产品介绍
小鼠脂多糖/内毒素(LPS)ELISA试剂盒价格
价 格:¥电议
型 号:
产品完善度:
生产地:其他访问量:28次
发布日期:2016/8/4 17:41:07
更新日期:2016/8/4 17:41:07
详细内容
1. 酶标仪(450nm 波长滤光片)
2. 高精度移液器,EP 管及一次性吸头
3. 37℃恒温箱,双蒸水或去离子水
4. 吸水纸
【友情提示】:本产品仅供科研研究使用,不得用于人体临床直接检测。避免给您带来不必要的损失,请仔细阅读购买说明!【小鼠脂多糖/内毒素(LPS)ELISA试剂盒价格】
ELISA应用:
ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎(HEV)病毒IgM抗体ELISA检测
【小鼠脂多糖/内毒素(LPS)ELISA试剂盒价格】齐一生物销售:021-6034 8496;181214 53965;173021 04490 web:www.qiyibio.com
试剂盒安全性提示:
1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体,请尽快用水冲洗。
2.实验中不要吃喝、抽烟或使用化妆品。
3.不要用嘴吸取试剂盒里的任何成份。
样本保存条件:
1.2-8℃冰箱多放2周
2.-20到-50℃的冰箱可以放3-6个月
3.-50℃以下的冰箱,可以放一年
【小鼠脂多糖/内毒素(LPS)ELISA试剂盒价格】ELISA实验原理
试剂盒是固相夹心法酶联免疫吸附实验(ELISA).已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.
免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.
HBsAg试剂特点(检测模式:临床抗体夹心法):包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性(99.98%)提高检测的灵敏度,应用ParlEhrich学说(PEI)HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml
ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP(辣根过氧化物酶)酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性.
以上资料仅供参考了解,不供于论文写作
【小鼠脂多糖/内毒素(LPS)ELISA试剂盒价格】齐一生物专业为您提供高品质,高灵敏度.高质量的酶联免疫试剂盒,齐一生物长期经营ELISA试剂盒(免费代测)及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售:021-6034 8496;181214 53965;173021 04490欢迎选购!
9241768 QIAxtractor, Basic Agreement The Basic Cover Agreement, QIAxtractor provides full service coverage for the QIAxtractor and the CAS 1280. Costs for service visits are fully covered including travel, labor, and parts. One on-site pipetting head service and inspection is included. With a Basic Cover Agreement, there is a response time of 5 working days. CNY
9241656 Transfer Rack Plate, AP CNY
9241630 Shaker Adjustment Kit, AP CNY
9241367 Damper, door, EZ1a CNY
9240759 Update, Sliding Door Latch, AP98 CNY
9243548 Pyro Q96 ID, PM Service "Service response time (repair): not applicable
Periodic inspection/maintenance: yes
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts : not included
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage" CNY
9242766 Cogwheel, x-drive, portal, Qsym CNY 【小鼠脂多糖/内毒素(LPS)ELISA试剂盒价格】
9242710 Validation Support, 2d, QIAgility The QIAGEN QIAgility Guided Validation Support service includes all travel and labor charges for a site visit up to 2 days by a QIAGEN Specialist. A QIAGEN specialist will provide an introduction to the QIAgility system and software, review the User Manual, and practical hands-on operation. The Guided Validation service includes experimental design consultation, internal validation documentation support, and QIAGEN sponsored data templates. Enough consumable chemistry and accessories (QIAGEN or other vendors) should be purchased to cover the validation experimental design and are not included in the cost of validation support. Please indicate which processes (quant setup, normalization, PCR setup, CE setup) and kit manufacturer (Applied Biosystems, Promega, QIAGEN) need to be included in the validation. CNY
9242253 Cable, x, portal AS, Qsym CNY
9242234 RGQ, IQ/OQ Service "Instrument qualification support service for the Rotorgene Q and Rotorgene 6000. The product contains the IQ/OQ documentation and the performance of the qualification protocols (travel and labor costs included).
