价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供优质产品。

【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9237124       Upgrade App., MinElute 96       Take advantage of our instrumentation ａnd molecular biology expertise to install MinElute 96  on your BioRobot System.  The onsite protocol development visit includes all travel ａnd labor for the visit to install the standard QIAGEN MinElute 96 protocol, plus a MinElute 96 BioRobot Kit (4-plate) Cat # 28051 CNY                           

9237099       Upgrade BR configuration, on site            CNY                          

9236424       Motor, rotary, TWI             CNY                          

9232970       YZ 96 Connector Board 2 (Arm), E10103            CNY                          

9236409       Plate Stacker TW I, microplate         CNY                          

9236357       Sample Holder Large, 1.5ml             CNY                           【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

9232911       Board, YZ driver         CNY                          

9236336       Analog Board              CNY                          

9236158       Pot. , syr. or vert. , kit, RapidPlate           CNY                          

9236149       Locator Plate, 96 well,pos 4-5,BR RP            CNY                          

9236114       O-Ring, Coupling, RoboVac, BR8 RD v4.2           CNY                          

9236107       Flange Tool          CNY                          

9230680       Probe, 0.4 mm, BR8000 v1.01         CNY                          

9230478       Rack f. Diamend Gel Cards (holds 6cards)            CNY                          

9230473       Set, Cables, PCB (X-main) to shaker             CNY                          

9235646       Connector, Board, HS shaker, BR8000 v1.0         CNY                          

9235255       T-piece, HSPS tubing, connector             CNY                          

9235190       Motor, clamp, shaker plate,  BR8000           CNY                          

9230060       Rack for reagents (12 x diameter 21mm)         CNY                          

9238673       Hinge, imbalance system            CNY                          

9238653       Power Board, temp. switch - heat sink            CNY                          

9238635       Hook identification compl.         CNY                           【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

9242028       RGQ, Repair Fee "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"    CNY                          

9241821       Pyro Q96 MDa, Basic Agreement    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"  CNY                          

9241786       QIAgility Demopool Box Light Version           CNY                          

9240976       PCB, display, sample tracking, Qsym              CNY                          

9240968       Barcode camera,  sample tracking, Qsym             CNY                          

9240816       #Full Agreement, EZ1 DSP, 1YR             CNY                          

9240680       Spill Tray, Shaker,Modified, AP        CNY                          

9240790       Update,Waste Bottle Sensor, AP196/98         CNY                          

6000-5014    Lumicheck Plate Service Package, DML2             CNY                          

TGS       滋养层细胞生长因子     Trophoblast Growth Supplement       5 ml                     

PAdDS  前脂肪细胞分化生长因子     Preadipocyte Differentiation Supplement                             

PAGS    前脂肪细胞生长因子     Preadipocyte Growth Supplement     5 ml                      【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

AdGS    脂肪细胞生长因子   Adipocyte Growth Supplement  5 ml                     

OEpiCGS     卵巢上皮细胞生长因子  Ovarian Epithelial Cell Growth Supplement     5 ml                     

MODS   间充质干细胞成骨分化生长因子       Mesenchymal Stem Cell Osteogenic Differentiation supplement    5 ml                     

MADS   间充质干细胞脂肪分化生长因子       Mesenchymal Stem Cell Adipogenic Differentiation supplement    5 ml                     

MSCGS 间充质干细胞生长因子  Mesenchymal Stem Cell Growth Supplement  5 ml                     

MSCGS-sf    间充质干细胞生长因子  Mesenchymal Stem Cell Growth Supplement-serum free      5 ml                     

MSCGS-acf  间充质干细胞生长因子-无动物组分  Mesenchymal Stem Cell Growth Supplement-animal component free 5 ml                     

MCDS   间充质干细胞软骨分化生长因子       Mesenchymal Stem Cell Chondrogenic Differentiation Supplement      5 ml                        

MEpiCGS     乳腺上皮细胞生长因子       Mammary Epithelial Cell Growth Supplement  5 ml                     

FBS       胎牛血清     Fetal Bovine Serum, Human Cell Tested   10 ml                          

FBS       胎牛血清     Fetal Bovine Serum, Human Cell Tested   25 ml                          

T/E  胰酶/EDTA消化液   Trypsin/EDTA solution 100 ml                        

TNS       胰酶中和液 Trypsin Neutralization Solution   100 ml                        

TNS-sf 胰酶中和液-无血清  TNS-sf (Trypsin Neutralization Solution-serum free)      100 ml                        

ECDS    非酶细胞裂解液       Enzyme-Free Cell Dissociation Solution   100 ml                        

CFM      细胞冻存液 Cell Freezing Medium  50 ml                           【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

SF-CFM       无血清细胞冻存液        Serum-Free Cell Freezing Medium    50 ml                          

StemCryo      人胚胎干细胞冻存液      Human Pluripotent Stem Cell Cryopreservation Medium       50ml                           

StemCryo      人胚胎干细胞冻存液      Human Pluripotent Stem Cell Cryopreservation Medium,      5 x 10mL                          

TB  台盼蓝  Trypan Blue, 0.4%       50 ml                          

DPBS    磷酸盐缓冲液   Dulbecco’s Phosphate-Buffered Saline  500 ml                        

HBSS    平衡盐溶液       Hank＇s Balanced Salt Solution    500 ml                        

PLL       多聚赖氨酸       Poly-L-Lysine 1 mg/ml 1 ml                     

PLL       多聚赖氨酸       Poly-L-Lysine 10 mg/ml      1 ml                     

GSN      0.2％的明胶溶液      0.2% Gelatin Solution  100ml                         

FBS       胎牛血清    Fetal Bovine Serum, Human Cell Tested   500 ml                        

P/S  青霉素/链霉素溶液        Penicillin/Streptomycin Solution  5 ml                     

P/S  青霉素/链霉素溶液        Penicillin/Streptomycin Solution  100 ml                        

AMS      抗真菌溶液       Antimycotic Solution    50 ml                          

ABAMS       抗菌/抗霉菌溶液      Antibiotic/Antimycotic Solution  50 ml