【人β2糖蛋白(β2-GP)ELISA试剂盒现货】ELISA试剂盒重点介绍

 

ELISA酶联法试剂盒操作因固相载体的形成不同而有所差异，国内医学检验一般均用板式点。本文将叙述板式ELISA各个操作步骤的注意要点，珠式、管式及磁性球ELSIA，国外试剂均与特殊仪器配合应用，两者均有详细的使用说明，严格遵照规定操作，必能得出准确的结果。齐一生物销售：021－6034 8496；181214 53965；173021 04490

 

首先齐一生物优质的试剂盒，良好的仪器和正确的操作是保证ELISA检测结果准确可靠必要条件。所以ELISA试剂盒的购买，试剂质量的保证以及实验前实验仪器的检察都是很有重要性的。保证实验材料的质量之后要严格按照实验步骤进行。齐一生物销售：021－6034 8496；181214 53965；173021 04490

 

【人β2糖蛋白(β2-GP)ELISA试剂盒现货】齐一生物ELISA试剂盒

1.精心挑选的进口抗体、标准品、显色剂和酶标板全部为进口产品实现佳性能；

2.原材料批量进口加上自配的特殊配方稀释剂保证高品质产品性能的同时兼顾成本；

3.采用先进专利技术，严格按照标准生产方案组装生产；

4.预包被微孔板的批内和批间变异系数均小于10 ％；

5.经过严格交叉反应和干扰测试及稳定性试验；

6.明确的敏感度；

7.针对所有样品类型达到佳的性能；

8.广泛的检测范围可以满足大多数检测要求；

9.完善的售后服务和免费的技术支持免去您的后顾之忧；

10.强大的生产能力和充足的储备、操作熟练的技术人员及高效的生产流程，随时可以组装出您定制的产品。

【人β2糖蛋白(β2-GP)ELISA试剂盒现货】齐一生物仅供实验人、大鼠、小鼠、兔等动物实验标本检测使用！得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！齐一生物销售：021－6034 8496；181214 53965；173021 04490

 

ELISA试剂盒问题描述

标准曲线梯度差：

吸液或加液不准请检查移液器及吸头

标准品稀释不正确溶解标准品稍微旋转瓶身，轻轻混匀使粉末完全溶解

洗涤不完全保证洗涤时间和洗涤次数及每孔的加液量

显色很弱或无色：

孵育时间太短保证充足的孵育时间

实验温度不正确使用推荐的实验温度

试剂体积不够或漏加、稀释不正确检查吸液及加液过程，保证所有试剂按顺序足量添加

读数数值低：

酶标仪设置不正确 在酶标仪上检查波长及滤光片设置

提前打开酶标仪预热：变异系数大加液不正确检查加液情况

【人β2糖蛋白(β2-GP)ELISA试剂盒现货】背景值高：

检测抗体的工作浓度使用推荐的稀释倍数

酶标板洗涤不完全重新阅读操作手册，保证清洗完全；如果用自动洗板机，请检查所有的出口是否有堵塞

洗液有污染配制新鲜的洗液

灵敏度低：

ELISA试剂盒保存按说明书要求保存相关试剂

读数前未终止OD读数前应在每孔中加入终止液

 

齐一生物客户寄标本时需注明以下情况：

1、标本编号；2、所测项目；3、是否做复孔；3、联系方式；4、实验后标本是否寄回。

客户必知：

客户应对所提供的材料及信息负责，如因客户提供的材料及信息不准确而引起的实验延误或经济损失由客户承担。

 

试剂盒样本要求

在收集标本前都必须有一个完整的计划，必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测，对收集后当天就进行检测的标本，及时储存在4℃备用，如有特殊原因需要周期收集标本，请造模取材后，将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差，蛋白极易降解，直接影响实验质量，所以避免反复冻融。具体操作注意事项请与我司技术人员沟通。齐一生物销售：021－6034 8496；181214 53965；173021 04490

 

【人β2糖蛋白(β2-GP)ELISA试剂盒现货】液体类标本：标本必须为液体，不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。

1. 血清：室温血液自然凝固10-20分钟后，离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。

2. 血浆：应根据标本的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂，混合10-20分钟后，离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成应再次离心。

3. 尿液、胸腹水、脑脊液：用无菌管收集。离心20分钟左右（2000-3000转/分）。仔细收集上清。如有沉淀形成，应再次离心。

4. 细胞培养上清：检测分泌性的成份时，用无菌管收集。离心20分钟左右（2000-3000转/分）。仔细收集上清。

5. 培养细胞:检测细胞内的成份时，用PBS（PH7.2-7.4）稀释细胞悬液，细胞浓度达到100万/ml左右。通过反复冻融或加入组织蛋白萃取试剂，以使细胞破坏并放出细胞内成份。离心20分钟左右（2000-3000转/分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。

