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1。我必须运行所有的标准和样品的重复？

是的，重复的运行，以监测测定的精度和提高检测结果的信心。

2。我必须在一次运行所有的样品吗？

不，每个试剂盒采用stripwell微孔板。这允许用户在不同的时间来分析不同数量的样品。

3。什么类型的可重复性的结果，得到与分析？

每一个工具都有一个包含一个典型数据的图表的手册。在运营商的任何变化，移液和洗涤技术，培养时间、温度、试剂盒的年龄可以引起变化的结果。每个用户都应该得到自己的标准曲线。

4。是否有可能储存的试剂比表示？

在不推荐使用条件下的部件组件的存储，以保证测试的正确性能。

5。我应该如何储存我的样品？

样品应保存在-20°C或更低的温度。长期储存，建议冻结他们在- 70余。

6。我可以修改协议吗？

BG的ELISA试剂盒进行了优化，提供佳的结果。修改的格式或协议可能会给出错误的结果。

7。我可以使用一个不建议在试剂盒插入的样本类型吗？

该试剂盒已被验证在试剂盒插入的样品类型。未经检验的样品类型。为进一步信息的联系技术服务。

8。我的样本产生的值，在动态范围内的测定。我可以使用这些值吗？

建议只有在标准曲线的范围内下降的样本值。标准曲线的范围内的值一般是非线性的，这可能导致不正确的外推值。产生值高于标准的样品应为（进一步）稀释和重复。如果样品低于该测定的范围内，该样品被认为是不可检测的。

9。我每次都要运行一个空白或零标准吗？

是的，这些都是必需的计算，并反映任何微妙但显着的性能变化，从一天到一天，检测到检测。当排除特定的检测问题的来源时，它们也非常有帮助。

10。我能改变我在检测中使用的样本量吗？

不建议你改变卷由于BG工具被设计为佳的性能在给定的体积

11。可以使用不同的组件组件吗？

每一个组件包含有特定批号的组件，以确保所有的组件都进行优化，以及与组件中的所有其他组件。对这些特定的地段进行质量检验。这是从来没有建议使用你自己的组件或组件从其他包或供应商。

12。我的标准曲线看起来很好，但我没有得到一个信号，我的样本，当我预期，为什么？

样品不包含被分析物。一个矩阵的效果可能是掩盖检测。确保推荐的稀释液在试剂盒插入后按规定进行。如果建议稀释，请检查是否正确进行稀释。过稀释会导致样品在标准曲线的范围内下降。

13。你怎么推荐我洗盘子？

如果您使用的是一个自动平板洗衣机，我们建议定期检查校准，并将该系统在洗涤前冲洗缓冲液冲洗。手动洗衣机也是一样的。一个中继器或洗瓶也可以使用。用户应小心，以确保所有的内容都是送气和板拍干的无尘纸。【大鼠胆固醇7α-羟化酶（CYP7a1）ELISA试剂盒现货】

14。我需要用一个盘子吗？

可靠的结果可以得到无板振动，但外径的一般会低于那些使用平板振动器。

15。为什么我要用波长校正450-570nm之间？

对于酶联免疫吸附试验，在双波长读的是做正确的光密度贡献的塑料以及，灯和光学波动。

16。如果我提我的样本，我还需要遵循推荐的稀释液试剂盒中插入了吗？

提取过程中所需的样本稀释量将受到影响的纯化和浓度的影响的协议使用。稀释或浓缩的金额将由终用户决定。

17。什么是预期的分析物浓度，我应该找到？

一个给定的分析物的量可能会有所不同，不仅从物种的物种，但也组织和细胞来源。这个信息的佳来源是目前的文献，很容易通过互联网在多个科学数据库访问。

18。我的光密度有点高（或较低），比那些在手册中，与我的试剂盒来了。为什么？

光学密度受影响

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【大鼠胆固醇7α-羟化酶（CYP7a1）ELISA试剂盒现货】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【大鼠胆固醇7α-羟化酶（CYP7a1）ELISA试剂盒现货】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【大鼠胆固醇7α-羟化酶（CYP7a1）ELISA试剂盒现货】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

800160     Single Read, w/o prep      "Sequencing of plasmid DNA ａnd PCR products, including DNA quality control

Repetition of sequencing, if necessary

Data collected to maximal read length" CNY                             

800101     Basic Read Tubes               CNY                             

765512     PAXgene Tissue STABILIZER Conc. (150 ml)              CNY                             

132007     EasyXtal 15-well Tool (100)                CNY                             

201207     Taq DNA Polymerase (5000)    "20 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

9238319  V-drive unit, BR8000, V2          CNY                             

9238178  QIAplate Holder, SBS, black              CNY                             

9238134  Multiwell-Plate Holder, SBS              CNY                             

9240675  Tee, Barbed, Plastic, AP96                CNY                              【大鼠胆固醇7α-羟化酶（CYP7a1）ELISA试剂盒现货】

9240653  Pressure Pump, Gast, 120V, AP96            CNY                             

9240624  Solid State Relay, AP196/98               CNY                             

9240576  Cable Assy, Capper, X51,CCW, AP96/196           CNY                             

9240546  Motor, Shutter Door, Eppendorf, AP196/98               CNY                             

9240544  Centrifuge, 5810RA, Eppendorf, AP196/98                CNY                             

9240513  Mouse, Standard, AP                 CNY                             

9240508  Software & Std. Protocols, AP98               CNY                             

9240499  Reagent Pump, AP            CNY                             

9240378  Autopure, Installation      The Installation, AUTOPURE facilitates the quick implementation of the AUTOPURE into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the AUTPOURE instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9240242  Connector, disp. tip adapter drive, TR              CNY                             

