价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供优质产品。

【鸡白介素5(IL-5)ELISA试剂盒厂家】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【鸡白介素5(IL-5)ELISA试剂盒厂家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【鸡白介素5(IL-5)ELISA试剂盒厂家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【鸡白介素5(IL-5)ELISA试剂盒厂家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

5123-1220        Female Swab Specimen Collection KitTM                  CNY                             

5100-1400IVD hc2 Sample Conversion Kit                CNY                             

EA11424  EASYartus BKV RG_II                 CNY                             

5050-1075 V.1 CD Rom Manual, MST Vortexer 2             CNY                             

EA10124  EASYartus EBV RG PCR Kit 2             CNY                             

BR-02       DxS B-RAF Mutation Test Kit            CNY                             

9240729  Tubing, 3/8"OD , Red, AP          CNY                             

9240719  Cable Carrier, Capper, Elevator, AP          CNY                             

9240701  O-Ring, Shaker, Lower Body, AP96/196             CNY                             

9240696  Fitting, Barbed, 1/4" ID tube, AP               CNY                             

9243651  Comprehen Valid., QIAgility     """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

218076     miScript SYBR Green PCR Kit (2000)                  CNY                             

271906     cattletype Milk Prep Kit (50)             CNY                             

289980     intype IC-DNA (1ml)                   CNY                              【鸡白介素5(IL-5)ELISA试剂盒厂家】

280437     virotype BTV RT-PCR Kit (480)          CNY                             

932836     #QIAsymphony Ni-NTA Denat. Kit (384)           CNY                             

800511     Verification Sequencing / DNA production                CNY                             

9238243  ZLV BioRobot M96 User Manual               CNY                             

9019988  ZLV Mainboard, QGY                 CNY                             

9020553  Foam angle, transport box, QIAC              CNY                             

9020582  X-Motor, DML3000           CNY                             

9020584  Y-Belt, DML3000 320 Teeth              CNY                             

9020594  Enclosure, DML3000                 CNY                             

9021006  Motor, Pipettor Y, DCU             CNY                             

9236333  Sample Loop              CNY                             

9013382  Shaker adapter, microplate, LHS              CNY                             

9013275  Motor, gripper, handler T/L, BR3000                CNY                             

9013269  Fitting, PA tubing M6x1, 2.25mm, (10)            CNY                             

9013210  Cabling kit, moving, BR8000             CNY                             

9013198  Tubing,pres.sen.con.auto vac,BR8000v1.00            CNY                              【鸡白介素5(IL-5)ELISA试剂盒厂家】

9013188  Valve set, pressure relaese, dispenser,             CNY                             

QY-x2434 人肝癌细胞 Hep 3B2.1-7 询价 ATCC                                                         【鸡白介素5(IL-5)ELISA试剂盒厂家】

QY-x2435 人宫颈癌细胞 Hela 询价 ATCC                                               

QY-x2436 大鼠心肌细胞 H9c2(2-1) 询价 ATCC                                                        

QY-x2437 人乳腺细胞 HBL-100 询价 ATCC                                                      

QY-x2438 人T淋巴细胞系 H9 询价 ATCC                                                        

QY-x2439 小鼠淋巴瘤细胞 EL-4 询价 ATCC                                                     

QY-x2440 人前列腺癌细胞 DU 145 询价 ATCC                                                        

QY-x2441 人成巨核细胞白血病细胞 Daudi 询价 ATCC                                                  

QY-x2442 非洲绿猴SV40转化的肾细胞 COS-7 询价 ATCC                                                    

QY-x2443 人结肠腺癌细胞 COLO 320 询价 ATCC                                                    

QY-x2444 人鼻咽癌细胞 CNE 询价 ATCC                                                

QY-x2445 人肺腺癌 (胸水) Calu-3 询价 ATCC                                                  

QY-x2446 人急性淋巴细胞白血病T淋巴细胞 CCRF-CEM 询价 ATCC                                                   

QY-x2447 人肾透明细胞癌皮肤转移细胞 Caki-1 询价 ATCC                                                        

QY-x2448 Burkitts淋巴癌细胞系 CA46 询价 ATCC                                                

QY-x2449 人宫颈癌肠转移细胞 Ca Ski 询价 ATCC                                                  

QY-x2450 大鼠胶质瘤细胞 C6 询价 ATCC                                                         【鸡白介素5(IL-5)ELISA试剂盒厂家】

QY-x2451 人乳腺管癌细胞 BT-549 询价 ATCC                                               

QY-x2452 仓鼠幼仓鼠肾细胞 BHK-21 [C-13] 询价 ATCC                                                 

QY-x2453 人肝癌细胞 BEL-7402 询价 ATCC                                                  

QY-x2454 小鼠黑色素瘤细胞 B16 询价 ATCC                                                 

QY-x2455 人转移胰腺腺癌细胞 AsPC-1 询价 ATCC                                                

QY-x2456 人胚肾细胞 AAV-293 询价 ATCC                                                      

QY-x2457 人肺癌细胞 A549 [A-549] 询价 ATCC                                                       

QY-x2458 人表皮癌细胞 A-431 询价 ATCC                                                      

QY-x2459 人T淋巴细胞白血病细胞 A3 询价 ATCC                                                        

QY-x2460 人高转移肺癌细胞 95-D 询价 ATCC                                                        

QY-x2461 人肾透明细胞腺癌细胞 786-O 询价 ATCC                                                      

QY-x2462 小鼠脂肪细胞 3T3-L1 询价 ATCC                                                     

QY-x2463 人胚肾细胞 293T 询价 ATCC