6. 组织标本：切割标本后，称取重量。加入一定量的PBS，缓冲液中可加入1μg/L蛋白酶抑制剂或50U/ml的Aprotinin（抑肽酶）。用手工或匀浆器将标本匀浆充分。离心20分钟左右（2000-3000转/分）。仔细收集上清置于-20度或-70度保存，如有必要，可以将样品浓缩干燥。分装后一份待检测，其余冷冻备用。

7. 标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融

8.不能检测含NaN3的样品，因NaN3抑制辣根过氧化物酶的（HRP）活性。

9.如果样品不能立即检测，应将其分装，-70 ℃保存，避免反复冷冻。保存过程中如出现沉淀，应再次离心。血液标本尽可能的不要使用溶血或高血脂血。如果血清中含大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解

冻。应在室温下解冻并确保样品均匀地充分解冻。

9241770  QIAxtractor, Premium Agreement The Premium Cover Agreement, QIAxtractor provides full service coverage for the QIAxtractor ａnd the CAS 1280. Costs for service visits are fully covered including travel, labor, ａnd parts. One on-site pipetting head service ａnd inspection is included. With a Premium Cover Agreement, there is a priority response time of 24 hours (1 working day).       CNY                           

9238971  Target Prep, Full Agreement  The Full Agreement TargetPrep provides full service coverage for the BioRobot TargetPrep. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).       CNY                           

9238953  Vertical plate, BS15        CNY                           

9238792  Angle rotor for 12 Falcon tubes15 ml          CNY                           

9017118  LiquiChip IS 200, Plate Handler            CNY                           

9232556  Vacuum manifold, strip holder 1.02             CNY                           

9232477  Lid, wash station, BR8000 v1.01          CNY                            【人β2糖蛋白(β2-GP)ELISA试剂盒现货】

9238940  PCB, CSU-02, BS15             CNY                           

9238783  Swing-out rotor for 4 buckets      Sigma Rotor # 11150     CNY                           

9238914  Shield motor, BS96        CNY                           

9238705  MP-motor capacitor             CNY                           

9231890  Base, 1-8 connector, BR8000        CNY                           

9238688  PC board Zent1, no Eprom, parameter free         CNY                           

9231133  Flat Band Cable 10P 1170mm Xmain-Xadp          CNY                           

9020069  Front plate, TLyser 2            CNY                           

9020058  Fuse T 0,63A, 220-240V 50/60Hz, TLyser          CNY                           

9014867  Valve, Pinch, RoboVac, BR8         CNY                           

9014599  PCB, YZ-Adapter, BR8          CNY                           

9014579  QIAVac Multiwell    Vacuum manifold for processing 96-well purification plates of SBS standard     CNY                           

9022581  PCB - Mainboard(progr.)Biocontrol,RGQII         CNY                           

9022409  RCS Tip Eject Station v.2             CNY                           

9022130  Optical Head Assembly, careHPV         CNY                           

9022125  Tool, Thermistor Gauge, RGx              CNY                            【人β2糖蛋白(β2-GP)ELISA试剂盒现货】

9021983  Latching mechanism (2), top lids, QXA              CNY                           

9021290  Silicone grease for PM, EZ1         CNY                           

9238617  Magnet, tacho        CNY                           

9238473  Rotary flex circuit, BR RapidPlate        CNY                           

9238240  BioRobot M48 User Manual          CNY                           

9238225  Sheath Delivery Tubing w. Connectors        CNY                           

9238158  Heat Transfer Adapter, microplate FB          CNY                           

9240660  Block, Plastic, QC, AP96/196              CNY                           

CCGW    细胞培养超纯水   Cell Culture Grade Water       500 ml                        

MDS     肌细胞分离液       Myocyte Detachment Solution      100 ml                         【人β2糖蛋白(β2-GP)ELISA试剂盒现货】

BPE 牛脑垂体提取物   Bovine Pituitary Extract  25 mg                         

BPE 牛脑垂体提取物   Bovine Pituitary Extract  100 mg                       

ITS  胰岛素铁硒传递蛋白    Insulin-Transferrin-Selenium, 100x      10 ml                          

L-Glu      左旋谷氨酰胺溶液      L-Glutamine Solution, 200 mM     100 ml                        

NEAA     100X非必需氨基酸      100X Non-Essential Amino Acids  100ml                         

HGL PCR       人胚层检测试剂盒       Human Germ Layer Detection Kit  50 reactions                       

Ored       油红O染色试剂盒       Oil Red O Staining Kit    100 ml                        

StemDS  人胚胎干细胞分离液    StemDS® Human Embryonic Stem Cell Dissociation Solution    100 ml                        

