价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供优质产品。

【人类组织相容性复合体Ⅰ类相关基因A(MICA)ELISA试剂盒现货】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人类组织相容性复合体Ⅰ类相关基因A(MICA)ELISA试剂盒现货】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人类组织相容性复合体Ⅰ类相关基因A(MICA)ELISA试剂盒现货】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人类组织相容性复合体Ⅰ类相关基因A(MICA)ELISA试剂盒现货】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9013308  Base, vacuum manifold, v1.04                   CNY                             

9013298  Top plate, vacuum manifold96 plate , 4           CNY                             

9013236  Tubing set, vacuum, 200, 0               CNY                             

9013224  Gripper set, V-handler, BR3000                 CNY                             

9013197  Tubing, L1 outlet, auto vac,BR8000 v1.00                CNY                             

9011702  Cable, PCON98 to AC-box (level sensor),          CNY                             

9011681  Dispenser drive, BR8000          CNY                             

132635     NeXtal Stock Zinc chloride (50)        0,1M Zinc chloride - 50mL (4 x 12,5 ml tube)  CNY                             

132578     NeXtal Stock PEG 2,000 DME (50)   50% w/v Polyethylene glycol 2,000 dimethyl ether - 50mL (4 x 12,5 ml tube)         CNY                             

800341     Cosmid End-Sequencing, w/ prep              CNY                              【人类组织相容性复合体Ⅰ类相关基因A(MICA)ELISA试剂盒现货】

800308     siRNA Hairpin Run w/ prep                CNY                             

800121     Verification Sequencing, w/ prep     "Double-stranded sequencing of a given sequence

QIAGEN® DNA template preparation

Design ａnd synthesis of walking primers

Data assembly ａnd sequence alignment

Accuracy >99.99%" CNY                             

132528     NeXtal Stock CAPSO untit. (50)         0.5M CAPSO - Untitrated - 50mL (4 x 12,5 ml tube)         CNY                             

132510     NeXtal Stock ADA untit. (50)   0.5M ADA - Untitrated - 50mL (4 x 12,5 ml tube)   CNY                             

764114     PAXgene Bone Marrow RNA Tubes (50)           CNY                             

679913     ipsogen RT Kit (33)            CNY                             

203643     HotStarTaq Plus Master Mix Kit (250)     "For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."  CNY                             

201225     Taq PCR Core Kit (1000)   "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

201203     Taq DNA Polymerase (250)      "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

180922     GeneRead Adapter L Set 1plex (12)                   CNY                             

180912     GeneRead Adapter I Set 1plex (12)          CNY                             

159214     QIAcard FTA Indicator Four Spots (25)              CNY                             

159201     QIAcard FTA One Spot (100)              CNY                             

9235523  Needle cleaning assembly P/N 4567-01            CNY                             

9235300  Plunger tip, syringe 2,5ml                  CNY                             

9235117  Fuse F4A, v1.00                  CNY                              【人类组织相容性复合体Ⅰ类相关基因A(MICA)ELISA试剂盒现货】

9022818  EZ1 Advanced XL Flip Cap Tube Rack                 CNY                             

9234889  Vacuum manifold, 96 seal                  CNY                             

9234862  System liquid container (2l Nalgene)                 CNY                             

9239555  PCBA, Laser Driver, Unlimited          CNY                             

9238664  Hinge                 CNY                             

9238662  Lid with window                CNY                             

9238659  Cooling compressor T2168GK, 60Hz                  CNY                             

L-Glu         左旋谷氨酰胺溶液        L-Glutamine Solution, 200 mM        100 ml                                 

NEAA        100X非必需氨基酸        100X Non-Essential Amino Acids      100ml                                  

HGL PCR  人胚层检测试剂盒         Human Germ Layer Detection Kit   50 reactions                               

Ored         油红O染色试剂盒         Oil Red O Staining Kit       100 ml                                

StemDS    人胚胎干细胞分离液     StemDS® Human Embryonic Stem Cell Dissociation Solution  100 ml                                

