检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人流感嗜血杆菌（HI）IGMELISA厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人流感嗜血杆菌（HI）IGMELISA厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人流感嗜血杆菌（HI）IGMELISA厂家】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【人流感嗜血杆菌（HI）IGMELISA厂家】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

9014794  Board, YZ6L, slave, BR8000              CNY                             

9014772  Card, X-main, bar code              CNY                             

9014686  Cable, Arm/East, BR8, RD                 CNY                             

9014535  RCS, Arm Cable, Flat, RCS1                CNY                             

9014497  Wash Station, complete, BR8 RD             CNY                             

9022475  Foam Inserts, Shipment, careHPV            CNY                              【人流感嗜血杆菌（HI）IGMELISA厂家】

9022273  PyroMark Q24 Plate Holder, PyroQ24               CNY                             

9021624  Kit, 8 ch pip. plunger upgrade, Cx8          CNY                             

9230198  Sealing ring, 2.5/1.5 mm           CNY                             

9238671  Cap, white                 CNY                             

9238651  Interference suppression filter F11.126/           CNY                             

9238641  Fan, power board ａnd casing  this is a 5.25" fan that is mounted horizontally underneath the centrifuge.  CNY                            

9238637  Diode BYW 35           CNY                             

9240970  Motor, x, sample tracking, Qsym              CNY                             

9240965  X-Ring, pipetting system, Qsym                 CNY                             

9240683  Spring, Elevator Pickup Head, AP196/98           CNY                             

9240814  #Agreement, EZ1 DSP, PM, 1YR                CNY                             

9240788  Update, Pump Pulley/Motor, AP98          CNY                             

6000-5008        RCS Reagent Troughs                CNY                             

6000-5004        Sealing Film Dispenser              CNY                             

9241768  QIAxtractor, Basic Agreement         The Basic Cover Agreement, QIAxtractor provides full service coverage for the QIAxtractor ａnd the CAS 1280. Costs for service visits are fully covered including travel, labor, ａnd parts. One on-site pipetting head service ａnd inspection is included. With a Basic Cover Agreement, there is a response time of 5 working days.   CNY                             

9241656  Transfer Rack Plate, AP             CNY                              【人流感嗜血杆菌（HI）IGMELISA厂家】

9241630  Shaker Adjustment Kit, AP                CNY                             

9241367  Damper, door, EZ1a                   CNY                             

9240759  Update, Sliding Door Latch, AP98             CNY                             

9243548  Pyro Q96 ID, PM Service "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9242766  Cogwheel, x-drive, portal, Qsym               CNY                             

9242710  Validation Support, 2d, QIAgility      The QIAGEN QIAgility Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the QIAgility system ａnd software, review the User Manual, ａnd practical hands-on operation. The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which processes (quant setup, normalization, PCR setup, CE setup) ａnd kit manufacturer (Applied Biosystems, Promega, QIAGEN) need to be included in the validation.     CNY                             

9242253  Cable, x, portal AS, Qsym                   CNY                             

9242234  RGQ, IQ/OQ Service         "Instrument qualification support service for the Rotorgene Q ａnd Rotorgene 6000. The product contains the IQ/OQ documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included).

During the service, the compliance with the manufacturer＇s installation requirements is testified ａnd the performance verification & dynamic calibration of the Rotorgene Q is performed." CNY                             

9242172  Repair fee, Depot Repair, TissueLyser II           CNY                             

QT-00180 阴沟肠杆菌ATCC13047 冻干粉                                         

QT-00181 表皮葡萄球菌CMCC(B)29069        冻干粉                                         

QT-00182 阪崎肠杆菌ATCC29544 冻干粉                                          【人流感嗜血杆菌（HI）IGMELISA厂家】

QT-00183 弗氏柠檬酸杆菌ATCC43864 冻干粉                                         

QT-00184 肺炎克雷伯氏菌ATCC10031 冻干粉                                         

QT-00185 小肠结肠炎耶尔森氏菌ATCC23715      冻干粉                                         

QT-00186 亚利桑那沙门氏菌CMCC(B)47001        冻干粉                                         

QT-00187 热带念珠菌ATCC13803 冻干粉                                         

QT-00188 克柔假丝酵母菌ATCC14243 冻干粉                                         

QT-00189 光滑念珠菌ATCC15126 冻干粉                                         

QT-00190 创伤弧菌ATCC27562      冻干粉                                         

QT-00191 婴儿双歧杆菌ATCC15697      冻干粉                                         

QT-00192 德式乳杆菌保加利亚亚种CICC6032     冻干粉                                         

QT-00193 生孢梭菌CMCC（B）64941  冻干粉                                         

QT-00194 嗜热链球菌CICC6038     冻干粉                                         

QT-00195 SphingobacteriumcompostiDSM18850   冻干粉                                         

QT-00196 猪霍乱沙门氏菌猪霍乱亚种DSM4224         冻干粉                                         

QT-00197 沼泽红假单胞菌DSM5859    冻干粉                                         

QT-00198 嗜盐细菌属DSMZ16465         冻干粉                                          【人流感嗜血杆菌（HI）IGMELISA厂家】

QT-00199 土壤伯克氏菌DSMZ18235    冻干粉                                         

QT-00200 烟酸芽孢杆菌DSMZ2923      冻干粉                                         

QT-00201 菲律宾链霉菌DSMZ40112=ATCC23905         冻干粉                                         

QT-00202 抗辐射链霉菌DSMZ41901    冻干粉                                         

QT-00203 短乳杆菌DSMZ6235      冻干粉                                         

QT-00204 乳酸片球菌JCM8791=DSM20284=NCIMB12174  冻干粉                                         

QT-00205 桃色欧文氏菌NBRC102418  冻干粉                                         

QT-00206 白色噬琼胶菌NBRC102603  冻干粉                                         

QT-00207 浮游球衣菌NBRC13543         冻干粉                                         

QT-00208 丁香假单胞菌NBRC14078     冻干粉                                         

QT-00209 睾丸酮丛毛单胞菌NBRC14951     冻干粉                                         

QT-00210 耐放射异常球菌NBRC15346         冻干粉                                         

QT-00211 烟酸芽孢杆菌NBRC15566     冻干粉                                         

QT-00212 新鞘氨醇单胞菌NBRC16725         冻干粉