1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！【鸡血小板因子4(PF-4/CXCL4)ELISA试剂盒现货】

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【鸡血小板因子4(PF-4/CXCL4)ELISA试剂盒现货】齐一生物销售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【鸡血小板因子4(PF-4/CXCL4)ELISA试剂盒现货】ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性.

以上资料仅供参考了解，不供于论文写作

【鸡血小板因子4(PF-4/CXCL4)ELISA试剂盒现货】齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

9238645 Brake resistor 112 Ohm / 100 W      CNY            

9242061 Pipetting container, IQ/OQ, QS      CNY            

9241827 Pyro Q96 ID, Premium Agreement  "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9241166 Board, A/D, QX      CNY            

9240955 Sensor, z, handler, Qsym        CNY            

9240835 QIAcube, Basic Agreement, no PM The Basic Agreement QIAcube provides full service coverage for the QIAcube. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Agreement, there is a priority response time of 5  working days.   CNY            

9240766 32 OUT Module, D2-32TD1, AP96       CNY            

6000-5018   MST STM Rack & Lid (Blue - 2 pos. cal.)     CNY            

9241777 QIAxtractor, Inspection Service "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAextractor are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY            

9241375 Magnet, door lock, top, EZ1a        CNY            

9241334 Keypad foil, EZ1        CNY            

9240750 Belt, Elevator, AP      CNY            

9243648 Acceler. Valid Supp, Other Extract Sys  The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide provide an introduction to the instrument ａnd hands-on user support.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Attendees will receive a certificate of participation in the hands-on exercises.  Please indicate which protocols to validate (Biosprint 96, DNA mericon).    CNY            

9243553 #BR M96, PM Service "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY             【鸡血小板因子4(PF-4/CXCL4)ELISA试剂盒现货】

9242864 QIArent&go -Rent out fee QSSP/AS Cabinet        CNY            

5000-1321   Feet Set/4, SKR1        CNY            

5000-1288   DML 2000 - Fuse 160 mA PKG 10       CNY            

5000-1287   DML 2000 - Fuse 315 mA PKG 10       CNY            

5000-1505   Switch ON-OFF 120V, HTR1        CNY            

6000-00181  Plate Washer - Replacement Tubing Kit       CNY            

6000-00178  Plate Washer - Maintenance Kit      CNY            

5100-00047  Wash Buffer Concentrate (2 Bottles) "2 bottles of 100mL each.

The price is proportional to 2x 5100-0795 (discontinued)"   CNY            

9240740 Tubing, Tygon, 1/4" ID      CNY            

9240735 Tubing, Black, Capper, AP196/98     CNY            

9240724 Tube Assy, Draw, AP96/196       CNY            

5000-1412   Poteneiometer 500K, VTX2        CNY            

9240506 Monitor, 17", Flat Panel, AP        CNY            

9239851 Cover, LC200        CNY            

9239565 xMAP Alignment Microspheres     CNY            

9019942 Barrel removal tool, CX1        CNY             【鸡血小板因子4(PF-4/CXCL4)ELISA试剂盒现货】

9019474 Lid, UV, polycarb, C1x      CNY            

QY-x1954    COLO 205 结肠癌 1640+10%胎牛血清                        【鸡血小板因子4(PF-4/CXCL4)ELISA试剂盒现货】

QY-x1955    COLO320DM 结肠癌 1640+10%胎牛血清                      

QY-x1956    HCT-8 回盲肠腺癌 1640+10%胎牛血清                      

QY-x1957    HCT-116 低分化结肠腺癌 1640+10%胎牛血清                    

QY-x1958    HT-29 结肠腺癌 1640+10%胎牛血清                    

QY-x1959    LS-174T 结肠腺癌 MEM+非必需氨基酸+10%胎牛血清                      

QY-x1960    SW480 结肠癌 L15                       

QY-x1961    BEL-7402 肝细胞癌 1640+10%胎牛血清                     

QY-x1962    BEL-7405 肝细胞癌 1640+10%胎牛血清                     

QY-x1963    H7402 肝癌 1640+10%胎牛血清                    

QY-x1964    HCC-9204 肝癌 1640+10%胎牛血清                     

QY-x1965    Hep3B 肝癌 DMEM+非必需氨基酸+10%胎牛血清                       

QY-x1966    Hep G2 肝癌 DMEM+10%胎牛血清                       

QY-x1967    SMMC-7721 肝癌 1640+10%胎牛血清                    

QY-x1968    PANC-1 胰腺导管上皮癌 1640+10%胎牛血清                     

QY-x1969    Panc 10.05 胰腺腺癌 高糖1640+15%胎牛血清+胰岛素等                      

QY-x1970    SW1990 胰腺腺癌 1640+10%胎牛血清                        【鸡血小板因子4(PF-4/CXCL4)ELISA试剂盒现货】

QY-x1971    JF305 胰腺癌 1640+10%胎牛血清                      

QY-x1972    PC-3 胰腺癌 1640+10%胎牛血清                       

QY-x1973    Capan-2 胰腺癌 1640+10%胎牛血清                    

QY-x1974    SK-RC-42 肾癌 1640+10%胎牛血清                     

QY-x1975    95-C 低转移肺癌 1640+10%胎牛血清                       

QY-x1976    95-D 高转移肺癌 1640+10%胎牛血清                       

QY-x1977    973 肺腺癌 1640+10%胎牛血清                    

QY-x1978    A2 肺腺癌 1640+10%胎牛血清                     

QY-x1979    A549 肺腺癌 1640+10%胎牛血清                       

QY-x1980    A549/DDP 肺腺癌/顺铂耐药株 1640+10%胎牛血清                    

QY-x1981    Calu-3 肺腺癌 MEM+非必需氨基酸+10%胎牛血清                     

QY-x1982    GLC-82 肺腺癌 1640+10%胎牛血清 已有                    

QY-x1983    H1299 非小细胞肺腺癌 1640+10%胎牛血清