BPF  牛血浆纤维粘连蛋白     Bovine Plasma Fibronectin       1 mg                                    

HCF  人细胞纤连蛋白     Human Cellular Fibronectin     100 μg                             

BPV  牛血浆玻连蛋白     Bovine Plasma Vitronectin    100 μg                             

rHSA         重组人血清白蛋白         "Recombinant Human Serum Albumin

"       1g                                

BPF  牛血浆纤维粘连蛋白     Bovine Plasma Fibronectin       1 mg                                    

CCCSCK     胶原I-细胞培养表面包被试剂盒        Collage I-Cell Culture Surface Coating Kit         10 mg                                  

PLL   多聚赖氨酸   Poly-L-Lysine 1 mg/ml       1 ml                            

PLL   多聚赖氨酸   Poly-L-Lysine 10 mg/ml    1 ml                             【人类组织相容性复合体Ⅰ类相关基因A(MICA)ELISA试剂盒现货】

STEMium 人胚胎干细胞无血清培养基         Human Pluripotent Stem Cell Growth Medium       500 ml                                 

STEMium-XF     无异种人胚胎干细胞生长培养基         Xeno-free Human Pluripotent Stem Cell Growth Medium       500 ml                                 

STEMium-ACF  无动物成分的人胚胎干细胞生长培养基     Animal Component-free Human Pluripotent Stem Cell Growth Medium         500 ml                                 

StemDS    人胚胎干细胞分离液     StemDS® Human Embryonic Stem Cell Dissociation Solution  100 ml                                

StemCryo 人胚胎干细胞冻存液     Human Pluripotent Stem Cell Cryopreservation Medium        50ml                                    

StemCryo 人胚胎干细胞冻存液     Human Pluripotent Stem Cell Cryopreservation Medium,       5 x 10mL                                     

MEF 小鼠胚胎成纤维细胞     Mouse Embryonic Fibroblasts 5 x 10^5 cells/vial                                

MEF-mt    小鼠胚胎成纤维细胞－－处理  Mouse Embryonic Fibroblasts-mitomicine treated 5 x 10^5 cells/vial                                

MEF-mt    小鼠胚胎成纤维细胞－－CD1小鼠C处理        Mouse Embryonic Fibroblasts- Mitomycin C treated from CD1 mouse 1 x 10^6 cells/vial                              

MEF-mt    小鼠胚胎成纤维细胞－－CF1小鼠C处理         Mouse Embryonic Fibroblasts from CF1 mouse      1 x 10^6 cells/vial                                

MEF-mt    小鼠胚胎成纤维细胞－－CF1小鼠C处理         Mouse Embryonic Fibroblasts-Mitomycin C treated from CF1 mouse    1 x 10^6 cells/vial                              

HDF-f-mt 人真皮成纤维细胞-胎儿C处理    Human Dermal Fibroblasts-Fetal-Mitomycin C treated  1 x 10^6 cells/vial                              

MEF-cm   小鼠胚胎成纤维细胞培养基         Mouse Embryonic Fibroblasts-Conditioned Medium        100 ml                                

bFGF-std MEF-cm     碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基         bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium     100ml                                  

ULBC plates      干细胞超低结合培养板         Ultra-Low Binding Culture Plate      6-well, 1 plate                                   

ULBC plates      超低结合培养板（6孔）      Ultra-Low Binding Culture Plate (6-well)         "6-well, 4 plates

"                                   【人类组织相容性复合体Ⅰ类相关基因A(MICA)ELISA试剂盒现货】

ULBC plate       超低结合培养板（24孔）    Ultra-Low Binding Culture Plate (24-well)      "24-well, 1 plate

"                                  

ULBC plates      超低结合培养板（24孔）    Ultra-Low Binding Culture Plate (24-well)      "24-well, 4 plates

"