价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供优质产品。

【大鼠Na-K-ATP酶ELISA试剂盒厂家】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【大鼠Na-K-ATP酶ELISA试剂盒厂家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【大鼠Na-K-ATP酶ELISA试剂盒厂家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【大鼠Na-K-ATP酶ELISA试剂盒厂家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9011481  BGR Greiferkopf Plato 1xxx               CNY                             

6000-1240U     Microplate Heater I, 240V                CNY                             

9011334  Arm Mechanism, RCS1              CNY                             

330514     RT² SYBR Green Fluor qPCR Mastermix (6)               CNY                             

9011092  Mounting plate, power supplies, BR8000                 CNY                             

150023     REPLI-g Mini Kit (25)        DNA Polymerase, Buffers, ａnd Reagents for 25 x 50 µl whole genome amplification reactions (typical yield 10 µg per reaction)     CNY                             

9241806  Pyro Q24, Installation      Installation ａnd Introductory Training PyroMark is an on-site service, conducted by a QIAGEN Service Specialist. It covers the installation ａnd commissioning of the instrument ａnd the handling of the PyroMark systems hardware. It trains the user on the operation of the PyroMark software ａnd the setup ａnd execution of a pyrosequencing run. After the training the user will be able to prepare samples, set-up ａnd run the instrument, analyse the results ａnd design new assays as well as troubleshoot new assays ａnd perform basic maintenance on the sample preparation tool ａnd instrument. All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements         CNY                             

9241177  Power switch, 6A, 250VAC, QX                  CNY                             

9241147  Transport  lock, X axis, M48            CNY                             

9241032  Tube insert, 2.0 ml,samp.carr., Qsym               CNY                             

9240986  PCB, main, cooling device, Qsym              CNY                             

9240981  Slide block, sample tracking, Qsym           CNY                             

9240974  Carrier locking, sample tracking, Qsym            CNY                             

9240967  Sample tracking, Qsym             CNY                             

9240963  Drop catcher, pipetting system, Qsym              CNY                             

9240944  Motor, xy, portal, Qsym            CNY                              【大鼠Na-K-ATP酶ELISA试剂盒厂家】

9234853  Modem, analoge ELSA              CNY                             

9240763  Analog Module, F2-04AD-1, AP96            CNY                             

9241376  Upgrade, Autopure LS Software v3.x       The “Upgrade, Autopure LS Software v3.x” is an on-site service for the software upgrade to the latest Autopure LS software version (currently version 3.1), the verification of the instrument performance (including the setting of database parameter), ａnd a user training. The product is available for Autopure LS instruments (Version AP-98) with a current software version lower or equal to V2.4 ａnd is performed by a QIAGEN service specialist.       CNY                             

9241764  QIAgility, Premium Agreement        "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) or PC repair provision: not included

Transportation cost coverage: not applicable"        CNY                             

9241757  Certification, QIAsymphony              CNY                             

9241464  USB Memory Stick 2GB, Qsym                   CNY                             

9241370  Power supply, EZ1a           CNY                             

9241363  Ribbon cable, CPU board, EZ1a                 CNY                             

9240756  Downtube, 500 ml Bottle, AP98               CNY                             

9240748  Belt, Dispense Head, AP           CNY                             

9243543  BR Universal, PM Service         "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9243308  HC2 Systems Training – In House     HC2 In house, 3 day course trains users on the HC2 system (manual or automated) ａnd includes participate travel, lodging, meals, materials ａnd PACE continuing education credits.      CNY                             

9242861  QIArent&go – Rent out fee QIAxcel Adv.                  CNY                             

9242544  Power Input Module, Eppendorf, AP196/98              CNY                             

9242507  QIAsymphony SPAS, Full Agreement                  CNY                             

QYS-03314        成纤维细胞转染试剂     FibroblastTransfectionKit         200Transfections                                 

QYS-03315        上皮细胞转染试剂盒     EpithelialCellTransfectionKit    100Transfections                                 

QYS-03316        间充质干细胞转染试剂盒     MesenchymalStemCellTransfectionKit    250Transfections                                 

