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乳腺癌组织源性原代细胞
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发布日期:2016/7/22 16:39:18
更新日期:2016/7/22 16:39:18
详细内容
选择齐一生物的原因:
1.供货及时。我们订货周期短,运输时间短,在短时间内为您提供所需产品。
2.价格优惠:美国ATCC的中国代理商,没有中间环节。在国内我们保证价格。
3.服务保障:我们拥有专用的技术指导团队,对每售出的一件产品,提供全面的售前、售中和售后服务。
4.产品种类齐全:25个系统共150多种细胞以及各类菌株,满足不同客户的要求。细胞经过严格的质控,纯度可达98%。常用产品备有现货。其产品类型如下:消化系统细胞株,呼吸系统细胞株,泌尿系统细胞株生殖系统细胞株 血液循环系统 内皮细胞细胞株 神经系统及其他细胞株,动物细胞株和菌株
5.对于部分难培养的细胞我公司可代复苏细胞,并提供技术支持。
6.经验丰富:我公司是国内专业化的细胞销售公司,具有十余年细胞产品销售经验,有专业的销售团队和技术人员。齐一生物公司的每一位员工都将竭诚为您服务。
7.质量保证,保证售后
8. 齐一生物销售:021-6034 8496;181214 53965;173021 04490
【乳腺癌组织源性原代细胞】 齐一生物公司专业代理ScienCell简介ScienCell Research Laboratories (ScienCell) is a biotechnology company whose mission is the research and development of cell and cell-related products for experimental and therapeutic use. The ScienCell technology provides a variety of high quality normal human and animal cells, cell culture media and reagents, medium supplements, cell-derived RNA, cDNA and proteins for the research community. Scientists at ScienCell are also studying and developing cell therapeutic strategies that will significantly improve the quality of life by achieving the cell's ability to combat human degenerative disorders. We are based in San Diego, California and were established in 1999.
【乳腺癌组织源性原代细胞】美国ATCC简介:美国菌种保藏中心, 又称美国模式菌种收集中心(ATCC),是位于马里兰洲洛克菲勒的一家私营的,非赢利性组织。目前它可以提供各种动植物细胞、标准品、菌株达两万多种。ATCC成立于1925年,是世界上的生物资源中心,由美国14家生化、医学类行业协会组成的理事会负责管理,是一家全球性、非盈利生物标准品资源中心。ATCC向全球发布其获取、鉴定、保存及开发的生物标准品,推动科学研究的验证、应用及进步 ATCC可以提供以下类别生物标准品:细胞株(3000多种);菌株(15000多种);动植物病毒株(2500多种)以及重组物质等。
现已成为可信赖的活体微生物、细胞系等获得、保存和发放的国家资源中心。ATCC有29,000多种不同品系可靠的动物细胞和微生物培养体,它能满足各科学团体对可信赖品系的需要。仅1981年就有39,000多培养物发放给全世界的科学工作者,并成功地用冷冻和冷冻干燥的方法保存各种细胞品系。 ATCC是一个非(学会单位)无利润的公司,由各科学组织推选成员组成理事会进行管理。现有工作人员125人。参加的科学团体有:美国免疫协会、美国生物化学会、美国细胞生物学会、美国微生物学会、美国动物学会、美国遗传学会、原生动物学会和组织培养协会等16个学术组织。在ATCC可利用各种材料以及工具和设备进行广泛的研究。主要研究项目有:酶联免疫吸附分析技术(ELISA),真核细胞转录的调控,杂交瘤、淋巴类和髓类以及其他细胞系的建立,人突变细胞系的基因蛋白质,借助计算机进行鉴别分析,培养物的特征鉴别和保存技术的改进,关于化学致癌、致畸和致突变的。
【乳腺癌组织源性原代细胞】齐一生物提醒接收到细胞注意事项
1. 接收到细胞,肉眼观察细胞培养基颜色,显微镜观察细胞生长情况,并对细胞进行不同倍数拍照(建议收到时的培养瓶拍一张照片,显微镜拍收到时的细胞100X,200X各一张)。
2.用75%的酒精消毒(建议配置75%的酒精的水是灭菌过的)。
3.严格无菌操作,打开细胞培养瓶。
4.将细胞培养瓶内的培养基用吸管完全吸出(严禁直接倾倒),放入另一无菌容器中(建议换新的培养基培养细胞)。
5.用PBS 2-3ml/次轻轻洗细胞表面,洗3-4次遍,加入0.05%胰酶-EDTA1-1.5ml,显微镜下观察细胞变圆,轻轻拍打培养瓶直到细胞脱落,加入终止液终止。
6.1000rpm,5min离心,重悬细胞。
7.换新的细胞培养瓶,37℃,5%CO2培养。【乳腺癌组织源性原代细胞】
齐一生物销售:021-6034 8496;181214 53965;173021 04490
204752 QuantiFast Multiplex PCR +R Kit (80) CNY
290095 mericon Quant. L. pneumophila Kit (96) CNY
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9015353 Controller, temp. CNY
133051 NeXtalStock HEPES sod. salt untit. (200) CNY
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951054 EZ1 DNA Blood 350 µl Kit (48) "
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9001874 EZ1 Advanced XL, System EZ1 Advanced XL, System combines the EZ1 Advanced XL instrument and installation. The Installation and Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation and user maintenance. All travel and labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY 【乳腺癌组织源性原代细胞】
