【人α乳清蛋白(α-La)ELISA试剂盒厂家】ELISA 样本的处理

ELISA 检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。齐一生物销售：021－6034 8496；181214 53965；173021 04490

 

注意事项：

◆   每个标本量收集体积＝  100ul × 检测种类，如要做复孔，标本量收集体积＝  100ul × 检测种类×2 。

◆   对于作某一个具体指标来说，先用一系列稀释度作一批标本测定，得出对实验有意义的一个稀释度（即测定剂量反应曲线），然后用一个适稀释度测定即可。

◆   收集标本前必须清楚要检测的成份是否足够稳定。对收集后当天进行检测的标本，储存在4℃备用，如有特殊原因需要周期收集标本，将标本及时分装后放在-20℃ 或-70℃ 条件下保存。避免反复冻融。标本 2 -8 ℃ 可保存48 小时.-20℃ 可保存1个月。 -70 度可保存6个月。部分激素类标本需添加抑肽酶。

◆   血清标本采集时，应注意避免溶血，红细胞溶解时会释放出具有过氧化物酶活性的物质，以 HRP为标记的 ELISA 测定中，溶血标本可能会增加非特异性显色。

【人α乳清蛋白(α-La)ELISA试剂盒厂家】◆   为了保证尿液检测结果的准确性，必须正确收集尿液标本和保存。盛尿容器要清洁干燥。使用一次性的容器（如塑料尿杯），避免因用药并清洗不干净而造成的污染，影响检测结果。尿液标本必须新鲜，留取后，应及时检测或保存，以免细菌繁殖。因在室温中久置后（尤其是夏季）尿中磷酸盐等可析出结晶而干扰检测。

◆    标本宜在新鲜时检测。如有细菌污染，菌体中可能含有内源性 HRP，也会产生假阳性反应。保存过久可发生聚合在 ELISA中可使本底加深。

◆   冻结标本融解后，蛋白质局部浓缩，分布不均，应充分混匀宜轻缓，避免气泡，可上下颠倒混和，不要在混匀器上强烈振荡。

◆   混浊或有沉淀的标本应先离心或过滤，澄清后再检测。

◆   反复冻融会使蛋白效价降低，所以待测标本如需保存作多次检测，宜少量分装冰存。也可加入适当防腐剂。

◆   如果您在实验中遇到任何特殊问题，敬请咨询 021-60348496标本类型

ELISA常见的标本一般包括：

◆   液体类标本：包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清等。

◆   培养细胞

◆   组织标本

这些标本收集的时间、方法和保存都有一定的要求。

【人α乳清蛋白(α-La)ELISA试剂盒厂家】标本的保存

一般说来，在 5 天内测定的标本可放置于 4℃，超过一周测定的需低温冻存。

对收集后当天进行检测的标本，储存在  4 ℃ 备用，如有特殊原因需要周期收集标本， 将标本及时分装后放在  -20 ℃ 或  -70 ℃ 条件下保存。避免反复冻融。 标本  2 -8 ℃ 可保存  48 小时，-20 ℃ 可保存  1 个月。-70 度可保存  6 个月。部分标本也可加入适当防腐剂，激素类标本需添加抑肽酶。标本的处理 可用作 ELISA 测定的标本十分广泛，体液（如血清）、分泌物（唾液）和排泄物（如尿液）、细胞培养上清、细胞、组织等均可作标本以测定其中某种成份。有些标本可直接进行测定，有些则需经预处理。

◆ 血清：  

室温血液自然凝固  10-20 分钟后，离心  20 分钟左右（  2000-3000 转  / 分）。收集上清。如有沉淀形成，应再次离心。  

◆ 血浆：  

应根据试剂盒的要求选择  EDTA 、柠檬酸钠或肝素作为抗凝剂，加入  10 ％（  v/v ）抗凝剂 ( 0.1M 柠檬酸钠或  1% heparin 或  2.0%EDTA.Na2) 混合  10-20 分钟后，离心  20 分钟左右（  2000-3000 转  / 分）。仔细收集上清如有沉淀形 成，应再次离心。  

