【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【鸡流行性乙型脑炎抗体IgG(JEIgG)ELISA试剂盒现货】样本保存条件：

1.2-8℃冰箱多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【鸡流行性乙型脑炎抗体IgG(JEIgG)ELISA试剂盒现货】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【鸡流行性乙型脑炎抗体IgG(JEIgG)ELISA试剂盒现货】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【鸡流行性乙型脑炎抗体IgG(JEIgG)ELISA试剂盒现货】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

132546    NeXtal Stock Ferric chloride (50) 1M Ferric Chloride Hexahydrate - 50mL (4 x 12,5 ml tube) CNY                           

132008    EasyXtal 15-Well Tool X-Seal (20)       "20 empty 15-well crystallization plates with X-Seal crystallization supports

For manual setup of hanging ｄrop crystallization experiments."   CNY                           

203645    HotStarTaq Plus Master Mix Kit (1000)       "For 1000 x 20 µl reactions: 12 x 0.85 ml HotStarTaq Plus Master Mix, containing 1000 units of HotStarTaq Plus DNA Polymerase total, 4 x 0.55 ml CoralLoad Concentrate, 8 x 1.9 ml RNase-Free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."     CNY                           

203603    HotStarTaq Plus DNA Polymerase (250)      1 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2 CNY                           

203601    HotStarTaq Plus DNA Polymerase (50) Non published.       CNY                           

201912    dNTP Set, PCR Grade 100mM, (4x100µl)   dNTP Set is a complete set of individual, highly pure dNTPs: 100 mM each dATP, dCTP, dGTP, dTTP for 1000 x 50ul PCR reactions   CNY                           

201223    Taq PCR Core Kit (250) "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."  CNY                           

180840    GeneRead qPCR SYBR Green/ROX MM (2)              CNY                           

180830    GeneRead qPCR SYBR Green/Fluor MM (2)              CNY                           

158767    Gentra Puregene Cell Kit (8 x 108)              CNY                           

34650     RGS·His Antibody, BSA-free (100 µg)  100 ug mouse anti-RGS(H)4 (lyophilized, BSA-free, for 1000 ml working solution)       CNY                           

32582     EasyXpress Disulfide Insect 10 rxns Kit             CNY                            【鸡流行性乙型脑炎抗体IgG(JEIgG)ELISA试剂盒现货】

32149     QIAexpress Type IV Kit 5 ug each: pQE-30, pQE-31, pQE-32 (N-terminal 6xHis); 10 ml Ni-NTA Agarose. pQE-40       CNY                           

31014     Ni-NTA Spin Columns (50)   50 Ni-NTA Spin Columns, Collection Tubes CNY                           

30600     Ni-NTA Fast Start Kit (6)             CNY                           

9021350  Piston, connecting rod, TLyser LT              CNY                           

9022027  Sensor, Y-axis, with cable, QXA           CNY                           

9002142  careHPV test Controller         CNY                           

286115    cador N. caninum PCR Reagent (96)           CNY                           

282603    virotype Influenza A RT-PCR Kit (24)          CNY                           

282607    virotype Influenza A RT-PCR Kit (480)        CNY                           

280373    virotype BVDV RT-PCR Kit (24)          CNY                           

280445    virotype BTV pan/8 RT-PCR Kit (96)           CNY                           

9018703  EZ1 Adv. XL DSP Virus Card        CNY                           

9001252  BIOROBOT 8000 Platf.Promo,Reaction Setup            CNY                           

9019515  ZLV Y-piece, pinch valve tubing, CX1          CNY                           

9020575  LED Assy, Green, 15" Long, DML 3000             CNY                           

9020593  Lid, DML3000              CNY                            【鸡流行性乙型脑炎抗体IgG(JEIgG)ELISA试剂盒现货】

9022383  Original Transport Box, QXA              CNY                           

9013308  Base, vacuum manifold, v1.04             CNY                           

9013298  Top plate, vacuum manifold96 plate , 4        CNY                           

QY-x1894       SGC7901 胃低分化腺癌 SK-OV-3 卵巢腺癌                                     

QY-x1895       HuTu-80 十二指肠腺癌 SKOV-3/DDP 卵巢癌顺铂耐药株                                      

QY-x1896       Caco-2 结肠癌 HeLa 宫颈癌                                       

QY-x1897       COLO 205 结肠癌 HelaS3 宫颈癌                                      

QY-x1898       COLO 320DM 结肠癌 HEC-1B 子宫内膜癌                                      

QY-x1899       HCT-8 回盲肠腺癌 JEG-3 绒癌                                         

QY-x1900       HCT-116 低分化结肠腺癌 Du-145前列腺癌                                     

QY-x1901       HT-29 结肠腺癌 LNCaP 前列腺癌                                     

QY-x1902       LoVo 结肠癌 LNCaP clone FGC 前列腺癌                                        

QY-x1903       SW480 结肠癌                                       

QY-x1904       PC-3 前列腺癌                                     

QY-x1905       BEL-7402 肝细胞癌 Tsu-Prl非雄激素依赖型前列腺癌                                    

QY-x1906       HCC-9204 肝癌HOS 骨肉瘤                                        【鸡流行性乙型脑炎抗体IgG(JEIgG)ELISA试剂盒现货】

QY-x1907       Hep3B 肝癌 MG-63 成骨肉瘤                                     

QY-x1908       Hep G2 肝癌 Saos-2                                      

QY-x1909       骨肉瘤                                          

QY-x1910       SMMC-7721 肝癌 U-2 OS 骨肉瘤                                     

QY-x1911       PANC-1胰腺导管上皮癌OS-732 骨肉瘤（瘤株）                                          

QY-x1912       Panc 10.05 胰腺腺癌 A-204 横纹肌肉瘤                                          

QY-x1913       SW1990胰腺腺癌HT1080纤维肉瘤                                    

QY-x1914       SK-RC-42 肾癌 A375 皮肤黑色素瘤                                         

QY-x1915       RCC-krause 肾癌 A875 黑色素瘤                                      

QY-x1916       Ketr-3 肾癌（瘤株） SK-HEL-1 皮肤黑色素瘤                                       

QY-x1917       SW 13 肾上腺皮质瘤 A431 皮肤基底细胞癌                                          

QY-x1918       95-C 低转移肺癌 CEM 白血病                                          

QY-x1919       95-D 高转移肺癌 K562 慢性髓原白血病                                   

QY-x1920       973 肺腺癌 HPB-ALLT细胞白血病                                    

QY-x1921       A2 肺腺癌 HL-60 白血病                                    

QY-x1922       A549 肺腺癌 Daudi B淋巴细胞瘤                                       【鸡流行性乙型脑炎抗体IgG(JEIgG)ELISA试剂盒现货】

QY-x1923       A549/DDP 肺腺癌/顺铂耐药株 Raji Burkitt淋巴瘤