齐一生物试剂盒组分经过重重优化，每个组分的稳定性都完美的经过高温破坏测验，程度的避免了长途运输和保存过程中抗原抗体的失活.齐一生物试剂盒凭借其完美的标曲.严格的质控.稳定高、易保存，操作简便，已广泛应用在全国免疫学检验研究所的各领域中.深受研究者们的青睐. 齐一生物销售：021－6034 8496；181214 53965；173021 04490

【大鼠前列腺酸性磷酸酶(PAP)ELISA试剂盒厂家】齐一生物试剂盒凭借其完美的标曲、严格的质控、较高的稳定性，在世界范围内的科研试剂领域中脱颖而出深受研究者们的青睐. 齐一生物销售：021－6034 8496；181214 53965；173021 04490

【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【大鼠前列腺酸性磷酸酶(PAP)ELISA试剂盒厂家】试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

操作注意事项：

1.试剂盒应按标签说明书储存，使用前恢复到室温30分钟后方可使用。稀稀过后的标准品应丢弃，不可保存。

2.实验中不用的板条应立即放回包装袋中，密封保存，以免变质。

3.不用的其它试剂应包装好或盖好。不同批号的试剂不要混用.请在试剂盒保质前使用。

4.使用一次性的吸头以免交叉污染，吸取终止液和底物A、B液时，避免使用带金属部分的加样器。

5.使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。

6.洗涤酶标板时应充分拍干，不要将吸水纸直接放入酶标反应孔中吸水。

7.底物A应挥发，避免长时间打开盖子。底物B对光敏感，避免长时间暴露于光下。避免用手接触，有毒。实验完成后应立即读取OD值。

8.加入试剂的顺序应一致，以保证所有反应板孔温育的时间一样。

9.按照说明书中标明的时间、加液的量及顺序进行温育操作。

【大鼠前列腺酸性磷酸酶(PAP)ELISA试剂盒厂家】齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

操作步骤
实验开始前，请提前配置好所有试剂，试剂或样品稀释时，均需混匀，混匀时尽量避免起泡。每次检测都应该做标准曲线。如样品浓度过高时，用样品稀释液进行稀释，以使样品符合试剂盒的检测范围。
1.         加样：分别设空白孔、标准孔、待测样品孔。空白孔加样品稀释液100μl，余孔分别加标准品或待测样品100μl，注意不要有气泡，加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀，酶标板加上盖或覆膜，37℃反应120分钟。
为保证实验结果有效性，每次实验请使用新的标准品溶液。
2.         弃去液体，甩干，不用洗涤。每孔加生物素标记抗体工作液 100μl（取1μl生物素标记抗体加99μl生物素标记抗体稀释液的比例配制，轻轻混匀，在使用前一小时内配制），37℃,60分钟。
3.         温育60分钟后，弃去孔内液体，甩干，洗板3次，每次浸泡1-2分钟，350μl/每孔，甩干。
4.         每孔加辣根过氧化物酶标记亲和素工作液（同生物素标记抗体工作液） 100μl，37℃，60分钟。
5.         温育60分钟后，弃去孔内液体，甩干，洗板5次，每次浸泡1-2分钟，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光显色（30分钟内，此时肉眼可见标准品的前3-4孔有明显的梯度蓝色，后3-4孔梯度不明显，即可终止）。
7.         依序每孔加终止溶液50μl，终止反应（此时蓝色立转黄色）。终止液的加入顺序应尽量与底物液的加入顺序相同。为了保证实验结果的准确性，底物反应时间到后应尽快加入终止液。
8.         用酶联仪在450nm波长依序测量各孔的光密度（OD值）。 在加终止液后15分钟以内进行检测。
【大鼠前列腺酸性磷酸酶(PAP)ELISA试剂盒厂家】注：
1. 用户在初次使用试剂盒时，应将各种试剂管离心数分钟，以便试剂集中到管底。
2. 每次实验留一孔作为空白调零孔，该孔不加任何试剂，只是后加底物溶液及2N H2SO4。测量时先用此孔调OD值至零。
3. 为防止样品蒸发，试验时将反应板放于铺有湿布的密闭盒内，酶标板加上盖或覆膜。
4. 未使用完的酶标板或者试剂，请于2-8℃保存。标准品、生物素标记抗体工作液、辣根过氧化物酶标记亲和素工作液请依据所需的量配置使用。请勿重复使用已稀释过的标准品、生物素标记抗体工作液或、辣根过氧化物酶标记亲和素工作液。
5. 建议检测样品时均设双孔测定，以保证检测结果的准确性。
以上资料仅供您参考，如有问题欢迎来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

