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鸡可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA试剂盒现货

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发布日期:2016/8/1 21:20:13

更新日期:2016/8/2 16:42:16

详细内容

 鸡可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA试剂盒现货齐一生物供应的ELISA试剂盒试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器,EP 管及一次性吸头

3. 37℃恒温箱,双蒸水或去离子水

4. 吸水纸

鸡可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA试剂盒现货齐一生物【友情提示】本产品仅供科研研究使用,不得用于人体临床直接检测。避免给您带来不必要的损失,请仔细阅读购买说明!

ELISA应用:

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎(HEV)病毒IgM抗体ELISA检测

鸡可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA试剂盒现货齐一生物科技欢迎您订购咨询,齐一生物销售:0216034 8496181214 53965173021 04490

齐一生物供应的ELISA试剂盒安全性提示

1.工作人员实验中避免直接接触终止液和底物AB。一旦接触到这些液体,请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件:

1.2-8℃冰箱多放2

2.-20-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱,可以放一年

鸡可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA试剂盒现货齐一生物供应的ELISA试剂盒ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验(ELISA.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物AB,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点(检测模式:临床抗体夹心法):包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性(99.98%)提高检测的灵敏度,应用ParlEhrich学说(PEIHBsAg标准品,ad标准品的灵敏度为0.05ng/mlay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP(辣根过氧化物酶)酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性. 鸡可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA试剂盒现货

以上资料仅供参考了解,不供于论文写作

齐一生物专业为您提供高品质,高灵敏度.高质量的酶联免疫试剂盒,齐一生物长期经营ELISA试剂盒(免费代测)及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售:0216034 8496181214 53965173021 04490

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9240987  Kit, electronic, FFC-plugs, Qsym                CNY                             

9240980  Sensor, tray, sample tracking, Qsym                  CNY                             

9240945  Sensor, xy, portal, Qsym            CNY                             

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9240783  Update, Weigh Cell, AP96                  CNY                             

9240774  Assy, Transfer Rack, AP96                  CNY                             

9241632  LED Light System, AP                 CNY                              【鸡可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA试剂盒现货】

9241369  Temperature Controller, EZ1a                   CNY                             

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9243542  BR MDx, PM Service        "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9243305  Power Cord, India             CNY                             

9243110  CD-RW, Black, AP196/98           CNY                             

9242712  Validation Support, 2d QIAcube       The QIAGEN QIAcube Guided Validation includes all travel and labor charges for a site visit up to 2 days by a QIAGEN Specialist. A QIAGEN specialist will provide an introduction to the QIAcube system and the DNA Investigator silica chemistry utilized in the process, review the Handbook and User Manual, as well as hands-on QIAcube operation.  The Guided Validation service includes experimental design consulation, internal validation documentation support, and QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry and accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design and are not included in the cost of validation support.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.)      CNY                             

9019226  Fan - rear 92x92, PyroQ24                 CNY                             

9019220  PCB - Distribution robot, PyroQ96MD               CNY                             

1066843  PG Influenza A RT-PCR Assay (48) LE Z              CNY                             

1066848  NDV Univ. RT-PCR REAGENT LE                 CNY                             

1066854  AIV-H9 RT-PCR REAGENT LE             CNY                             

1060992  Buffer MW1 (77ml)           CNY                             

1061059  Sample Preparation Reagents, 8ml                   CNY                             

1061080  NG Quantification Standards            CNY                             

1061113  PG AIV-H7 RT-PCR Assay (48) Z                  CNY                             

9016568  Plate Grippers, RCS1                  CNY                              【鸡可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA试剂盒现货】

9016536  Transportation box , BioSprint 15             CNY                             

9016535  Transportation box , Twister II                   CNY                             

9016533  Transportation box , RapidPlate onlf.                CNY                             

9016530  Transportation box 1, LiquiChip                 CNY                             

QY-x2344 Ca Ski 人宫颈癌肠转移细胞RPMI 1640培养基,添加2 mM L-谷氨酰胺,含1.5 g/L 碳酸氢钠, 4.5 g/L 葡萄糖, 10 m...                                                        

QY-x2345 C6大鼠胶质瘤细胞 含有2mM L-谷氨酰胺的F12K,加1.5/升碳酸氢钠,添加15%马血清,2.5%胎牛血清。 胶质细胞株C6...                                                 

QY-x2346 C2C12小鼠成肌细胞mouse组织                                               

QY-x2347 BxPC-3 人原位胰腺腺癌细胞 1.5g/L 碳酸氢钠, 4.5g/L 葡萄糖, 10mM HEPES1.0mM 丙酮酸钠和2mM L-谷氨酰胺的RPM...                                                 

QY-x2348 BT-549 人乳腺管癌细胞 1.5g/L 碳酸氢钠, 4.5g/L 葡萄糖, 10mM HEPES, 2mM L-谷氨酰胺和0.023单位/ml胰岛素...                                                  

