1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！【大鼠二肽基肽酶Ⅳ(DPPⅣ)ELISA试剂盒现货】

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【大鼠二肽基肽酶Ⅳ(DPPⅣ)ELISA试剂盒现货】齐一生物销售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【大鼠二肽基肽酶Ⅳ(DPPⅣ)ELISA试剂盒现货】ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性.

以上资料仅供参考了解，不供于论文写作

【大鼠二肽基肽酶Ⅳ(DPPⅣ)ELISA试剂盒现货】齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

9230405 Pipetting Position Ground Plate     CNY             【大鼠二肽基肽酶Ⅳ(DPPⅣ)ELISA试剂盒现货】

9230189 Safety shield for size 0        CNY            

9022808 LED Barrel, QXA     CNY            

9238656 Cable, 50-channel       CNY            

9238647 Mechanical lock with optic, left        CNY            

9238631 Keypad, foil        CNY            

9242083 Insert, 2.0ml v2, samplecarr. (24), Qsym        CNY            

9242071 External BCR, Metrologic, MS3780, AP        CNY            

9242062 Cover, evaporation trap, IQ/OQ, QS      CNY            

9241893 QIArent&go - Rent out fee QIAxtractor       CNY            

9241826 Pyro Q24, Premium Agreement "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9241817 Pyro Q96 MDa, PM Agreement      CNY            

9241174 Power supply, main, 24V, QX     CNY            

9241008 QIArent&go - Rent out fee QIAsymphony       CNY            

9240958 Gripper, handler, Qsym      CNY            

9240818 Performance, certification, EZ1     CNY            

9234855 Lid coupling piece for washer liquid        CNY             【大鼠二肽基肽酶Ⅳ(DPPⅣ)ELISA试剂盒现货】

9240784 Update, Weigh Cell, AP98        CNY            

9240773 Assy, Shaker, AP96      CNY            

9241775 QIAxtractor, Installation   The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the QIAxtractor systems hardware ａnd trains the user on the operation of the software. After the training the user will be able to run ａnd install application programs ａnd conduct a basic troubleshooting. All travel ａnd labor costs are included. The service is documented in accordance with GMP/GLP requirements.   CNY            

9241631 Capper Mounting Kit, AP     CNY            

9243209 APAC-HC2 Service Package 5yrs       CNY            

9243043 Panel, transparent, Qsym        CNY            

9242767 Cogwheel, y-drive, portal, Qsym     CNY            

9242762 QS RGQ, Basic Agreement "Repair service delivery QIAsymphony SP/AS: onsite

Service response time (repair) QIAsymphony: 5 business days

Repair service delivery RGQ: send to Regional Repair Center

Service response time (repair) RGQ: 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision RGQ: not applicable

Transportation cost coverage: yes, full coverage"   CNY            

9242716 Validation Support, 5d, QIAsymphony The QIAGEN QIAsymphony Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Applications Specialist.  A QIAGEN specialist will provide an introduction to the QIAsymphony system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user support ａnd QIAsymphony instrument operation. The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product. Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to include in the validation (Casework200, Casework500, Reference200, etc.).    CNY            

9242548 Cheetah Upgrade Kit, AP98       CNY            

9242532 QIAsymphony AS, Installation    The Installation, QIAsymphony AS facilitates the quick implementation of the QIAsymphony into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the QIAsymphony instrument ａnd provides the user with an introductory training. The installation includes or labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.  CNY            

9242155 Loaner system (1 time rental)       CNY            

5000-1322   Cord Line 115V, SKR1        CNY            

5000-1318   Base Plate/Upper Body, SKR1     CNY            

5000-1310   Gasket Rubber, SKR1     CNY            

QY-06890    PEPT1 (Peptide-transporters 1)肠道肽转运蛋白(小肽转运蛋白)多肽       0.5mg/6501mg/1100             

QY-07480    PTEN/MMAC1一种肿瘤抑制基因抗原       0.5mg/650             

QY-04200    PIBF（progesterone induced blocking factor）孕激素诱导阻断因子(抗原)         0.5mg/6501mg/1100               