During the service, the compliance with the manufacturer's installation requirements is testified and the performance verification & dynamic calibration of the Rotorgene Q is performed." CNY
9242172 Repair fee, Depot Repair, TissueLyser II CNY
5000-1403 Bumpers Side, VTX2 CNY
5000-1401 Guide Rack Back Asmbly, VTX2 CNY
5000-1311 Capacitor 8UF, SKR1 CNY
5000-1273 Door Damper Assembly, DML2 CNY
5000-1258 Y Optical Sensor CMPLT, DML2 CNY
5050-1074 V.1 CD Rom Manual - LumiCheck Plate CNY
5050-1069 V.1 CD Rom Manual - Rotary Shaker I, 240V CNY
6000-5001 Costar Plate Lids CNY
6000-3120 Plate Washer I - WasteReserv.CapAssembly CNY
5000-1420 Driveplate Asmly 099A, VTX2 CNY
5127-1220 hc2 Sample Conversion Kit CNY
9243701 careHPV, Training CNY 【小鼠脂多糖/内毒素(LPS)ELISA试剂盒价格】
EA11423 EASYartus BKV RG_I CNY
800190 Sequencing in a Box Service CNY
800110 Single Read, w/ prep "Bacterial clone cultivation and template purification or PCR product purification, including DNA quality control
Repetition of sequencing, if necessary
Data collected to maximal read length" CNY
132538 NeXtal Stock Dioxane (50) 100% v/v Dioxane - 50mL (5 x 10 ml tube) CNY
338162 RT2 PCR Array Loading Reservoir CNY
338114 Mouse XpressRef Universal Total RNA CNY
203743 QIAGEN Fast Cycling PCR Kit (200) For 200 x 20 µl reactions: 2 x 1 ml Fast Cycling Master Mix, 2x (containing dNTPs, patent pending buffer composition), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution CNY
QY-x1669 小鼠白血病细胞C3
QY-x1670 小鼠β胰岛素瘤细胞RIN-m5F
QY-x1671 小鼠自发高乳腺癌细胞TA2
QY-x1672 小鼠淋巴瘤细胞Nb2-11
QY-x1673 小鼠骨髓瘤细胞NS-1
QY-x1674 小鼠肾癌细胞Kert-3
QY-x1675 HPVl6 E6、E7和ras基因共转化的C57BL/C(H-2b)小鼠肺上皮细胞TC-1
QY-x1676 长臂猿淋巴瘤MLA-144
QY-x1677 细胞名称 细胞代号 ATCC号码 组织来源 培养基 ATCC描述/中科院细胞库描述
QY-x1678 人胚肾细胞 293T CRL-11268? 肾 DMEM+10%FBS
QY-x1679 人肾上皮细胞系 293 肾 DMEM+10%FBS
QY-x1680 人肾上皮细胞系 293H 肾 DMEM+10%FBS
QY-x1681 人脐静脉内皮细胞 HUVEC 脐带静脉 ECM或
QY-x1682 EGM-2 【小鼠脂多糖/内毒素(LPS)ELISA试剂盒价格】
QY-x1683 人胚肺成纤维细胞 HLF 胚胎;肺 MEM α(+)+10%FBS
QY-x1684 人胚肺二倍体细胞 CCC-HPF-1 胚胎;肺 DMEM+ 10% FBS
QY-x1685 人微血管内皮细胞 MVEC 微血管 DMEM+ 10%NBCS
QY-x1686 人支气管平滑肌细胞 HBSMC RPMI 1640 + 10% NBCS
QY-x1687 人肝癌细胞 Hep G2 HB-8065? 肝细胞癌 DMEM+10%NBCS
QY-x1688 人肝癌细胞 BEL-7402 肝癌 RPMI 1640 + 10% NBCS
QY-x1689 鼠肝癌细胞 H22 (悬浮) 肝癌 RPMI 1640 + 10% NBCS
QY-x1690 人肺腺癌
QY-x1691 人肺癌细胞 SPC-A-1 肺癌 RPMI 1640 + 10% FBS
QY-x1692 人肺癌细
QY-x1693 人非小细胞肺癌细胞 A549 CCL-185? 肺癌 RPMI 1640 + 10% NBCS
QY-x1694 人肺癌细胞 GLC82 肺癌
QY-x1695 人小细胞肺癌细胞