1.购买我公司任意ELISA试剂盒指标享受免费代测，并提供技术指导服务！

二、实验材料与试剂配制：

1. 仪器与材料：酶标仪（使用前预热30分钟），微量加液器、吸头、蒸馏水或去离子水，滤纸。

2. 缓冲液使用：加5ul  的缓冲液于50ul  的样品中，如果样品量不够或者不确定，缓冲液和样品的混合比例不要小于1:10即可混匀，静置1小时备用（如果标本是血清或者血浆，此步骤忽略）。

3. 洗液的配制：按1:100的比例配制洗液备用。

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人抗软骨抗体(anti-cartilage-Ab)ELISA试剂盒厂家】三. 仔细阅读说明书

1.检查试剂盒标签上的有效期。如果超过有效期，请勿使用。

2. 按说明书确定所有试剂齐全（数量、体积）

3. 标本制备要规范，每份标本体积要按2-3个复孔以上的量制备（贮存），尽量分装做备份。

4.短期内无法实验者，注意低温保存

5.准备好所有实验额外所需物品，（比如移液器，试管，清洗器，酶标仪）

6. 按说明书将所用试剂平衡至室温，根据检测标本数量确定所需试剂的量

7.检查试剂盒内每种试剂的贮存条件，保证所有试剂均按照试剂盒内说明书推荐的条件存储。 【人抗软骨抗体(anti-cartilage-Ab)ELISA试剂盒厂家】

8.检查不稳定或者变质的试剂溶液（比如沉淀或变色）。有些试剂，比如标准品稀释液或者检测稀释液，可能在设计时就含有沉淀物。所有试剂在滴入酶标板之前，必需充分混匀。而由于沉淀物的特性，在加入酶标板之前，必需不停搅拌。

9.保证充分的孵育时间和温度。

10.切勿使用不同生产批号的试剂取代现有试剂或混合现有试剂。

11.在混合或溶解蛋白溶液时，避免泡沫产生。

12.在实验开始之前，安排好实验流程。在实验开始之前，清洁工作台。

13.若有问题，应与我公司或代理商的技术支持联系

【人抗软骨抗体(anti-cartilage-Ab)ELISA试剂盒厂家】四、客户须知

齐一生物销售：021－6034 8496；181214 53965；173021 04490

1.在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。 

2.试剂盒代测标本的客户取材前须向我司销售人员索要说明书，具体操作注意事项请与我司技术人员沟通。

3.液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

4.购买前请提前告诉我们：您样本的种属、样本数量、待测指标及其它特殊要求。

【人抗软骨抗体(anti-cartilage-Ab)ELISA试剂盒厂家】五、代测数据详细内容

1、ELISA标准曲线；

2、实验具体详细步骤；

3、完整的实验报告（试剂耗材，实验方法，实验结果及结果说明）；

4、实验进行阶段，我公司客服人员会及时向您汇报实验进程。

QY-03220 AEBP1 (Adipocyte enhance binding protein 1)脂肪细胞增强结合蛋白1                0.5mg/6501mg/1100                                  

QY-03970 MMP-9(matrix metalloproteinase 9)基质金属蛋白酶-9(抗原)                0.5mg/6501mg/1100                                   【人抗软骨抗体(anti-cartilage-Ab)ELISA试剂盒厂家】

QY-01770 AGER（Advanced glycosylation end product-specific receptor）晚期糖基化终末产物特异性受体(抗原）               0.5mg/6501mg/1100                                  

QY-02370 14-3-3 protein14-3-3 蛋白（多肽抗原）                0.5mg/6501mg/1100                                  

QY-00370 AIF　（Apoptosis-Inducing Factor）调亡诱导因子(多肽片断抗原）                    0.5mg/6501mg/1100                                  

QY-02990 Adenylate Kinase 1 (AK-1)腺苷酸激酶-1                   0.5mg/6501mg/1100                                  

QY-00370 AIF　（Apoptosis-Inducing Factor）调亡诱导因子(多肽片断抗原）                    0.5mg/6501mg/1100                                  

QY-01150 Akt/PKB蛋白激酶B（抗原）                  0.5mg/7001mg/1200                                 

QY-08610 2,4-D/KLH（2,4-Dichlorophenoxyacetic acid）2，4-二氯苯氧乙酸偶联血蓝蛋白               5mg/1500                                   

QY-01150 Akt/PKB蛋白激酶B（抗原）                  0.5mg/7001mg/1200                                 

QY-00970 ALK （Anaplastic Lymphoma Kinase）（CD246Ag）间变型淋巴瘤激酶（抗原）                0.5mg/6501mg/1100                                  

QY-00070 AM Adrenomedullin 肾上腺髓质素（抗原）                 0.1mg/1800                               

QY-04160 TIMP-2(Tissue Inhibitor of Metalloproteinase-2)金属蛋白酶组织抑制因子-2(抗原)             0.5mg/6501mg/1100                                  

QY-04170 TIMP-3(Tissue Inhibitor of Metalloproteinase-3)金属蛋白酶组织抑制因子(抗原)                 0.5mg/6501mg/1100                                  