QY-0665R    PIWIL1(piwi-like 1)piwi 样1蛋白(抗原)       0.5mg/6501mg/1100             

QY-05300    PPARγ (peroxisome proliferator-activated receptor-gamma)过氧化酶活化增生受体γ（抗原）      0.5mg/6501mg/1100             

QY-04900    PP2Ac蛋白磷酸酶4(抗原)      0.5mg/6501mg/1100             

QY-03780    Runx3一种新发现的抑癌基因(抗原)      0.5mg/6501mg/1100             

QY-02780    RRAD (Ras-related associated with diabetes )RRAD(多肽抗原)       0.5mg/6501mg/1100             

QY-07830    SDF-1/CXCL12 (stromal cell derived factor-1)基质细胞衍生因子-1(抗原)         0.5mg/6501mg/1100               

QY-02110    shank1(SH3 ａnd multiple anleyrin repead domins protein 1)shank1抗原      0.5mg/6501mg/1100             

QY-04440    slc22A17 (solute carrier famili 22)溶质转运蛋白家族22成员17(多肽抗原)       0.5mg/6501mg/1100               

QY-04960    SLC24A5 (solute carrier family 24, member 5)可溶性载质转运蛋白SLC24A5（抗原）        0.5mg/6501mg/1100             

QY-04730    solute carrier family 1溶质携带物家族-1(抗原)        0.5mg/6501mg/1100              【大鼠二肽基肽酶Ⅳ(DPPⅣ)ELISA试剂盒现货】

QY-04780    SLC6A4 (solute carrier family 6)钾依赖钠钙交换蛋白6(抗原)        0.5mg/6501mg/1100             

QY-06150    Survivin细胞凋亡抑制因子的一种（抗原）       0.5mg/6501mg/1100             

QY-07260    BIRC5/Survivin variant细胞凋亡抑制因子4（抗原）         0.5mg/6501mg/1100             

QY-06350    SSA/RO (ribonucleoprotein complex,RNP)核糖核蛋白抗原SSA(抗原)        0.5ml/6501mg/1100             

QY-02760    SSB/La(Sjogren syndrome type B antigen)(抗原)抗干燥症SSB/La蛋白抗体         0.5mg/6501mg/1100               

QY-07180    Smad2(Mothers against decapentaplegic homolog 2)Smad 2/3(抗原)       0.5mg/6501mg/1100             

QY-07170    SPAG5/map126 (sperm associated antigen 5）抗精子相关抗原5(抗原)      0.2ml/1280            

QY-07700    SP-A (pulmonary surfactant-associated glycoprotein A)肺表面活性蛋白A(抗原)       0.5mg/650             

QY-07700    SP-A (pulmonary surfactant-associated glycoprotein A)肺表面活性蛋白A(抗原)       0.5mg/6501mg/1100             

QY-06790    SYVN1(synovial apoptosis inhibitor 1)滑膜细胞凋亡抑制物1（抗体）         0.5mg/6501mg/1100             

QY-06850    SYK (Spleen tyrosine kinase)非受体型酪氨酸激酶(抗原)         0.5mg/6501mg/1100             

QY-04190    Tau protein微管相关蛋白（抗原）      0.5mg/6501mg/1100             

QY-06380    TGF-βR 1/ALK-5(TGF-beta receptor type I)转移生长因子–β受体 1（抗原）      0.5mg/6501mg/1100               

QY-07780    Thy-1/CD90CD90(抗原)         0.5mg/6501mg/1100             

QY-06320    Thioster-containing protein 1硫酯包含蛋白-1(抗原)        0.5mg/6501mg/1100             

QY-06400    TIGAR humen (TP53- induced glycolysis ａnd apoptosis-regulator )P53诱导糖酵解和凋亡调节因子蛋白（抗原）         0.5mg/6501mg/1100              【大鼠二肽基肽酶Ⅳ(DPPⅣ)ELISA试剂盒现货】

QY-04180    TIMP-4(Tissue Inhibitor of Metalloproteinase-4)金属蛋白酶组织抑制因子-4(抗原)        0.5mg/6501mg/1100             

QY-04470    TPO(Thyroid Peroxidase)甲状腺过氧化物酶(抗原)        0.5mg/6501mg/1100