9015466 Cover, P CNY
9012413 Z-Bars Gripper, RCS1 CNY
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205110 Omniscript RT Kit (10) For 10 reverse-transcription reactions: 40 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water CNY
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133178 NeXtalStock Citric acid pH 2.6 (200) CNY
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9018409 Regulator, N2, internal, front, QX CNY
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9001861 Rotor-Gene Q 2plex (PC) Priority Rotor-Gene Q 2plex (PC) Priority includes the Rotor-Gene Q system, installation and extended warranty. Installation covers hardware and software installation of system and provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor and travel costs with installation performance and service visits documented on a GMP/GLP-compliant Field Service documents. CNY
9012327 Peristaltic Pump Outlet Tubing CNY
1067925 Buffer AW1 (175 ml) CNY
38045 GNA Cartridge (5ml) CNY
38003 ConA Cartridges (3 x 1ml) CNY
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204741 QuantiTect Multiplex PCR NoROX Kit (40) "Trial kit size for 40 x 50 ul reactions: 1.7 ml QuantiTect Multiplex PCR NoROX Master Mix, 2.0 ml RNase-free water
For quantitative, real-time PCR and two-step RT-PCR using probe detection with multiple assays." CNY 【乳腺癌组织源性原代细胞】
133066 NeXtalStock Manganese(II) chloride (200) CNY
290065 mericon Cronobacter spp Kit (96) CNY
204374 Rotor-Gene Probe PCR Kit (400) The Rotor-Gene Probe PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly sensitive quantification in real-time PCR and two-step RT-PCR using sequence-specific probes. CNY
QYD554 菌落总数定量计数板 96孔/盒
QYD555 大肠菌群定量计数板 96孔/盒
QYD556 金黄色葡萄球菌定量计数板 96孔/盒
QYD557 大肠杆菌定量计数板 96孔/盒
QYD558 大肠杆菌/大肠菌群定量计数板 96孔/盒
QYD559 酵母及霉菌定量计数板 96孔/盒
QYD560 肠杆菌定量计数板 96孔/盒 【乳腺癌组织源性原代细胞】
QYD561 沙门氏菌/肠杆菌定量计数板 96孔/盒
QYD562 大肠杆菌O157快速检测试剂盒 96孔/盒
QYD563 沙门氏菌快速检测试剂盒 96孔/盒
QYD564 李斯特菌快速检测试剂盒 96孔/盒
QYD565 沙门氏菌免疫胶体金试剂盒 96孔/盒
QYD566 大肠杆菌O157免疫胶体金试剂盒 96孔/盒
QYD567 单增李斯特菌免疫胶体金试剂盒 96孔/盒
QYD568 李斯特菌免疫胶体金试剂盒 96孔/盒
QYD569 弯曲杆菌免疫胶体金试剂盒 96孔/盒
QYD570 大肠杆菌肠毒素(VT1、VT2)免疫胶体金试剂盒 96孔/盒
QYD571 蜡样芽胞杆菌毒素免疫胶体金试剂盒 96孔/盒
QYD572 军团菌免疫胶体金试剂盒 96孔/盒
QYD573 新城疫ELISA试剂盒(NDV-Ab) 96孔/盒
QYD574 传染性法氏囊ELISA试剂盒(IBD-Ab) 96孔/盒
QYD575 传染性支气管炎ELISA试剂盒 (IBV-Ab) 96孔/盒
QYD576 病毒性关节炎ELISA试剂盒 (REO-Ab) 96孔/盒 【乳腺癌组织源性原代细胞】
QYD577 禽鼻气管炎ELISA试剂盒 (ART-Ab) 96孔/盒
QYD578 减蛋综合症ELISA试剂盒 (EDS-Ab) 96孔/盒
QYD579 败血支原体ELISA试剂盒 (MG-Ab) 96孔/盒
QYD580 滑液支原体快速诊断试剂盒(MS-Ab) 96孔/盒
QYD581 火鸡支原体ELISA试剂盒 (MM-Ab) 96孔/盒
QYD582 肠炎沙门氏菌ELISA试剂盒(SE-Ab) 96孔/盒
QYD583 鼠伤寒沙门氏菌ELISA试剂盒 (ST-Ab) 96孔/盒
QYD584 鼻气管炎鸟杆菌ELISA试剂盒 (ORT-Ab) 96孔/盒
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QYD553 李斯特菌检测试剂盒 96孔/盒
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