QY-00900 ADM R, Adrenomedullin receptor肾上腺髓质素受体（抗原）            0.5mg/6501mg/1100                                  

Yf-01580  Amylin1-37/FITC异硫氰酸荧光素(FITC)标记糖尿病相关肽1-37           1ml/2860                                     

QY-03060 VTG(vitellogenin)青鳉鱼卵黄蛋白                    0.1mg/6500.2mg/1100                              

QY-00580 Apaf-1 （Apoptosis protease activating factor-1）(CT)凋亡蛋白活性因子-1（抗原）                0.5mg/7001mg/1200                                  【人抗软骨抗体(anti-cartilage-Ab)ELISA试剂盒厂家】

QY-00590 Apaf-1 （Apoptosis protease activating factor-1）(NT)凋亡蛋白活性因子-1（抗原）               0.5mg/7001mg/1200                                 

QY-01180 AR （Androgen Receptor)雄激素受体（抗原）           0.5mg/6501mg/1100                                  

QY-00450 AT （Angiotenogen）血管紧张素原（抗原）              0.5mg/6501mg/1100                                  

QY-01670 Apo-E （Apolipoprotein E）载脂蛋白E（抗原）                   0.5mg/6501mg/1100                                  

QY-02450 IKI3 family protein             0.5mg/8001mg/1200                                  

QY-03170 ILK-1 (Integrin-linked protein kinase 1)整合素连接激酶-1               0.5mg/6501mg/1100                                  

QY-01140 Aromatase芳香化酶（抗原）                 0.5mg/6501mg/1100                                  

QY-00550 ART(Artemin)（抗原）             0.5mg/6501mg/1100                                  

QY-02610 AQP-2 （aquaporin Protein-2）水通道蛋白-2（抗原）                0.5mg/6501mg/1100                                  

QY-01640 BACE( ASP2 ） β分泌酶（抗原）                 0.5mg/6501mg/1100                                  

QY-02750 Bassoon(RCN)            0.5mg/6501mg/1200                                  

QY-01270 Bax （Bcl-2 Assaciated X protein） Bax（多肽抗原）                  0.5mg/6501mg/1100                                  

QY-00320 Bcl-2（抗原）Bcl-2（多肽抗原）                    0.5mg/6501mg/1100                                  

QY-02480 BDNF (Brain-derived Neurotrophin factor)脑源神经营养因子(脑衍化神经营养因子)                  0.5mg/6501mg/1100                                  

QY-02170 bFGF碱性成纤维细胞生长因子（多肽抗原）              0.5mg/6501mg/1100                                  

9018902  Loading Block 72 x 0.1 mL Multi-channel                  CNY                             

9018558  Door, w/o hood ａnd reflector, EZ1a                  CNY                             

9013112  Vacuum Manifold 2.03              CNY                             

670291     ipsogen BCR-ABL1 mbcr Standards          CNY                             

387016     Investigator Quantiplex Kit (200)              CNY                              【人抗软骨抗体(anti-cartilage-Ab)ELISA试剂盒厂家】

380327     Investigator Triplex DSF Kit (400)              CNY                             

9001492  EZ1 Advanced XL               CNY                             

9001230  LiquiChip Workstation IS 200             CNY                             

9000852  BioSprint 96               CNY                             

4880004  PG Influenza A RT-PCR Kit (48) LE Z                   CNY                             

130908     NeXtal DWBlock Cations Suite                   CNY                             

4559265  artus C.trachomatisPlus RG PCR Kit(96)CE                CNY                             

4801014  careHBV PCR ASSAY                   CNY                             

4803004  careHBV Mutation PCR ASSAY                   CNY                             

9240599  1Brake cylinder, Elevator, AP98                CNY                             

9240582  Filter, Regulator, AP96              CNY                             

9240559  Temp sensor, Cond., Eppendorf, AP196/98                 CNY                             

9240556  Shutter Door Assy, Eppendorf, AP196/98          CNY                             

9240538  Cable Assy, SiNug, Mtr-Control, AP96/196                 CNY                             

9240537  Controller N2, SiNug, AP96/196                 CNY                             

9240505  PC-104, AP196          CNY                              【人抗软骨抗体(anti-cartilage-Ab)ELISA试剂盒厂家】

9240244  Cap, brushes, TR                CNY                             

9240103  Re-Installation, BR MDx DSP   The Re-Installation, BR MDx DSP facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9240046  Thermostate unit, 220V, 1.04          CNY                             

9239556  Assy,Kit,Retrofit,LaserDriverBoardUnlim                  CNY                             

9020027  Sensor, solenoid, 170mm, EZ1a                 CNY                             

9019500  UV light system, CAS                  CNY                             

9019499  ReagBlock, 8x1.5ml/2ml/1.5ml Ta/Flat/Ta                CNY                             

9241225  QIAcube, Inspection Service    "The Annual Inspection Service, QIAcube is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist. During the half day-long procedure, all application-critical modules of the QIAcube are inspected ａnd tested. Performed tests ａnd test results are documented in a Visit Report."  CNY                             

9241218  HC2, Full Agreement, no PM   "Service response time (on-site repair):not applicable

Periodic inspection/maintenance: no, not included

Cost coverage depot repair (parts, labor, packaging, shipping): yes, full coverage

For defect DML, after customer calling QIAGEN technical service hotline before 12 am:

- A special package containing a replacement unit shall be sent to the customer within 2 working days.

- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.  

For DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer ａnd Votexer:

- A new unit shall be sent to customer within  7-10 working days at no extra cost

- Customer shall send the defect unit to QIAGEN for disposal

- On-site installation of PC ａnd application software is not covered by the agreement"         CNY                             

9241182  Belt, Y, QX                  CNY