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齐一生物现货供应人ELISA试剂盒，大鼠ELISA试剂盒，小鼠ELISA试剂盒等现货充足，购买任意ELISA试剂盒都享受免费代测服务，更多进口原装品牌试剂盒供应齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人白细胞介素-10（IL-10）ELISA试剂盒现货】酶法检测试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

【人白细胞介素-10（IL-10）ELISA试剂盒现货】操作注意事项：

1.试剂盒应按标签说明书储存，使用前恢复到室温30分钟后方可使用。稀稀过后的标准品应丢弃，不可保存。

2.实验中不用的板条应立即放回包装袋中，密封保存，以免变质。

3.不用的其它试剂应包装好或盖好。不同批号的试剂不要混用.请在试剂盒保质前使用。

4.使用一次性的吸头以免交叉污染，吸取终止液和底物A、B液时，避免使用带金属部分的加样器。

5.使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。

6.洗涤酶标板时应充分拍干，不要将吸水纸直接放入酶标反应孔中吸水。

7.底物A应挥发，避免长时间打开盖子。底物B对光敏感，避免长时间暴露于光下。避免用手接触，有毒。实验完成后应立即读取OD值。

8.加入试剂的顺序应一致，以保证所有反应板孔温育的时间一样。

9.按照说明书中标明的时间、加液的量及顺序进行温育操作。

【人白细胞介素-10（IL-10）ELISA试剂盒现货】齐一生物客户寄标本时需注明以下情况：

1、标本编号；2、所测项目；3、是否做复孔；3、联系方式；4、实验后标本是否寄回。

客户必知：

客户应对所提供的材料及信息负责，如因客户提供的材料及信息不准确而引起的实验延误或经济损失由客户承担。

齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

样本保存条件：

1.2-8℃冰箱多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人白细胞介素-10（IL-10）ELISA试剂盒现货】齐一生物供应，以上资料供参考如有疑问需要详细资料请联系我们客服人员，齐一生物销售：021－6034 8496；181214 53965；173021 04490

59445       EpiTect HRM PCR Kit (100)                CNY                             

381215     Investigator HDplex Kit (100)           CNY                             

4863004  pg NDV Univ. RT-PCR ASSAY LE                  CNY                             

4873004  pg AIV-H5 RT-PCR ASSAY           CNY                             

130923     NeXtal DWBlock Classics II Suite               CNY                             

130920     NeXtal DWBlock JCSG+ Suite            CNY                             

4621063  artus MTHFR LC PCR Kit (24) CE                CNY                             

4841014  pg HPV PCR ASSAY            CNY                             

4555265  artus M.tuberculosis RG PCR Kit (96) CE          CNY                             

4551065  artus Borrelia LC PCR Kit (96) CE               CNY                             

4513303  artus HI Virus-1 QS-RGQ Kit (24) RUO              CNY                             

4510033  artus Enterovirus LC RT-PCR Kit (24) PRE                   CNY                              【人白细胞介素-10（IL-10）ELISA试剂盒现货】

130718     NeXtal Tubes PACT Suite           CNY                             

122001     SeqTarget Primer Select (400)                   CNY                             

4506165  artus HBV TM PCR Kit (96) CE          CNY                             

120016     QIAquick 96 Plates (24)    Individual QIAquick 96 plates ａnd handbook. No buffers or reagents.    CNY                             

75162       RNeasy Maxi Kit (12)        12 RNeasy Maxi Spin Columns, Collection Tubes (50 ml), RNase-free Reagents ａnd Buffers  CNY                             

74704       RNeasy Fibrous Tissue Mini Kit (50)         50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), Proteinase K, RNase-free DNase I, RNase-free Reagents ａnd Buffers  CNY                             

27406       QuickLyse Miniprep Kit (250)            CNY                             

73504       RNeasy FFPE Kit (50)                  CNY                             

73442       RNeasy Plus Universal Tissue Midi Kit10          CNY                             

72041       Oligotex Direct mRNA Midi/Maxi Kit       For 6 mRNA midi or 2 maxipreps: 1 ml Oligotex Suspension, Large Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents ａnd Buffers  CNY                             

26173       R.E.A.L. Prep 96 Plasmid Kit (24)     For 24 x 96 rapid extraction alkaline lysis minipreps: 24 QIAfilter 96 Plates, Square-Well Blocks, Tape Pads, Reagents, Buffers      CNY                             

20051       QIAEX II Gel Extraction Kit (500)     For 500 extractions: 5 x 1.0 ml QIAEX II Suspension, Buffers  CNY                             

19743       QIAfilter Midi Cartridges (25)  25 QIAfilter Midi Cartridges     CNY                             

69982       TissueLyser Adapter Set (2 x 24)      2 sets of Adapter Plates ａnd 2 racks for use with 2.0 ml microcentrifuge tubes on the TissueLyser. Also contains 6 silicone sealing strips. (3 per adapter) to ensure 2-ml tubes are secure.    CNY                             

