screening
How Zero Blunt® PCR Cloning Works
The Zero Blunt® PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants. The pCR-Blunt vector contains the lethal E. coliccdB gene fused to the C-terminus of LacZα (Bernard et al., 1994). Ligation of a blunt PCR fragment disrupts expression of the lacZα-ccdB gene fusion permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed when the transformation mixture is plated.
Kit Configurations
The Zero Blunt® PCR Cloning Kit is offered in a variety of configurations: with One Shot® TOPO10 Chemically Competent E. coli (K2700-20 ａnd K2700-40) ａnd without competent cells (K2750-20 ａnd K2750-40) in 20- ａnd 40- reaction kit sizes.
Fast & convenient—5-minute, room-temperature ligation
Efficient—ccdB gene for positive selection results in >80% clones with correct insert
Flexible—choice of kanamycin or Zeocin resistance for flexible antibiotic selection
The pCR?-Blunt vector provides:
EcoR I sites flanking the PCR product insertion site for excision of inserts
T7 promoter/primer site for in vitro RNA transcription ａnd sequencing
M13 forward ａnd reverse primer sites for sequencing or PCR screening
How Zero Blunt® PCR Cloning Works
The Zero Blunt® PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants. The pCR-Blunt vector contains the lethal E. coliccdB gene fused to the C-terminus of LacZα (Bernard et al., 1994). Ligation of a blunt PCR fragment disrupts expression of the lacZα-ccdB gene fusion permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed when the transformation mixture is plated.
Kit Configurations
The Zero Blunt® PCR Cloning Kit is offered in a variety of configurations: with One Shot® TOPO10 Chemically Competent E. coli (K2700-20 ａnd K2700-40) ａnd without competent cells (K2750-20 ａnd K2750-40) in 20- ａnd 40- reaction kit sizes.
规格
| Vector Type: | Blunt DNA Cloning Vectors |
|---|---|
| Cloning Method: | Blunt PCR |
| Bacterial or Yeast Strain: | Not Included |
| Product Size: | 20 reactions |
内容及储存
Zero Blunt® PCR cloning kits contain linearized pCR-Blunt vector, ExpressLink T4 DNA ligase , 5X ExpressLink T4 DNA ligase buffer, control template, dNTPs, sterile water, ａnd M13 forward ａnd reverse primers.
Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
| K275020 | Zero Blunt® PCR 平端克隆试剂盒（不含感受态） | 3070 |
| K202020 | TA 克隆试剂盒（pCR?2.1 Vector，不含感受态） | 3390 |