9239959  EZ1, Premium Agreement       The Premium Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).         CNY                             

9020028  ZB sensor, 320mm, EZ1xl                   CNY                             

9020018  Upgrade kit, vacuum chamber, CAS-1820                 CNY                             

9019987  PCB, small breakout, 8 core, Cxx               CNY                             

9019927  Cover, tip ejector (UV), Cx8               CNY                             

9019926  PCB, Microboard, Cxx                CNY                              【大鼠胆固醇7α-羟化酶（CYP7a1）ELISA试剂盒现货】

9019488  Filter, polycap incl. tube/coupling, CX1             CNY                             

9238856  #Basic Cover Agreement MDx, 2PM, 1yr         The Basic Cover Agreement MDx, 2PM provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes two annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9238839  #Twister II, Basic Agreement, no PM      The Basic Cover Agreement BR Twister II provides full service coverage for the BioRobot Twister II. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Cover Agreement, the response time is 5 working days.     CNY                             

9241178  Fuse, 4A, 250VAC, QX                CNY                             

9238830  BR 3000, Basic Agreement      The Basic Cover Agreement 3000 provides full service coverage for the BioRobot 3000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238813  Rectangular carrier for 20 RIA-tubes                CNY                             

QYS-03083        总巯基测试盒         100管/48样    酶标法                                

QYS-03084        超氧化物歧化酶SOD测试盒         50管/48样      可见分光光度法                                

QYS-03085        超氧化物歧化酶SOD测试盒         100管/96样    酶标法                                

QYS-03086        过氧化氢酶CAT测试盒          50管/48样      紫外分光光度法                                

QYS-03087        过氧化物酶POD测试盒        50管/48样      可见分光光度法                                

QYS-03088        多酚氧化酶PPO测试盒         50管/24样      可见分光光度法                                

QYS-03089        多酚氧化酶PPO测试盒         100管/48样    酶标法                                

QYS-03090        苯丙氨酸解氨酶PAL测试盒 50管/48样      紫外分光光度法                                

QYS-03091        苯丙氨酸解氨酶PAL测试盒 100管/96样    酶标法                                

QYS-03092        脯氨酸PRO含量测试盒         50管/48样      可见分光光度法                                

QYS-03093        ATP含量测试盒      50管/48样      高效液相色谱法                                

QYS-03094        ADP含量测试盒     50管/48样      高效液相色谱法                                

QYS-03095        AMP含量测试盒    50管/48样      高效液相色谱法                                

QYS-03096        Na+k+——ATP酶测试盒        50管/24样      可见分光光度法                                

QYS-03097        Na+k+——ATP酶测试盒        100管/48样    酶标法                                 【大鼠胆固醇7α-羟化酶（CYP7a1）ELISA试剂盒现货】

QYS-03098        Ca++Mg++——ATP酶测试盒         50管/24样      可见分光光度法                                

QYS-03099        Ca++Mg++——ATP酶测试盒         100管/48样    酶标法                                

QYS-03100        硝酸还原酶测试盒         50管/24样      可见分光光度法                                

QYS-03101        硝酸还原酶测试盒         100管/48样    酶标法                                

QYS-03102        谷氨酸合成酶GOGAT测试盒        50管/48样      紫外分光光度法                                

QYS-03103        谷氨酰胺酶GLS测试盒          50管/48样      可见分光光度法                                

QYS-03104        谷氨酸脱氢酶GDH测试盒   50管/48样      紫外分光光度法                                

QYS-03105        NO含量测试盒       50管/24样      可见分光光度法                                

QYS-03106        NO含量测试盒       100管/48样    酶标法                                

QYS-03107        水土中亚硝酸盐含量测试盒         50管/48样      可见分光光度法                                

QYS-03108        水土中亚硝酸盐含量测试盒         100管/96样    酶标法                                

QYS-03109        食品中亚硝酸盐含量测试盒         50管/48样      可见分光光度法                                

QYS-03110        食品中亚硝酸盐含量测试盒         100管/96样    酶标法                                

QYS-03111        谷氨酰胺合成酶GS测试盒  50管/24样      可见分光光度法                                

QYS-03112        谷丙转氨酶GPT测试盒         50管/24样      可见分光光度法                                

QYS-03113        谷丙转氨酶GPT测试盒         100管/48样    酶标法                                 【大鼠胆固醇7α-羟化酶（CYP7a1）ELISA试剂盒现货】

QYS-03114        谷草转氨酶GOT测试盒        50管/24样      可见分光光度法                                

QYS-03115        谷草转氨酶GOT测试盒        100管/48样    酶标法