检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人1,25二羟基维生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人1,25二羟基维生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人1,25二羟基维生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA试剂盒厂家】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【人1,25二羟基维生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA试剂盒厂家】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

QT-00417 铜绿假单胞杆菌（绿脓杆菌）     ATCC 27853      2支 美国 MBL        1400                  

QT-00418 铜绿假单胞杆菌（绿脓杆菌）     ATCC 27853      5支 美国 MBL        2620                  

QT-00419 铜绿假单胞杆菌（绿脓杆菌）     ATCC 9027        支     上海         460           

QT-00420 铜绿假单胞杆菌（绿脓杆菌）     ATCC 9027        2支 美国 MBL        1800                  

QT-00421 铜绿假单胞杆菌（绿脓杆菌）     ATCC 9027        5支 美国 MBL        3000                  

QT-00422 金黄色葡萄球菌     ATCC 27217      支     上海         800           

QT-00423 金黄色葡萄球菌     ATCC 6538        支     上海         460           

QT-00424 金黄色葡萄球菌     ATCC 6538        2支 美国 MBL        1700                  

QT-00425 金黄色葡萄球菌     ATCC 6538        5支 美国 MBL        3000                  

QT-00426 白色假丝酵母/白色念球菌   ATCC 10231      支     上海         400           

QT-00427 白色假丝酵母/白色念球菌   ATCC 10231      2支 美国 MBL        1810                  

QT-00428 白色假丝酵母/白色念球菌   ATCC 10231      5支 美国 MBL        3000                   【人1,25二羟基维生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA试剂盒厂家】

QT-00429 大肠埃希氏菌         ATCC 8739        支     上海         460           

QT-00430 大肠埃希氏菌         ATCC 8739        2支 美国 MBL        1550                  

QT-00431 表皮葡萄球菌         ATCC 12228      支     上海         400           

QT-00432 表皮葡萄球菌         ATCC 12228      2支 美国 MBL        1550                  

QT-00433 表皮葡萄球菌         ATCC 12228      5支 美国 MBL        2620                  

QT-00434 表皮葡萄球菌                   支     上海         460           

QT-00435 肺炎克雷伯菌                   支     上海         460           

QT-00436 阴沟肠杆菌              支     上海         500           

QT-00437 阴沟肠杆菌     ATCC 13047      支     上海         820           

QT-00438 洋葱假单胞菌         ATCC 25416      支     上海         820           

QT-00439 荧光假单胞菌                   支     上海         460           

QT-00440 荧光假单胞菌         ATCC 13525      支     上海         820           

QT-00441 恶臭假单胞菌                   支     上海         460           

QT-00442 恶臭假单胞菌         ATCC 17485      支     上海         820           

QT-00443 黄杆菌属         ATCC 21047      支     上海         820           

QT-00444 近平滑假丝酵母              支     上海         460            【人1,25二羟基维生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA试剂盒厂家】

QT-00445 黑曲霉     ATCC 16404      支     上海         460           

QT-00446 黑曲霉     ATCC 16404      2支 美国 MBL        1950                  

QT-00447 黑曲霉     ATCC 16404      5支 美国 MBL        3000                  

QT-00448 桔青霉              支     上海         460           

QT-00449 黄曲霉              支     上海         460           

9238662  Lid with window                CNY                             

9238659  Cooling compressor T2168GK, 60Hz                  CNY                             

9238645  Brake resistor 112 Ohm / 100 W               CNY                             

9242061  Pipetting container, IQ/OQ, QS                 CNY                             

9241827  Pyro Q96 ID, Premium Agreement "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9241166  Board, A/D, QX                  CNY                             

9240955  Sensor, z, handler, Qsym           CNY                             

9240835  QIAcube, Basic Agreement, no PM The Basic Agreement QIAcube provides full service coverage for the QIAcube. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Agreement, there is a priority response time of 5  working days.  CNY                             

9240766  32 OUT Module, D2-32TD1, AP96            CNY                             

6000-5018        MST STM Rack & Lid (Blue - 2 pos. cal.)            CNY                             

9241777  QIAxtractor, Inspection Service       "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAextractor are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY                              【人1,25二羟基维生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA试剂盒厂家】

9241375  Magnet, door lock, top, EZ1a           CNY                             

9241334  Keypad foil, EZ1                 CNY                             

9240750  Belt, Elevator, AP              CNY                             

9243648  Acceler. Valid Supp, Other Extract Sys     The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide provide an introduction to the instrument ａnd hands-on user support.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Attendees will receive a certificate of participation in the hands-on exercises.  Please indicate which protocols to validate (Biosprint 96, DNA mericon).          CNY                             

9243553  #BR M96, PM Service      "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9242864  QIArent&go -Rent out fee QSSP/AS Cabinet             CNY                             

5000-1321        Feet Set/4, SKR1                CNY                             

5000-1288        DML 2000 - Fuse 160 mA PKG 10              CNY                             

5000-1287        DML 2000 - Fuse 315 mA PKG 10              CNY                             

5000-1505        Switch ON-OFF 120V, HTR1               CNY                             

6000-00181      Plate Washer - Replacement Tubing Kit           CNY                             

6000-00178      Plate Washer - Maintenance Kit               CNY                             

5100-00047      Wash Buffer Concentrate (2 Bottles)       "2 bottles of 100mL each.

The price is proportional to 2x 5100-0795 (discontinued)"      CNY                             

9240740  Tubing, Tygon, 1/4" ID                CNY                             

9240735  Tubing, Black, Capper, AP196/98               CNY                             

9240724  Tube Assy, Draw, AP96/196                CNY                              【人1,25二羟基维生素D3(1,25-(OH)2D3/DVD/DHVD3)ELISA试剂盒厂家】

5000-1412        Poteneiometer 500K, VTX2               CNY                             

9240506  Monitor, 17", Flat Panel, AP             CNY                             

9239851  Cover, LC200             CNY                             

9239565  xMAP Alignment Microspheres                 CNY