价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供优质产品。

【兔子肠脂肪酸结合蛋白(iFABP)ELISA试剂盒现货】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【兔子肠脂肪酸结合蛋白(iFABP)ELISA试剂盒现货】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【兔子肠脂肪酸结合蛋白(iFABP)ELISA试剂盒现货】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【兔子肠脂肪酸结合蛋白(iFABP)ELISA试剂盒现货】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9019402  Transformer, heater, RG3                  CNY                              【兔子肠脂肪酸结合蛋白(iFABP)ELISA试剂盒现货】

1027321  Mm/Hs_MAPK1 control siRNA (20 nmol)                  CNY                             

9019329  Serial connector USB, internal cable, RG                  CNY                             

9019238  Heating block, PyroQ24            CNY                             

9020478  Heating plate, GSC           CNY                             

1026965  PCR Buffer,10 x (1,2/2) without MgCl2              CNY                             

9233466  Coupling, CPC, inline, male, 6mm             CNY                             

9233452  Coupling, X motor, BR8000               CNY                             

9019217  Lead screw - Y robot, PyroQ96MD           CNY                             

1017635  Protease Solvent 6x (10,2/15), Azid,KG             CNY                             

1066846  AIV-H5 RT-PCR REAGENT LE             CNY                             

1054250  Effectene Transfection Reag. Kit (0.3ml)                   CNY                             

1061051  HBV PCR REAGENT II                CNY                             

9019890  Foam insert, rotor securing, QIAC            CNY                             

9019861  Rotor Locking Nut Set               CNY                             

9019821  IQ/OQ document, QS                CNY                             

9019768  Sensor, interlock2, with cable, QX            CNY                              【兔子肠脂肪酸结合蛋白(iFABP)ELISA试剂盒现货】

9019765  Belt, Y, V2, QX           CNY                             

9019727  Board, transport conversion, V2, QX                 CNY                             

9013523  Underlay, vaccum manifold, v2.01           CNY                             

9013447  Control unit Y/Z, 4 channel                CNY                             

9013431  Maintenance kit, semi annual, BR9600 (E)               CNY                             

9238807  Round carrier for 12 Tubes 15ml               CNY                             

9019715  PyroMark Q24 User Manual             CNY                             

9019648  Mixer module w. top plate, PyroQ96ID            CNY                             

9019643  Sensor, solenoid, EZ1xl              CNY                             

9019589  Cable harness, x axis, C34                  CNY                             

9019587  PCB, mainboard, QGY               CNY                             

9019582  End cap, Y-arm, Cxx          CNY                             

9019551  Fuse, 2A            CNY                             

9019513  Adapter, reagent tub plate, CX1               CNY                             

9019502  TANK UPPER BUFFER ASSY GS          CNY                             

QKG-254  50×TAE  规格：500ml                                        【兔子肠脂肪酸结合蛋白(iFABP)ELISA试剂盒现货】

QKG-255  5×TBE    规格：500ml                                       

QKG-256  Lysozyme 规格：100mg                                     

QKG-257  DNase I (RNase Free)       规格：1000U                                      

QKG-258  NGS Fast DNA Library Prep Set for Illumina     规格：24次                                                   

QKG-259            规格：96次                                        

QKG-260  二代测序接头引物试剂盒I  规格：24次                                                   

QKG-261            规格：96次                                        

QKG-262                                                         

QKG-263  二代测序接头引物试剂盒II (Illumina) 规格：24次                                                   

QKG-264            规格：96次                                        

QKG-265  细菌蛋白抽提试剂盒 Bacterial Protein Extraction Kit         规格：25次                                        

QKG-266            规格：100次                                              

QKG-267  哺乳动物蛋白抽提试剂盒 Mammalian Protein Extraction Kit    规格：25次                                        

QKG-268            规格：100次                                              

QKG-269  酵母蛋白抽提试剂盒 Yeast Protein Extraction Kit       规格：25次                                        

QKG-270            规格：100次                                               【兔子肠脂肪酸结合蛋白(iFABP)ELISA试剂盒现货】

QKG-271  组织蛋白抽提试剂盒  Tissue Protein Extraction Kit                                                 

QKG-272  组织蛋白抽提试剂盒 Tissue Protein Extraction Kit     规格：25次                                        

QKG-273            规格：100次                                              

QKG-274  Nc-细胞核/浆蛋白抽提试剂盒 Nuclear ａnd Cytoplasmic Extraction Kit      规格：50次                                            

QKG-275  植物蛋白提取试剂盒 Plant Protein Extraction Kit       规格：25次                                        

QKG-276  RIPA Lysis Buffer (Strong)          规格：100ml                                             

QKG-277  RIPA Lysis Buffer (Medium)      规格：100ml                                             

QKG-278  RIPA Lysis Buffer (Mild)    规格：100ml                                             

QKG-279  SDS Lysis Buffer         规格：100ml                                             

QKG-280  包涵体蛋白溶解液 Inclusion Body Denature Reagent          规格：100ml                                             

QKG-281  蛋白酶抑制剂混合物 Protease Inhibitor Cocktail (100×)   规格：1ml                                             

QKG-282  蛋白磷酸酶抑制剂混合物 Phosphatase Inhibitor Cocktail (100×)     规格：1ml                                             

QKG-283  Ni-Agarose His标签蛋白纯化试剂盒(可溶性蛋白)      规格：5ml