【人α乳清蛋白(α-La)ELISA试剂盒厂家】◆ 尿液、胸腹水、脑脊液：  

用无菌管收集。离心  20 分钟左右（  2000-3000 转  / 分）。仔细收集上清。如有沉淀形成，应再次离心。  

◆ 细胞培养上清：  

检测分泌性的成份时，用无菌管收集。离心  20 分钟左右（  2000-3000 转  / 分）。仔细收集上清。检测细胞内的 成份时，用  PBS （  PH7.2-7.4 ）稀释细胞悬液，细胞浓度达到  100 万 /ml 左右。通过反复冻融，以使细胞破坏并放出细胞内成份。离心  20 分钟左右（  2000-3000 转 / 分）。仔细收集上清。保存过程中如有沉淀形成，应再次离心。  

◆ 细胞：

检测细胞的成份时，对于贴壁细胞，去除培养液，用PBS、生理盐水或无血清培养液洗一遍。加入适量裂解液。用枪吹打数下，使裂解液和细胞充分接触。通常裂解液接触细胞10 秒后，细胞就会被裂解。对于悬浮细胞，离心收集细胞，用PBS、生理盐水或无血清培养液洗一遍.加入适量裂解液, 用枪吹打把细胞吹散。用手指轻弹以充分裂解细胞。充分裂解后应没有明显的细胞沉淀。如果细胞量较多，必需分装然后再裂解。充分裂解后，10000-14000g离心3-5 分钟，取上

清，即可进行后续操作。

 

【人α乳清蛋白(α-La)ELISA试剂盒厂家】◆ 组织标本：  

切割标本后，称取重量。加入一定量的  PBS或组织蛋白萃取试剂，缓冲液中可加入蛋白酶抑制剂。用手工或匀浆器将标本匀浆充分。离心  20 分钟左右（  2000-3000 转  / 分）。仔细收集上清置于  -20 度或  - 70 度保存，如有必要，可以将样品浓缩干燥。分装后一份待检测，其余冷冻备用。齐一生物销售：021－6034 8496；181214 53965；173021 04490  

9240651  Internal BCR, Keyence, BL-180, AP96/196          CNY                           

9240626  Fuse, 8amp, 5 X 20 mm, AP196/98             CNY                           

9240623  Circuit Breaker, 2A, AP196/98             CNY                           

9240591  3-Way Mac Valve, AP196/96         CNY                           

9240574  Micro-Lynx w\speedbaord, AP98         CNY                           

9240572  Rotor w/Buckets, Sorvall, AP96           CNY                           

9240552  Freq. Conv., PCB, Eppendorf, AP196/98             CNY                           

9240531  Cable, Motor, Axis #7, Pump U/D, AP98             CNY                            【人α乳清蛋白(α-La)ELISA试剂盒厂家】

9240564  Fan, Condenser, 230V,Eppendorf, AP196/98        CNY                           

9240553  Cable, PC-104 to Eppendorf, AP196/98              CNY                           

9240548  Motor Cap Repair, Eppendorf, AP196/98             CNY                           

9240515  I/O PCB, Main Board, AP196/98          CNY                           

9240503  PM Kit, AP196              CNY                           

9239958  BR M48, PM Service     "Service response time (repair): not applicable

 

Periodic inspection/maintenance: yes

 Inspection/maintenance delivery: onsite

 

Cost coverage for Repair Parts : not included

 

Cost coverage for labor: yes, full coverage

 

Cost coverage for travel: yes, full coverage

 

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9239853  Foot, complete, v2.0 (4)              CNY                           

9239572  BR Universal, Full Agreement The Full Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).       CNY                           

9239563  Assy,Kit,Retrofit,HoriCompressorMnt          CNY                           

9020033  LED UV (325), RGQ            CNY                           

9020023  L 26_7 Firmware, DML2             CNY                           

9019975  PCB, UV light assembly, Cxx        CNY                           

9238858  Basic Cover Agreement Plato7, 1yr       The Basic Cover Agreement Plato7 provides full service coverage for the Plato 7 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9241233  User Manual, QIAxcel FA 01/08 (WW)        CNY                           