19413       QIAvac 24 Plus          CNY                             

19088       Reagent DX               CNY                              【人白细胞介素-10（IL-10）ELISA试剂盒现货】

19086       Buffer EB (250ml)    250 ml Elution Buffer       CNY                             

19073       Buffer AVL        "Buffer AVL (5x 31 ml, 250 preps)

(""QIAamp Viral Lysis Buffer, includes 5x 310 µg Carrier RNA (26.6 ug/ml), 270 Mini preps"")"    CNY                             

19051       Buffer P1 500 ml Resuspension Buffer (RNase A not included)        CNY                             

16181       QIAGEN Plasmid Plus 96 Miniprep  Kit (4)               CNY                             

12462       QIAGEN Large-Construct Kit (10)    10 QIAGEN-tip 500, Reagents, Buffers, ATP-Dependent Exonuclease    CNY                            

QT-00076 粪肠球菌(高耐)ATCC51299    冻干粉                                         

QT-00078 阪崎肠杆菌ATCC51329 冻干粉                                         

QT-00080 克柔念珠菌ATCC6258    冻干粉                                         

QT-00082 金黄色葡萄球菌ATCC6538    冻干粉                                         

QT-00084 枯草芽孢杆菌ATCC6633        冻干粉                                         

QT-00086 肺炎克雷伯菌ATCC700603    冻干粉                                         

QT-00088 热带念珠菌ATCC750      冻干粉                                         

QT-00090 嗜热脂肪地芽孢杆菌ATCC7953    冻干粉                                         

QT-00092 植物乳杆菌ATCC8014    冻干粉                                         

QT-00094 大肠埃希氏菌ATCC8739        冻干粉                                         

QT-00096 白色假丝酵母/白色念球菌ATCC90029         冻干粉                                          【人白细胞介素-10（IL-10）ELISA试剂盒现货】

QT-00100 铜绿假单胞杆菌（绿脓杆菌）ATCC9027     冻干粉                                         

QT-00102 枯草芽孢杆菌黑色变种ATCC9372        冻干粉                                         

QT-00103 艰难梭菌ClostridiumdifficileATCC9689  冻干粉                                         

QT-00104 啤酒酵母ATCC9763        冻干粉                                         

QT-00105 黑曲霉ATCC10582 冻干粉                                         

QT-00106 砖红链霉菌ATCC19776 冻干粉                                         

QT-00107 白色葡萄球菌ATCC8799        冻干粉                                         

QT-00108 皱褶青霉ATCC10128      冻干粉                                         

QT-00109 黑曲霉ATCC10864 冻干粉                                         

QT-00110 酿酒酵母ATCC26603      冻干粉                                         

QT-00111 酿酒酵母ATCC26785      冻干粉                                         

QT-00112 运动发酵单孢菌ATCC29191 冻干粉                                         

QT-00113 假单胞杆菌ATCC31554 冻干粉                                         

QT-00114 粪产碱杆菌ATCC31555 冻干粉                                         

QT-00115 菅囊酵母ATCC32691      冻干粉                                         

QT-00116 弗氏柠檬酸杆菌ATCC10787 冻干粉                                          【人白细胞介素-10（IL-10）ELISA试剂盒现货】

QT-00117 荚膜红细菌ATCC11166 冻干粉                                         

QT-00118 发根土壤杆菌（发根植物单胞菌）ATCC11325  冻干粉                                         

QT-00119 生孢梭菌（产芽孢梭状芽孢杆菌）ATCC11437  冻干粉                                         

QT-00120 痤疮丙酸杆菌ATCC11827      冻干粉                                         

　　测定小分子量物质常用竞争法，其标准曲线中吸光度与受检物质的浓度呈负相关。标准曲线的形状因试剂盒所用模式的差别而略有不同。ELISA测定的标准曲线注意图中横坐标为对数关系，这更有利于测定系统的表达。

        讲述完了以上ELISA实验的操作要点，可以发现其实很多步骤都并不复杂，重要的是操作的时候一定要注重细节，熟能生巧，才可以掌握ELISA实验的精髓所在。

133269     NeXtalStock Phosphate-citr. pH 4.2 (200)                 CNY                             

990522     Filter-Tips, 200 µl (960)            CNY                             

990396     QIAcube Modified Protocol               CNY                             

4501263  artus EBV RG PCR Kit (24) CE            CNY                             

4501205  artus EBV RG PCR Kit (96) RUO                 CNY                             

4500265  artus HSV-1/2 RG PCR Kit (96) CE               CNY                             

949008     Autopure Precipitation Soln (3800 ml)              CNY                             

335001     EpiTect Methyl qPCR Assay (200)              CNY                             

990332     Filter-Tips, 200 µl (1024)          CNY                             

939011     Buffer ATL ( 4 x 50 ml)               CNY                             

9010929  Motor, Y, with gearbox encoder                CNY                             

9010855  ZLV BGR DT-Abstreifer 4-fach Plato 7...D                  CNY                             

133246     NeXtalStock Sodium phosphate pH9.2 (200)            CNY                              【人细胞角蛋白18-M65（CK18-M65）ELISA试剂盒厂家】

