【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【人非甲基化寡核苷酸(NON)ELISA试剂盒现货】样本保存条件：

1.2-8℃冰箱多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人非甲基化寡核苷酸(NON)ELISA试剂盒现货】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【人非甲基化寡核苷酸(NON)ELISA试剂盒现货】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人非甲基化寡核苷酸(NON)ELISA试剂盒现货】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

337820    qBiomarker SYBR ROX Mastermix (2)        CNY                           

9018917  Adapter, 72x0.1ml Strip Tubes             CNY                           

9018897  Rotor-Disc Pipetting Aid        CNY                           

9018708  EZ1 Adv. XL Virus Card v2.0        CNY                           

9018570  Guides, door, L/R, EZ1a        CNY                           

670213    ipsogen BCR-ABL1 mbcr (BCR) Kit (24)            CNY                           

389004    Investigatot IDproof Client Key            CNY                           

383615    Investigator Argus Y-12QS Kit (100)            CNY                           

383617    Investigator Argus Y-12QS Kit (400)            CNY                           

381315    Investigator Nonaplex ESS Kit (100)           CNY                           

130915    NeXtal DWBlock ProComplex Suite            CNY                           

130911    NeXtal DWBlock MBClass Suite          CNY                           

4801064  careHBV PCR ASSAY V2             CNY                           

6000-5025      MST Conversion System             CNY                           

L2133     Expand 4 Pipettor Manual (CD-ROM)         CNY                           

9240708  Filter, Vacuum, 40 Micron, AP196/98          CNY                            【人非甲基化寡核苷酸(NON)ELISA试剂盒现货】

9243656  Post-validation Competency Training    """Post-validation Competency Lab Training includes training of the laboratory DNA staff on QIAGEN instrumentation ａnd chemistry, ａnd a review of information of the validation design plan ａnd results of the validation study. Training will be presented both via seminar ａnd hands-on-learning. Attendees will receive a Certificate of Attendance from QIAGEN to provide documentation of training. As required by FBI regulations, the laboratory is then responsible for the competency testing of all DNA analysts to perform lab work validated. The training presentation ａnd curriculum vitae of the trainer may be provided upon request.

Note:

The Post-validation Competency Training is required for all Comprehensive Validation Study plans but can be included at zero cost.

The Post-validation Competency Training is optional for both Guided ａnd Accelerated Validation Study plans ａnd so charges should apply accordingly.

"""   CNY                           

9022641  therascreen KRAS Assay Package CE          CNY                           

5050-12200A  PC Country Kit, EU              CNY                           

274603    flocktype Mycoplasma Ms Ab (5)        CNY                           

285215    cador B. hyodysenteriae PCR Reagent (96)         CNY                           

273405    pigtype Toxoplasma Ab (20)         CNY                           

273801    pigtype Yersinia Ab (1)          CNY                           

280375    virotype BVDV RT-PCR Kit (96)          CNY                           

937156    QIAsymphony DSP AXpH DNA Kit (192)           CNY                           

9018702  EZ1 Adv. XL DSP DNA Blood Card             CNY                           

9001907  #QIAgility (w/o PC) PrioPLUS     "QIAgility (w/o PC)  Instrument

Installation of QIAgility instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"   CNY                           

800337    Transformation             CNY                           

9019616  ZLV Rail, worktable, plate, Cx2            CNY                           

9019882  #Lid with LED, blue, RG6            CNY                           

9020585  Spring - Gas, Door Support, DML 3000             CNY                           

9011930  Reagent holder, tubes ａnd bottles   Holder for accommodating 4 microtubes (1.5 ml), 4 tubes (13 ml), ａnd 2 bottles (125 ml ａnd 60 ml) on the worktable  CNY                            【人非甲基化寡核苷酸(NON)ELISA试剂盒现货】

9013456  ZLV Buchsenleiste Autocom 2 pol AWG28          CNY                           

       └ 脑微血管内皮细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 人脑成纤维细胞总DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 晶状体上皮细胞总DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 角膜上皮细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 视网膜色素上皮细胞总DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 视网膜微血管内皮细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 角膜成纤维细胞总DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 小梁网细胞总DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 视网膜muller细胞总DNA     1μg/1μl（10μg/20ug/50ug）                              

       └ 角膜内皮细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 脉络膜微血管细胞总DNA    1μg/1μl（10μg/20ug/50ug）                               【人非甲基化寡核苷酸(NON)ELISA试剂盒现货】

       └ 食道肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 胃肿瘤组织源性原代细胞总DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 肝肿瘤组织源性原代细胞总DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 结肠肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 直肠肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 肺肿瘤组织源性原代细胞总DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 胆道肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 肾肿瘤组织源性原代细胞总DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 膀胱肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 胰腺肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 甲状腺肿瘤组织源性原代细胞总DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 前列腺肿瘤组织源性原代细胞总DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 乳腺肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 卵巢肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 子宫肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 宫颈肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                               【人非甲基化寡核苷酸(NON)ELISA试剂盒现货】

       └ 皮肤组织肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 胶质肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 髓母细胞肿瘤组织源性原代细胞总DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 胃癌组织源性原代成纤维细胞总DNA 1μg/1μl（10μg/20ug/50ug）