BPF 牛血浆纤维粘连蛋白    Bovine Plasma Fibronectin     1 mg                           

HCF 人细胞纤连蛋白    Human Cellular Fibronectin    100 μg                      

BPV 牛血浆玻连蛋白    Bovine Plasma Vitronectin    100 μg                      

rHSA      重组人血清白蛋白       "Recombinant Human Serum Albumin

"      1g                        

BPF 牛血浆纤维粘连蛋白    Bovine Plasma Fibronectin     1 mg                           

CCCSCK 胶原I-细胞培养表面包被试剂盒      Collage I-Cell Culture Surface Coating Kit    10 mg                         

PLL 多聚赖氨酸   Poly-L-Lysine 1 mg/ml   1 ml                     

PLL 多聚赖氨酸   Poly-L-Lysine 10 mg/ml 1 ml                      【人β2糖蛋白(β2-GP)ELISA试剂盒现货】

STEMium       人胚胎干细胞无血清培养基       Human Pluripotent Stem Cell Growth Medium     500 ml                        

STEMium-XF 无异种人胚胎干细胞生长培养基       Xeno-free Human Pluripotent Stem Cell Growth Medium    500 ml                        

STEMium-ACF      无动物成分的人胚胎干细胞生长培养基    Animal Component-free Human Pluripotent Stem Cell Growth Medium   500 ml                        

StemDS  人胚胎干细胞分离液    StemDS® Human Embryonic Stem Cell Dissociation Solution    100 ml                        

StemCryo       人胚胎干细胞冻存液    Human Pluripotent Stem Cell Cryopreservation Medium      50ml                           

StemCryo       人胚胎干细胞冻存液    Human Pluripotent Stem Cell Cryopreservation Medium,     5 x 10mL                           

MEF       小鼠胚胎成纤维细胞    Mouse Embryonic Fibroblasts       5 x 10^5 cells/vial                       

MEF-mt  小鼠胚胎成纤维细胞－－处理 Mouse Embryonic Fibroblasts-mitomicine treated       5 x 10^5 cells/vial                       

MEF-mt  小鼠胚胎成纤维细胞－－CD1小鼠C处理    Mouse Embryonic Fibroblasts- Mitomycin C treated from CD1 mouse     1 x 10^6 cells/vial                     

MEF-mt  小鼠胚胎成纤维细胞－－CF1小鼠C处理     Mouse Embryonic Fibroblasts from CF1 mouse 1 x 10^6 cells/vial                       

MEF-mt  小鼠胚胎成纤维细胞－－CF1小鼠C处理     Mouse Embryonic Fibroblasts-Mitomycin C treated from CF1 mouse      1 x 10^6 cells/vial                     

HDF-f-mt       人真皮成纤维细胞-胎儿C处理  Human Dermal Fibroblasts-Fetal-Mitomycin C treated 1 x 10^6 cells/vial                      

MEF-cm 小鼠胚胎成纤维细胞培养基       Mouse Embryonic Fibroblasts-Conditioned Medium    100 ml                        

bFGF-std MEF-cm 碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基       bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium   100ml                         

ULBC plates    干细胞超低结合培养板       Ultra-Low Binding Culture Plate    6-well, 1 plate                          

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5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9237124       Upgrade App., MinElute 96       Take advantage of our instrumentation ａnd molecular biology expertise to install MinElute 96  on your BioRobot System.  The onsite protocol development visit includes all travel ａnd labor for the visit to install the standard QIAGEN MinElute 96 protocol, plus a MinElute 96 BioRobot Kit (4-plate) Cat # 28051 CNY                           

9237099       Upgrade BR configuration, on site            CNY                          

9236424       Motor, rotary, TWI             CNY                          

9232970       YZ 96 Connector Board 2 (Arm), E10103            CNY                          

9236409       Plate Stacker TW I, microplate         CNY                          

9236357       Sample Holder Large, 1.5ml             CNY                           【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

9232911       Board, YZ driver         CNY                          

9236336       Analog Board              CNY                          

9236158       Pot. , syr. or vert. , kit, RapidPlate           CNY                          

9236149       Locator Plate, 96 well,pos 4-5,BR RP            CNY                          

9236114       O-Ring, Coupling, RoboVac, BR8 RD v4.2           CNY                          

9236107       Flange Tool          CNY                          

9230680       Probe, 0.4 mm, BR8000 v1.01         CNY                          

9230478       Rack f. Diamend Gel Cards (holds 6cards)            CNY                          

9230473       Set, Cables, PCB (X-main) to shaker             CNY                          

9235646       Connector, Board, HS shaker, BR8000 v1.0         CNY                          

9235255       T-piece, HSPS tubing, connector             CNY                          

9235190       Motor, clamp, shaker plate,  BR8000           CNY                          

9230060       Rack for reagents (12 x diameter 21mm)         CNY                          

9238673       Hinge, imbalance system            CNY                          

9238653       Power Board, temp. switch - heat sink            CNY                          

9238635       Hook identification compl.         CNY                           【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

