【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人水通道蛋白4（AQP-4-AB）抗体elisa现货】样本保存条件：

1.2-8℃冰箱多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人水通道蛋白4（AQP-4-AB）抗体elisa现货】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人水通道蛋白4（AQP-4-AB）抗体elisa现货】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人水通道蛋白4（AQP-4-AB）抗体elisa现货】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

9240780  500 ml Reagent #6 Update, AP196        CNY                           

9240772  Assy, Sub, Dispense Head, AP96          CNY                           

9240769  Mono Touch Screen, AP96           CNY                           

9241465  IQ/OQ Services, QIAcube     Instrument qualification support service for the QIAcube. The product contains the IQ/OQ documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included).       CNY                           

9243647  Acceler. Valid Supp, QuantiPlex/QP Hyres    The QIAGEN Quantiplex/Quantiplex HYres Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introductory seminar about the Quantiplex/Quantiplex HYres quantification chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user reaction setup, ａnd best practices.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through Quantiplex/Quantiplex HYres validation, as well as general wet lab support such as pipetting for quantification to produce the data needed for the validation study. The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  CNY                           

9243646  Guided Valid Support, Other Quant. Sys       The QIAGEN Guided Validation Support, Other Quantification System service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide hands-on user reaction setup, ａnd best practices.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to validate (Plexor HY, Quantifiler Human, Quantifiler Y, Quantifiler Duo)  CNY                           

9243541  #Rapid Plate, PM Service      "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                            【人水通道蛋白4（AQP-4-AB）抗体elisa现货】

9243115  Lee Sample Valve, LC           CNY                           

9242863  QIArent&go - Rent out fee QSAS Cabinet           CNY                           

9242771  Tool, align encoder, Qsym            CNY                           

9242717  Validation Support, 5d, QIAcube   The QIAGEN QIAcube Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the QIAcube system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user support ａnd QIAcube instrument operation. The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.).       CNY                           

9242204  Adapter, 2x Rotor-Disc, Qsym             CNY                           

5000-1020R    DML 2000 Luminometer, 240V (Refurbished)            CNY                           

5000-1323      Rotary Shaker - Cord Line, 220V         CNY                           

5000-1317      Knob Speed Timer, SKR1             CNY                           

5000-1272      X Drive Track, DML2          CNY                           

5000-1260      Stepper Model New Styl, DML2          CNY                           

5001-2240      Hybrid Capture 2 (hc2)System Core, 240V         CNY                           

5000-1506      Switch ON-OFF 240V, HTR1              CNY                           

6000-4111ENV2.2  RCS User Application Manual (English)        CNY                           

6000-3117      Plate Washer I - Maintenance Kit          CNY                           

5199-1220      hc2 High-Risk HPV DNA Test             CNY                           

5126-1220      hc2 DNA Collection Device          CNY                            【人水通道蛋白4（AQP-4-AB）抗体elisa现货】

GR8RNA QIAshredder (250)/RNeasy (250) spezial            CNY                           

RNEZ4U QIAshredder (50)/RNeasy (50) special        CNY                           

9240736  Decon tube, Actril, AP98              CNY                           

5000-1410      Strip Shock Absorbing, VTX2             CNY                           

9240692  Fan, 12Vdc, PC-104 cover, AP196/98         CNY                           

51331     QIAamp 96 DNA QIAcube HT Kit (5)         CNY                           

74171     RNeasy 96 QIAcube HT Kit (5)           CNY                           

180994    GeneRead Adapter L Set 12-plex (72)          CNY                           

       └ 虹膜色素上皮细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 晶状体上皮细胞总DNA 1μg/1μl（10μg/20ug/50ug）                               【人水通道蛋白4（AQP-4-AB）抗体elisa现货】

       └ 角膜上皮细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 角膜内皮细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 视网膜神经节细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 淋巴内皮细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 淋巴成纤维细胞总DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 外周血白细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       肺大动脉内皮细胞       5 × 105       1ml                      

       肺大动脉平滑肌细胞    5 × 105       1ml                      

       肺大静脉内皮细胞       5 × 105       1ml                      

       肺大静脉平滑肌细胞    5 × 105       1ml                      

       肺动脉成纤维细胞       5 × 105       1ml                      

       肺泡上皮细胞       5 ×105  1ml                      

       气管上皮细胞       5 × 105       1ml                      

       肺微血管内皮细胞       5 × 105       1ml                      

       肺成纤维细胞       5 × 105       1ml                      

       心肌细胞       5 ×105  1ml                       【人水通道蛋白4（AQP-4-AB）抗体elisa现货】

       心肌成纤维细胞    5 × 105       1ml                      

       主动脉内皮细胞    5 × 105       1ml                      

       主动脉平滑肌细胞       5 × 105       1ml                      

       单核细胞       5 × 105       1ml                      

       大隐静脉平滑肌细胞    5 × 105       1ml                      

       食管上皮细胞       5 × 105       1ml                      

       食管平滑肌细胞    5 × 105       1ml                      

       肠动脉内皮细胞    5 × 105       1ml                      

       肠静脉内皮细胞    5 × 105       1ml                      

       肠平滑肌细胞       5 × 105       1ml                      

       肠粘膜上皮细胞    5 × 105       1ml                      

       肝实质细胞    5 ×105  1ml                      

       肝动脉内皮细胞    5 × 105       1ml                      

       肝动脉平滑肌细胞       5 × 105       1ml                      

       肝内胆管上皮细胞       5 × 105       1ml