QYS-03317        星形胶质细胞转染试剂盒     AstrocyteTransfectionKit          250Transfections                                 

QYS-03318        平滑肌细胞转染试剂盒         SmoothMuscleCellTransfectionKit   250Transfections                                 

QYS-03319        角质细胞转染试剂盒     KeratinocyteTransfectionKit    200Transfections                                 

QYS-03320        上皮细胞转染试剂盒     EpithelialCellTransfectionKit    250Transfections                                 

QYS-03321        体外管腔形成分析试剂盒     InVitroTubeFomationAssayAngiogenesisAssay        50tests/96wellplate                            【大鼠Na-K-ATP酶ELISA试剂盒厂家】

QYS-03322        I型胶原细胞粘附检测试剂盒       CollagenICellAdhesionAssay     48tests/48wellplate                            

QYS-03323        纤维粘连细胞粘附检测试剂盒     FibronectinCellAdhesionAssay 48tests/48wellplate                            

QYS-03324        胶原I-3D凝胶试剂盒    CollagenI-3DGellingKit    100mg                                 

QYS-03325        胶原I-细胞培养表面包被试剂盒 CollageI-CellCultureSurfaceCoatingKit    10mg                                   

QYS-03326        MTT细胞活力和增殖检测试剂盒         MTTCellViabilityandProliferationAssay   1000tests/96wellplate                                

QYS-03327        WST-1细胞活力和增殖检测试剂盒      WST-1CellViabilityandProliferationAssay          1000tests/96wellplate                                 

QYS-03328        细胞衰老检测试剂盒     CellSenescenceAssay        50tests/53mmplate                            

QYS-03329        LDH细胞毒性检测试剂盒     LDHCytotoxicityAssay      500tests/96wellplate                                   

QYS-03330        TUNEL色标法细胞凋亡检测试剂盒     ColorimetricTUNELApoptosisAssay 50tests/96wellplate                            

QYS-03331        活/死细胞检测试剂盒   Live/DeadCellStainingKit 1000tests/96wellplate                                

QYS-03332        细胞凋亡蛋白酶-3分析试剂盒     Caspase-3Assay        100tests                              

QYS-03333        支原体PCR检测试剂盒         MycoplasmaPCRDetectionKit 100tests                              

QYS-03334        β-半乳糖苷酶比色检测试剂盒   Beta-GalactosidaseColorimetricAssay     50tests/53mmplate                            

QYS-03335        LDH细胞毒性检测试剂盒     LDHCytotoxicityAssay      500tests/96wellplate                                   

QYS-03336        硝基苯酸钠pNPP磷酸酶检测试剂盒  pNPPPhosphataseAssay   500tests/96wellplate                                   

QYS-03337        比色法GAPDH分析试剂盒   ColorimetricGAPDHAssay         100tests/96wellplate                                    【大鼠Na-K-ATP酶ELISA试剂盒厂家】

QYS-03338        超氧化物歧化酶分析试剂盒         SuperoxideDisutaseAssay         100tests                              

QYS-03339        过氧化氢酶活性分析试剂盒         CatalaseActivityAssay      100tests                              

QYS-03340        谷胱甘肽过氧化物酶分析试剂盒         GlutathionePeroxidaseAssay   100tests                              

QYS-03341        细胞色素C氧化酶含量试剂盒     CytochromeCOxidaseAssay      100Testsincuvette                              

QYS-03342        柠檬酸合成酶的检测试剂盒         CitrateSynthaseAssay       100Testsincuvette                              

QYS-03343        谷氨酸脱氢酶检测试剂盒     GlutamateDehydrogenaseAssay     100Testsin96-wellplat                                 

QYS-03344        己糖激酶检测试剂盒     HexokinaseAssay      100Testsin96-wellplat                                 

QYS-03345        萄糖-6-磷酸磷酸脱氢酶检测试剂盒    Glucose-6-phosphateDehydrogenaseAssay     100Testsin96-wellplat                                 

QYS-03346        黄嘌呤氧化酶分析试剂盒     XanthineOxidaseAssay     100Testsin96-wellplat