9242028       RGQ, Repair Fee "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"    CNY                          

9241821       Pyro Q96 MDa, Basic Agreement    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"  CNY                          

9241786       QIAgility Demopool Box Light Version           CNY                          

9240976       PCB, display, sample tracking, Qsym              CNY                          

9240968       Barcode camera,  sample tracking, Qsym             CNY                          

9240816       #Full Agreement, EZ1 DSP, 1YR             CNY                          

9240680       Spill Tray, Shaker,Modified, AP        CNY                          

9240790       Update,Waste Bottle Sensor, AP196/98         CNY                          

6000-5014    Lumicheck Plate Service Package, DML2             CNY                          

TGS       滋养层细胞生长因子     Trophoblast Growth Supplement       5 ml                     

PAdDS  前脂肪细胞分化生长因子     Preadipocyte Differentiation Supplement                             

PAGS    前脂肪细胞生长因子     Preadipocyte Growth Supplement     5 ml                      【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

AdGS    脂肪细胞生长因子   Adipocyte Growth Supplement  5 ml                     

OEpiCGS     卵巢上皮细胞生长因子  Ovarian Epithelial Cell Growth Supplement     5 ml                     

MODS   间充质干细胞成骨分化生长因子       Mesenchymal Stem Cell Osteogenic Differentiation supplement    5 ml                     

MADS   间充质干细胞脂肪分化生长因子       Mesenchymal Stem Cell Adipogenic Differentiation supplement    5 ml                     

MSCGS 间充质干细胞生长因子  Mesenchymal Stem Cell Growth Supplement  5 ml                     

MSCGS-sf    间充质干细胞生长因子  Mesenchymal Stem Cell Growth Supplement-serum free      5 ml                     

MSCGS-acf  间充质干细胞生长因子-无动物组分  Mesenchymal Stem Cell Growth Supplement-animal component free 5 ml                     

MCDS   间充质干细胞软骨分化生长因子       Mesenchymal Stem Cell Chondrogenic Differentiation Supplement      5 ml                        

MEpiCGS     乳腺上皮细胞生长因子       Mammary Epithelial Cell Growth Supplement  5 ml                     

FBS       胎牛血清     Fetal Bovine Serum, Human Cell Tested   10 ml                          

FBS       胎牛血清     Fetal Bovine Serum, Human Cell Tested   25 ml                          

T/E  胰酶/EDTA消化液   Trypsin/EDTA solution 100 ml                        

TNS       胰酶中和液 Trypsin Neutralization Solution   100 ml                        

TNS-sf 胰酶中和液-无血清  TNS-sf (Trypsin Neutralization Solution-serum free)      100 ml                        

ECDS    非酶细胞裂解液       Enzyme-Free Cell Dissociation Solution   100 ml                        

CFM      细胞冻存液 Cell Freezing Medium  50 ml                           【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

SF-CFM       无血清细胞冻存液        Serum-Free Cell Freezing Medium    50 ml                          

StemCryo      人胚胎干细胞冻存液      Human Pluripotent Stem Cell Cryopreservation Medium       50ml                           

StemCryo      人胚胎干细胞冻存液      Human Pluripotent Stem Cell Cryopreservation Medium,      5 x 10mL                          

TB  台盼蓝  Trypan Blue, 0.4%       50 ml                          

DPBS    磷酸盐缓冲液   Dulbecco’s Phosphate-Buffered Saline  500 ml                        

HBSS    平衡盐溶液       Hank＇s Balanced Salt Solution    500 ml                        

PLL       多聚赖氨酸       Poly-L-Lysine 1 mg/ml 1 ml                     

PLL       多聚赖氨酸       Poly-L-Lysine 10 mg/ml      1 ml                     

GSN      0.2％的明胶溶液      0.2% Gelatin Solution  100ml                         

FBS       胎牛血清    Fetal Bovine Serum, Human Cell Tested   500 ml                        

P/S  青霉素/链霉素溶液        Penicillin/Streptomycin Solution  5 ml                     

P/S  青霉素/链霉素溶液        Penicillin/Streptomycin Solution  100 ml                        

AMS      抗真菌溶液       Antimycotic Solution    50 ml                          

ABAMS       抗菌/抗霉菌溶液      Antibiotic/Antimycotic Solution  50 ml