330601     RT² SYBR Green FAST Mastermix (8)                 CNY                             

929604     QX Wash Buffer (40 ml)             CNY                             

9010810  Source rack (96 pos) for tubes 16mm x 1                   CNY                             

9010636  Microplate station, tall    Station for accommodating a 96-well plate (or other labware with the same base dimensions) on the worktable  CNY                             

69581       DNeasy 96 Blood & Tissue Kit (4)    For 4 x 96 DNA minipreps: 4 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers       CNY                             

63206       DyeEx 2.0 Spin Kit (250)  250 DyeEx Spin Columns, Collection Tubes (2 ml)    CNY                             

207043     QuantiTect Whole Transcriptome Kit (25)                 CNY                             

9010253  Gripper, right, landoplasted, MTP 120             CNY                             

979101     PyroMark Q96 HS Plate (100)  For sample analysis of DNA template on MD. CNY                             

979009     PyroMark Annealing Buffer (250 ml)        For annealing of sequencing primer to DNA template.   CNY                             

62124       EZ1 DSP DNA Blood Kit (48)     CE-IVD Certified Kit for Extraction of DNA from human whole Blood. To be used in combination with EZ1 DSP Instrument (SAP 9001360) ａnd EZ1 DSP Blood Card (SAP 9017713). Available Input Volumes 350 µl, selectable elution volumes 200 µl. Processing time for 6 samples 25 Minutes.       CNY                             

301709     HiPerFect Transfection Reagent (100 ml)                  CNY                             

9002199  RGQ 6plex, PC, PrioPLUS, no PM     "RGQ 6plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

Loaner available in 2 days*

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"          CNY                             

9002196  RGQ 2plex HRM, PC, PrioPLUS, basic,no PM   "RGQ 2plex HRM, PC Instrument

Installation of RGQ 2plex HRM & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Preventive Maintenance (RRC,  1 PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"          CNY                             

206546     Type-it HRM PCR Kit (2000)              CNY                             

206541     Rotor-Gene Type-it HRM Discovery Kit            CNY                              【人细胞角蛋白18-M65（CK18-M65）ELISA试剂盒厂家】

9012731  Diluent Trough + lid PK 80 white               CNY                             

9012721  syringe pump inlet tubing Plato 3100                CNY                             

978803     PyroMark OneStep RT-PCR Kit (200)                 CNY                             

133231     NeXtalStock Sodium cacodyl. pH 6.8 (100)                CNY                             

QT-00020 生孢梭菌ATCC19404      冻干粉                                         

QT-00022 破伤风杆菌/梭菌ClostridiumtetaniATCC19406     冻干粉                                         

QT-00024 化脓性链球菌ATCC19615      冻干粉                                         

QT-00025 黄杆菌属ATCC21047      冻干粉                                         

QT-00026 近平滑念珠菌ATCC22019      冻干粉                                         

QT-00027 变异链球菌ATCC25175 冻干粉                                         

QT-00028 脆弱拟杆菌ATCC25285 冻干粉                                         

QT-00029 洋葱假单胞菌ATCC25416      冻干粉                                         

QT-00030 大肠埃希氏菌ATCC25922      冻干粉                                         

QT-00032 金黄色葡萄球菌ATCC25923 冻干粉                                          【人细胞角蛋白18-M65（CK18-M65）ELISA试剂盒厂家】

QT-00034 宋内氏志贺氏菌(Sh.sonnei)ATCC25931 冻干粉                                         

QT-00035 金黄色葡萄球菌ATCC27217 冻干粉                                         

QT-00037 创伤弧菌ATCC27562      冻干粉                                         

QT-00038 铜绿假单胞杆菌（绿脓杆菌）ATCC27853  冻干粉                                         

QT-00040 粪肠球菌ATCC29212      冻干粉                                         

QT-00041 金黄色葡萄球菌ATCC29213 冻干粉                                         

QT-00047 两歧双歧杆菌ATCC29521      冻干粉                                         

QT-00049 多形拟杆菌ATCC29741 冻干粉                                         

QT-00051 粪肠球菌ATCC33186      冻干粉                                         

QT-00053 空肠弯曲菌ATCC33291 冻干粉                                         

QT-00055 金黄色葡萄球菌ATCC33591 冻干粉                                         

QT-00057 大肠埃希氏菌ATCC35218      冻干粉                                         

QT-00059 屎肠球菌ATCC35667      冻干粉                                         

QT-00061 变异链球菌ATCC35668 冻干粉                                         

QT-00063 迟缓真杆菌/迟缓埃格特菌ATCC43055         冻干粉                                         

QT-00065 金黄色葡萄球菌ATCC43300 冻干粉                                          【人细胞角蛋白18-M65（CK18-M65）ELISA试剂盒厂家】

QT-00067 弗氏柠檬酸杆菌ATCC43864 冻干粉                                         

QT-00069 淋病柰瑟氏菌ATCC49226      冻干粉                                         

QT-00071 流感嗜血杆菌ATCC49247      冻干粉                                         

QT-00072 肺炎链球菌ATCC49619 冻干粉                                         

QT-00074 流感嗜血杆菌ATCC49766      冻干粉