9241227  ZLV, Handscanner, Qsym             CNY                           

9241180  Motor, Z, QX         CNY                            【人α乳清蛋白(α-La)ELISA试剂盒厂家】

9019473  Loading guide, 52 well sharkstooth, GSC            CNY                           

9019462  Switch, micro, HS         CNY                           

9019457  Fans (2), 5V, 30mm, HS              CNY                           

9237172  Cable USB             CNY                           

9233169  Board, YZ-CPU, BR8000              CNY                           

9237163  Bezel LiquiChip             CNY                           

9237162  Shipping Plug XYP        CNY                           

ULBC plates    超低结合培养板（6孔）     Ultra-Low Binding Culture Plate (6-well)     "6-well, 4 plates

"                           【人α乳清蛋白(α-La)ELISA试剂盒厂家】

ULBC plate     超低结合培养板（24孔）   Ultra-Low Binding Culture Plate (24-well)   "24-well, 1 plate

"                          

ULBC plates    超低结合培养板（24孔）   Ultra-Low Binding Culture Plate (24-well)   "24-well, 4 plates

"                          

rhbFGF   重组人碱性成纤维细胞生长因子－－碱性     Recombinant Human Fibroblast Growth Factor-basic  10µg                           

rhEGF     重组人表皮生长因子    "Recombinant Human Epidermal Growth Factor

"      100µg                         

rhBMP-2 重组人骨形成蛋白-2    "Recombinant Human Bone Morphogenetic Protein-2

"      2µg                      

rhBMP-4 重组人骨形成蛋白-4    "Recombinant Human Bone Morphogenetic Protein-4

"      2µg                      

rhBMP-7 重组人骨形成蛋白-7    "Recombinant Human Bone Morphogenetic Protein-7

"      2µg                      

rhNOGGIN    重组人NOGGIN蛋白  "Recombinant Human NOGGIN

"      5µg                      

HAMSC  人羊膜间充质基质细胞       Human Amniotic Mesenchymal Stromal Cells      5 x 10^5 cells/vial                       

HCMSC  人绒毛膜间充质基质细胞    Human Chorionic Mesenchymal Stromal Cells    5 x 10^5 cells/vial                       

HMSC-bm      人间充质干细胞－－骨髓 Human Mesenchymal Stem Cells-bone marrow   5 x 10^5 cells/vial                       

HMSC-ad       人间充质干细胞－－脂肪 Human Mesenchymal Stem Cells-adipose     5 x 10^5 cells/vial                       

HMSC-hp       人间充质干细胞－－肝脏 Human Mesenchymal Stem Cells-hepatic     5 x 10^5 cells/vial                       

HUMSC  人脐带间充质干细胞    Human Umbilical Mesenchymal Stem Cells   5 x 10^5 cells/vial                       

HPMSC   人肺间充质干细胞       Human Pulmonary Mesenchymal Stem Cells       5 x 10^5 cells/vial                       

HVMSC  人脊椎间充质干细胞    Human Vertebral Mesenchymal Stem Cells   5 x 10^5 cells/vial                        【人α乳清蛋白(α-La)ELISA试剂盒厂家】

MSCM    间充质干细胞培养基  Mesenchymal Stem Cell Medium   500 ml                        

MODM-500    间充质干细胞-成骨细胞分化培养基 Mesenchymal Stem Cell Oseogenic Differentiation Medium 500 ml                        

MODM-250           Mesenchymal Stem Cell Oseogenic Differentiation Medium 250 ml                        

MODM-b      间充质干细胞-成骨细胞分化培养基-基础 Mesenchymal Stem Cell Osteogenic Differentiation Medium-basal        500ml                         

MODM-b-prf        间充质干细胞-成骨细胞分化培养基-无酚红     Mesenchymal Stem Cell Osteogenic Differentiation Medium-basal-phenol red free      500ml                         

MADM-500    间充质干细胞-脂肪细胞分化培养基  Mesenchymal Stem Cell Adipogenic Differentiation Medium       500 ml                        

