价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供优质产品。

【大鼠抑制素A(INH-A)ELISA试剂盒厂家】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【大鼠抑制素A(INH-A)ELISA试剂盒厂家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【大鼠抑制素A(INH-A)ELISA试剂盒厂家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【大鼠抑制素A(INH-A)ELISA试剂盒厂家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

QY-x1690 人肺腺癌                                                       

QY-x1691 人肺癌细胞     SPC-A-1              肺癌         RPMI 1640 + 10% FBS              

QY-x1692 人肺癌细                                                       

QY-x1693 人非小细胞肺癌细胞     A549         CCL-185?  肺癌         RPMI 1640 + 10% NBCS           

QY-x1694 人肺癌细胞     GLC82                肺癌                           

QY-x1695 人小细胞肺癌细胞                                                       

QY-x1696 人肺鳞癌细胞         NCI-H446 HTB-171?          肺癌         RPMI 1640 + 10% NBCS           

QY-x1697 人肺鳞癌细胞         NC1-H460                  肺鳞癌     RPMI 1640 + 10% FBS              

QY-x1698 鼠Lewis肺腺癌细胞（非小细胞）      LLC            肺癌         RPMI 1640 + 10% FBS               【大鼠抑制素A(INH-A)ELISA试剂盒厂家】

QY-x1699 人胃腺癌细胞（低分化）     BGC-823            胃癌         RPMI 1640 + 10% NBCS           

QY-x1700 人胃癌细胞     NCI-N87   CRL-5822?       胃癌         RPMI 1640 + 10% FBS              

QY-x1701 人胃癌细胞（未分化）         HGC-27              胃癌         RPMI 1640 + 10% NBCS           

QY-x1702 人乳腺癌细胞         MCF-7       HTB-22?   腺癌，乳腺，胸水。     DMEM＋10％NBCS                  

QY-x1703 人乳腺癌细胞         MDA-MB-435S         HTB-129?          mammary gland; breast  DMEM＋10％NBCS         129&Template=cellBiology     

QY-x1704 人宫颈癌细胞         HeLa         CCL-2?      宫颈腺癌         DMEM＋10％NBCS                  

QY-x1705 人结肠癌细胞         CaCo-2      HTB-37?   结肠癌     DMEM＋10％FBS            

QY-x1706 人胰腺癌细胞         PANC-1     CRL-1469?        胰腺；胰管；上皮细胞癌     DMEM＋10％FBS            

QY-x1707 人恶性黑色素瘤细胞     A-375        CRL-1619?       皮肤；恶性黑色素瘤     DMEM＋10％NBCS                  

QY-x1708 鼠黑色素瘤细胞     B16-luc              黑色素瘤         RPMI 1640 + 10% NBCS           

QY-x1709 人纤维肉瘤细胞     HT1080    CCL-121?          纤维肉瘤         MEM或 DMEM＋10％FBS            

QY-x1710 人原髓细胞白血病细胞（悬浮）         HL-60        CCL-240?          外周血；早幼粒细胞；急性粒－单核细胞白血病         RPMI 1640 + 10% NBCS           

QY-x1711 人T淋巴细胞白血病细胞（悬浮）     Jurkat E6-1       TIB-152?  T淋巴细胞；急性T细胞白血病  RPMI 1640 + 10% FBS                  

QY-x1712 人T淋巴细胞白血病细胞株                                                       

QY-x1713 大鼠或人主动脉平滑稽细胞A10                                               

QY-x1714 大鼠肝细胞株BRL3A                                                    【大鼠抑制素A(INH-A)ELISA试剂盒厂家】

QY-x1715 人慢性髓原白血病细胞系     K562                            DMEM+10%NBCS            

QY-x1716 草地贪夜                                                       

QY-x1717 蛾细胞     sf-21                   蛾                      

QY-x1718 （昆虫）         S900                                              

QY-x1719 昆虫细胞         Sf-9            昆虫         S900                  

QY-x1720 鼠肝星状细胞         HSC           鼠胚胎     DMEM＋10％FBS            

9019465  Pads, D=10mm, white (85), Cxx                CNY                             

9019459  Transformer (115V), HS            CNY                             

9233429  Valve, magnet, dispenser, BR8000           CNY                             

9233334  Solenoid Valve, 5-fold                CNY                             

9237332  Install, BR plus 2 integr. w. scheduling     Installation ａnd training, BioRobot System including two approved integration devices with scheduling   CNY                             

9237167  Bottom Air Filter               CNY                             

9232994  Board, LED, BR8000                  CNY                             

9237158  RTD Adapter Board XYP           CNY                             

9237152  Power Inlet                CNY                             

9237108  Protocol Dev./Opt., on-site (4-days)         The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 4 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.          CNY                             

9237098  Upgrade QIAsoft, on-site                   CNY                             

9242092  QIAgility, Inspection Agreement               CNY                             

9236425  Motor, vertical, TWI                  CNY                             

9236423  Belt, rotary, TWI               CNY                             

9236413  Storage Pod TW II             CNY                              【大鼠抑制素A(INH-A)ELISA试剂盒厂家】

9232979  PCB, Main, Incubator                CNY                             

9232969  YZ 96-Connector Board 1 (Arm), E10102                   CNY                             

9236159  Motor, rotary, repl. kit, RapidPlate          CNY                             

9236143  RS232 Bypass Tool             CNY                             

9236108  Manual, User, English, Plato 3                   CNY                             

9235773  Reagent Holder, 2-trough, 20 ml     Holder for accommodating 2 disposable troughs. For use with Buffer AL ａnd ethanol on the BioRobot MDx   CNY                             

9230748  Stirrerbehälter Rose80             CNY                             

9020088  Power Unit 200W, TLyser 2               CNY                             

9239500  Shockmnt, 7/16D,1/2L,8-32x3/8 St,Neo(10)                CNY                             

9232755  MTP-Plate Holder 1.01             CNY                             

9239362  Inlet-micro-fuse T 6.3A, 250V, EZ1           CNY                             

9239290  Cooling Block , 96-tube, 0.2 ml, Type B             CNY                             

9236448  Travel Charge, zone C                CNY                             

9232721  Cooling block, 24-tube 1.5 ml  Holder for accommodating 24 tubes (1.5 ml) on the worktable      CNY                             

9232720  Channeling block               CNY                             

9236436  Service, external, 1 day            CNY                              【大鼠抑制素A(INH-A)ELISA试剂盒厂家】

9239151  Screw, pod fixation (to support), TWII              CNY                             

9232688  Disposal Bag Holder Cover, BR3000 & 9604             CNY