QY-x2349 BHK-21 仓鼠幼仓鼠肾细胞RPMI 1640DMEM + 20% 胎牛血清或新生牛血清. -                                                     

QY-x2350 BGC-823人胃腺癌细胞(低分化)/62RPMI 1640 + 10% 胎牛血清或新生牛血清. 建自一位62岁的胃癌患者。                                                    

QY-x2351 BEL-7402 人肝癌细胞 RPMI 1640 + 10% 胎牛血清或新生牛血清。 BEL-7402细胞株是1974年从临床肝癌手术标本中建立的。 ...                                                    

QY-x2352 B16 小鼠黑色素瘤细胞RPMI 1640 + 10% 胎牛血清或新生牛血清. 小鼠黑素瘤细胞。                                                     

QY-x2353 AsPC-1 人转移胰腺腺癌细胞 1.5g/L 碳酸氢钠, 4.5g/L 葡萄糖, 10mM HEPES1.0mM 丙酮酸钠和2mM L-谷氨酰胺的...                                                   

QY-x2354 Ana-1小鼠巨噬细胞RPMI 1640 + 10% 胎牛血清或新生牛血清。 -                                                        【鸡可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA试剂盒现货】

QY-x2355 AAV-293人胚肾细胞含4.5g/L葡萄糖,110mg/L丙酮酸钠,4mM L-谷氨酰胺的DMEM90%;热灭活胎牛血清 1...                                                        

QY-x2356 A549 [A-549]人肺癌细胞 2mM L-谷氨酰胺的F12K,加1.5/升碳酸氢钠,添加10%胎牛血清。 该细胞系由D.J.Griad通过肺癌...                                                  

QY-x2357 A-431人表皮癌细胞 1.5g/L 碳酸氢钠, 4.5g/L 葡萄糖,4mM L-谷氨酰胺的DMEM90%;胎牛血清或新生牛血清,10%...                                                

QY-x2358 A-375[A375] 人恶性黑色素瘤细胞含1.5g/L 碳酸氢钠, 4.5g/L 葡萄糖,4mM L-谷氨酰胺的DMEM90%;胎牛血清或新...                                                      

QY-x2359 A3T淋巴细胞白血病细胞,T淋巴细胞形态: 淋巴母细胞含2mM L-谷氨酰胺,10mM HEPES1mM丙酮酸钠,4.5g/L葡萄糖和1.5g/L 碳酸氢钠的R...                                                  

QY-x2360 95-D人高转移肺癌细胞RPMI 164090%;胎牛血清或新生牛血清 10% 这是一株高转移肺癌。                                                       

QY-x2361 786-O人肾透明细胞腺癌细胞 1.5g/L 碳酸氢钠, 4.5g/L 葡萄糖, 10mM HEPES1.0mM丙酮酸钠,和2mM L-谷氨酰胺...                                                   

QY-x2362 6T-CEM T细胞白血病细胞急性淋巴细胞白血病形态: 淋巴母细胞RPMI 1640 + 10% 胎牛血清或新生牛血清。 这是一个CCRF-CEM6-... 小鼠脂肪细胞 3T3-L1 询价 ATCC                                                

QY-x2363 3T3-L1 小鼠脂肪细胞含1.5g/L 碳酸氢钠, 4.5g/L 葡萄糖,4mM L-谷氨酰胺的DMEM90%BCS10% L1是通过... 人胚肾细胞 293T 询价 ATCC                                                  

QY-x2364 293T人胚肾细胞293T1.5g/L 碳酸氢钠, 4.5g/L 葡萄糖, 4mM L-谷氨酰胺的DMEM90%;胎牛血清,10% 293细胞株插入...                                                

QY-x2365 22RV1人前列腺癌细胞 10mM HEPES, 1mM 丙酮酸钠,4.5g/l 葡萄糖和1.5g/l碳酸氢钠和2mM L-谷氨酰胺的RPMI 1640;                                                    

QY-x2366 5637人膀胱癌细胞 膀胱;癌形态: /年龄: 1.5g/L 碳酸氢钠, 4.5g/L 葡萄糖, 10mM HEPES1.0mM丙酮酸钠,和2mM L-谷氨酰胺的RPMI 16...                                                  

QY-x2367 293 人胚肾细胞肾;以腺病毒5 DNA 进行转化。 1.5g/L 碳酸氢钠,0.1mM 非必需氨基酸,1.0mM 丙酮酸钠,2mM L-谷氨酰胺和Earle&...                                                       

QY-x2368 大鼠肝细胞BRL                                                   

QY-x2369 人肝母细胞瘤细胞HUH-6                                                   

QY-x2370 小鼠淋巴瘤细胞YAC-1                                                 【鸡可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA试剂盒现货】

QY-x2371 人羊膜细胞WISH                                                

QY-x2372 人胚肺细胞WI-38                                               

QY-x2373 非洲绿猴肾细胞Vero                                                   

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