QY-x1234    人前列腺癌细胞UI-1                     

QY-x1235    人前列腺癌细胞22RV1                    

QY-x1236    前列腺癌细胞LncaP                      

QY-x1237    非雄激素依赖型前列腺癌Tsu-Prl                      

QY-x1238    人胎盘绒毛癌细胞JAR                    

QY-x1239    人绒癌细胞JEG-3                    

QY-x1240    人绒毛膜癌JEG                      

QY-x1241    人胎盘绒膜癌细胞BeWo                       

QY-x1242    人绒癌细胞耐药株JEG-3/VP16                      【大鼠前列腺酸性磷酸酶(PAP)ELISA试剂盒厂家】

QY-x1243    白介素-2转染耐VP16绒癌细胞JEG-3/VP16-IL-2                     

QY-x1244    TNFa转染耐VP16绒癌细胞JEG-3-VP16-TNFa                     

QY-x1245    人前列腺癌细胞ALVA                     

QY-x1246    人前列腺癌细胞gpc-1                    

QY-x1247    人前列腺癌细胞9L-B                     

QY-x1248    人前列腺癌细胞9L-E                     

QY-x1249    人前列腺癌细胞JCA-1                    

QY-x1250    人前列腺癌细胞VLcop                    

QY-x1251    人前列腺上皮细胞RWPE1                      

QY-x1252    人前列腺上皮细胞RWPE2                      

QY-x1253    人男性正常龟头细胞系HS68                       

QY-x1254    小儿包皮细胞HFF                    

QY-x1255    人正常前列腺上皮细胞RWPE-1                     

QY-x1256    人慢性髓原白血病细胞K562                       

QY-x1257    人白血病阿霉素耐药株K562/Adr                       

QY-x1258    人原髓细胞白血病细胞HL60                        【大鼠前列腺酸性磷酸酶(PAP)ELISA试剂盒厂家】

QY-x1259    人白血病阿霉素耐药株HL-60/Ad                       

QY-x1260    人红白细胞白血病细胞ErythroleukemiaHEL                     

QY-x1261    人白血病细胞SK                     

QY-x1262    人急性早幼粒白血病细胞（悬浮）NB4                      

QY-x1263    人白血病细胞Daudi                      

QY-x1264    人白血病细胞KG-1                       

9236421 Switch, firmware loading, TWI       CNY            

9236359 Sheath Filter with Quick Disconnect     CNY            

9236346 1.5A Fuse LiquiChip     CNY            

9236338 Quick disconnect Set        CNY            

9236164 Syringe block, repl. kit, RapidPlate        CNY            

9236153 Locator Plate, Tips, pos 2, BR RP       CNY            

9235698 Cable 24V, RCS1     CNY            

9235296 Capillary, Dispenser head, Mounting Tool        CNY            

9235054 Incubator Tower (65°C) Board       CNY            

9234941 Incubator Tower (65°C) Board DINCS     CNY            

9238658 Cooling compressor T2178GK, 50Hz        CNY            

9242060 Adapter plate, IQ/OQ, QS        CNY            

9242026 Repair Thermo, Depot Repair, Palm Cycler        CNY            

9241824 Pyro Q96 MD, Full Agreement The Full Agreement, PyM provides full service coverage for the PyroMark instrument. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one on-site inspection service, with costs for labor ａnd parts covered. With a Full Agreement, there is a response time of 2 working days.   CNY            

9241815 Pyro Q96 ID, PM Agreement       CNY             【大鼠前列腺酸性磷酸酶(PAP)ELISA试剂盒厂家】

9241172 anti-rotation tool      CNY            

9240960 Cable, cLLD, pipetting system, Qsym     CNY            

9240949 Cable, y, portal, Qsym      CNY            

9240678 Bimba Rotary Actuator, AP       CNY            

9240777 CE Update, AP196/98     CNY            

9241773 RGQ, Installation   The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the Rotorgene-Q systems hardware ａnd trains the user on the operation of the Rotorgene software. After the training the user will be able to develop application programs, conduct a basic troubleshooting, ａnd understand the application output format.All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements.  CNY            

9241763 QIAgility, Full Agreement   "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) or PC repair provision: not included

Transportation cost coverage: not applicable"   CNY            

9241372 Reflector, UV lamp, EZ1a        CNY            

9243545 QIAsymphony SP, PM Service  "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY            

9243045 Bracket, rear, transparent panel, Qsym      CNY            

9242980 QIArent&go - Rent out fee QS RGQ System     CNY            

9242764 QS RGQ, Premium Agreement   "Repair service delivery QIAsymphony SP/AS: onsite

Service response time (repair) QIAsymphony: 24 hours/1 business day

Repair service delivery RGQ: send to Regional Repair Center

Service response time (repair) RGQ: 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision RGQ: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage

QIAsymphony Response Time starts when customer inquiry is received by local tech service ends when a service specialist is onsite.

- Inquiry Channel: Local Tech-Service Hotline, Mo.- Fr. 08:00-17:00 Uhr

- Call Cut-off Time: 12:00 am; on business days

- Customer Availability: maximum 2 hours phone trouble shooting to identify error ａnd spare parts

- QIAsense: Systems need to be connected to QIAsense for faster download of log files

- Software: QIAsymphony need to be updated with the latest software version

- Remote Area: 24 Hr onsite response only available for geographical areas within field service travel capability ａnd pre-12:00 delivery capability of spare parts"   CNY            

9242750 Cooling adapter, PCR adapter, AS, Qsym      CNY            

9242709 Validation Support, 2d, EZ1 The QIAGEN EZ1 Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the EZ1 system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, ａnd practical hands-on operation.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates. The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.    CNY            

9242699 QIAxcel, Full Agreement "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery:

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage"   CNY            

9242508 QIAsymphony SPAS, Premium Agreement "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: not applicable

- QIAsense: Systems should to be connected to QIAsense for faster download of log files"    CNY             【大鼠前列腺酸性磷酸酶(PAP)ELISA试剂盒厂家】

5000-1316   Rotary Shaker - Knob Locking        CNY            

9241365 Grid, UV lamp, EZ1a     CNY