MADM-250           Mesenchymal Stem Cell Adipogenic Differentiation Medium       250 ml                        

MADM-b       间充质干细胞-脂肪细胞分化培养基-基础 Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal        500 ml                        

MADM-b-prf        间充质干细胞-脂肪细胞分化培养基-无酚红     Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal-phenol red free       500ml                         

MCDM   间充质干细胞-软骨分化培养基  Mesenchymal Stem Cell Chondrogenic Differentiation Medium   500ml                         

Ared       茜素红S染色试剂盒    Alizarin Red S Staining Kit     100 ml                        

Ored       油红O染色试剂盒       Oil Red O Staining Kit    100 ml                        

PCR 人间质干细胞成脂肪细胞检测试剂盒       Human Mesenchymal Stem Cell Adipogenesis Detection Kit       50 reactions                        

HMSC-C PCR 人间充质干细胞软骨形成检测试剂盒       Human Mesenchymal Stem Cell Chondrogenesis Detection Kit   50 reactions                            

SafraninO       番红O染色试剂盒       Safranin O Staining Kit   100 reactions                            

ABlue      阿利新蓝染色试剂盒    Alcian Blue Staining Kit  100 reactions                             【人α乳清蛋白(α-La)ELISA试剂盒厂家】

s�ci�*�)ily:"Times New Roman";Times New Roman"＇>人真皮成纤维细胞-胎儿C处理  Human Dermal Fibroblasts-Fetal-Mitomycin C treated 1 x 10^6 cells/vial                      

 

MEF-cm 小鼠胚胎成纤维细胞培养基       Mouse Embryonic Fibroblasts-Conditioned Medium    100 ml                        

bFGF-std MEF-cm 碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基       bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium   100ml                         

ULBC plates    干细胞超低结合培养板       Ultra-Low Binding Culture Plate    6-well, 1 plate                          

l�K���*�)si-font-family:"Times New Roman";color:black;

 

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9237124       Upgrade App., MinElute 96       Take advantage of our instrumentation ａnd molecular biology expertise to install MinElute 96  on your BioRobot System.  The onsite protocol development visit includes all travel ａnd labor for the visit to install the standard QIAGEN MinElute 96 protocol, plus a MinElute 96 BioRobot Kit (4-plate) Cat # 28051 CNY                           

9237099       Upgrade BR configuration, on site            CNY                          

9236424       Motor, rotary, TWI             CNY                          

9232970       YZ 96 Connector Board 2 (Arm), E10103            CNY                          

9236409       Plate Stacker TW I, microplate         CNY                          

9236357       Sample Holder Large, 1.5ml             CNY                           【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

9232911       Board, YZ driver         CNY                          

9236336       Analog Board              CNY                          

9236158       Pot. , syr. or vert. , kit, RapidPlate           CNY                          

9236149       Locator Plate, 96 well,pos 4-5,BR RP            CNY                          

9236114       O-Ring, Coupling, RoboVac, BR8 RD v4.2           CNY                          

9236107       Flange Tool          CNY                          

9230680       Probe, 0.4 mm, BR8000 v1.01         CNY                          

9230478       Rack f. Diamend Gel Cards (holds 6cards)            CNY                          

9230473       Set, Cables, PCB (X-main) to shaker             CNY                          

9235646       Connector, Board, HS shaker, BR8000 v1.0         CNY                          

9235255       T-piece, HSPS tubing, connector             CNY                          

9235190       Motor, clamp, shaker plate,  BR8000           CNY                          

9230060       Rack for reagents (12 x diameter 21mm)         CNY                          

9238673       Hinge, imbalance system            CNY                          

9238653       Power Board, temp. switch - heat sink            CNY                          

9238635       Hook identification compl.         CNY                           【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

9242028       RGQ, Repair Fee "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"    CNY                          

9241821       Pyro Q96 MDa, Basic Agreement    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"  CNY                          

9241786       QIAgility Demopool Box Light Version           CNY                          

9240976       PCB, display, sample tracking, Qsym              CNY                          

9240968       Barcode camera,  sample tracking, Qsym             CNY                          

9240816       #Full Agreement, EZ1 DSP, 1YR             CNY                          

9240680       Spill Tray, Shaker,Modified, AP        CNY                          

9240790       Update,Waste Bottle Sensor, AP196/98         CNY                          

6000-5014    Lumicheck Plate Service Package, DML2             CNY                          

TGS       滋养层细胞生长因子     Trophoblast Growth Supplement       5 ml                     

PAdDS  前脂肪细胞分化生长因子     Preadipocyte Differentiation Supplement                             

PAGS    前脂肪细胞生长因子     Preadipocyte Growth Supplement     5 ml                      【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

AdGS    脂肪细胞生长因子   Adipocyte Growth Supplement  5 ml                     

OEpiCGS     卵巢上皮细胞生长因子  Ovarian Epithelial Cell Growth Supplement     5 ml                     

MODS   间充质干细胞成骨分化生长因子       Mesenchymal Stem Cell Osteogenic Differentiation supplement    5 ml                     

MADS   间充质干细胞脂肪分化生长因子       Mesenchymal Stem Cell Adipogenic Differentiation supplement    5 ml                     

MSCGS 间充质干细胞生长因子  Mesenchymal Stem Cell Growth Supplement  5 ml                     

MSCGS-sf    间充质干细胞生长因子  Mesenchymal Stem Cell Growth Supplement-serum free      5 ml                     

MSCGS-acf  间充质干细胞生长因子-无动物组分  Mesenchymal Stem Cell Growth Supplement-animal component free 5 ml                     

MCDS   间充质干细胞软骨分化生长因子       Mesenchymal Stem Cell Chondrogenic Differentiation Supplement      5 ml                        

MEpiCGS     乳腺上皮细胞生长因子       Mammary Epithelial Cell Growth Supplement  5 ml                     

FBS       胎牛血清     Fetal Bovine Serum, Human Cell Tested   10 ml                          

FBS       胎牛血清     Fetal Bovine Serum, Human Cell Tested   25 ml                          

T/E  胰酶/EDTA消化液   Trypsin/EDTA solution 100 ml                        

TNS       胰酶中和液 Trypsin Neutralization Solution   100 ml                        

TNS-sf 胰酶中和液-无血清  TNS-sf (Trypsin Neutralization Solution-serum free)      100 ml                        

ECDS    非酶细胞裂解液       Enzyme-Free Cell Dissociation Solution   100 ml                        

CFM      细胞冻存液 Cell Freezing Medium  50 ml                           【人淀粉样前体蛋白(βAPP)ELISA试剂盒厂家】

SF-CFM       无血清细胞冻存液        Serum-Free Cell Freezing Medium    50 ml                          

StemCryo      人胚胎干细胞冻存液      Human Pluripotent Stem Cell Cryopreservation Medium       50ml                           

StemCryo      人胚胎干细胞冻存液      Human Pluripotent Stem Cell Cryopreservation Medium,      5 x 10mL                          

TB  台盼蓝  Trypan Blue, 0.4%       50 ml                          

DPBS    磷酸盐缓冲液   Dulbecco’s Phosphate-Buffered Saline  500 ml                        

HBSS    平衡盐溶液       Hank＇s Balanced Salt Solution    500 ml                        

PLL       多聚赖氨酸       Poly-L-Lysine 1 mg/ml 1 ml                     

PLL       多聚赖氨酸       Poly-L-Lysine 10 mg/ml      1 ml                     

GSN      0.2％的明胶溶液      0.2% Gelatin Solution  100ml                         

FBS       胎牛血清    Fetal Bovine Serum, Human Cell Tested   500 ml                        

P/S  青霉素/链霉素溶液        Penicillin/Streptomycin Solution  5 ml                     

P/S  青霉素/链霉素溶液        Penicillin/Streptomycin Solution  100 ml                        

AMS      抗真菌溶液       Antimycotic Solution    50 ml                          

ABAMS       抗菌/抗霉菌溶液      Antibiotic/Antimycotic Solution  50 ml