【人白细胞介素-8（IL-8）ELISA试剂盒厂家】齐一生物供应的ELISA试剂盒试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【人白细胞介素-8（IL-8）ELISA试剂盒厂家】齐一生物【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【人白细胞介素-8（IL-8）ELISA试剂盒厂家】齐一生物科技欢迎您订购咨询，齐一生物销售：021－6034 8496；181214 53965；173021 04490

齐一生物供应的ELISA试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人白细胞介素-8（IL-8）ELISA试剂盒厂家】齐一生物供应的ELISA试剂盒ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性. 【人白细胞介素-8（IL-8）ELISA试剂盒厂家】

以上资料仅供参考了解，不供于论文写作

齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490

12291       QIAfilter Plasmid Giga Kit (5)   5 QIAGEN-tip 10000, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges   CNY                            

133280     NeXtalStock TRICINE pH 7.5 (200)            CNY                             

4504005  artus Parvo B19 LC PCR Kit (96) RUO                CNY                             

9240726  Tube Assy, Jar/Pump, AP96/196                 CNY                             

9240725  Tube Assy, Fill, AP96/196           CNY                             

9240714  Contact Blade Kit, Capper, AP          CNY                             

L2172       hc2 GC-ID DNA Test PI (CD-ROM)             CNY                             

L2171       hc2 CT-ID HC2 DNA Test PI (CD-ROM)               CNY                             

12945       QIAGEN Plasmid Plus Midi Kit (100)         "100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges

 

QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."    CNY                             

9240704  Nipple, Suction Cup, AP             CNY                             

9240689  Jam nut, Shaker Bimba, AP98           CNY                              【人白细胞介素-8（IL-8）ELISA试剂盒厂家】

330540     Supplemental Microbial qPCR MM (Fluor)                CNY                             

208252     QuantiNova Probe PCR Kit (100)               CNY                             

288107     bactotype Mycoplasma Mg/Ms PCR Kit (480)                   CNY                             

1053393  Buffer RLT Plus (220 ml)   Please use this for replacement ａnd stand-alone selling CNY                             

274302     flocktype IBV Ab (2)          CNY                             

274012     flocktype AIV Ab (2)          CNY                             

272702     pigtype PRRSV Ab (2)                 CNY                             

273805     pigtype Yersinia Ab (20)            CNY                             

871101     EGFR PCR Kit (25)              CNY                             

9002244  QIAcube(220-240v) Promo, Priority         QIAcube (220-240V) Promo Priority includes the QIAcube instrument +promo iPad, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.        CNY                             

9018909  Rotor-Disc 100 Loading Block           CNY                             

9019520  ZLV ADAPTER KIT INVITEK                CNY                             

9020574  LED Assy, Green, 15" Long, DML 3000             CNY                             

9020599  Head Alignment Fixture, DML 3000                  CNY                             

9018042  Splash Guard, RCS1           CNY                             

9018190  Tool, arm parallelism, calibration, QIAC           CNY                              【人白细胞介素-8（IL-8）ELISA试剂盒厂家】

9022614  Ipad 3                CNY                             

9013384  Board, x-main, BR8000             CNY                             

9013248  BGR System Liquid            CNY                             

9013218  ZLV Screws, plastic, M6 (20)             CNY                             

9013217  ZLV Screws, plastic, M5 (20)             CNY                             

9013205  BGR Labhand li+re Digene                CNY                             

QT-00121 地衣芽孢杆菌ATCC11946      冻干粉                                         

QT-00122 谷氨酸棒杆菌ATCC13032      冻干粉                                         

QT-00123 熏衣草灰链霉菌ATCC13306 冻干粉                                         

QT-00124 猪霍乱沙门氏菌猪霍乱亚种ATCC13312      冻干粉                                         

QT-00125 脱氮假单胞菌ATCC13867      冻干粉                                         

QT-00126 耐放射异常球菌ATCC13939 冻干粉                                         

QT-00127 绿针假单胞菌ATCC13985      冻干粉                                         

QT-00128 肠炎沙门氏菌肠炎亚种ATCC14028      冻干粉                                         

QT-00129 巨大芽孢杆菌ATCC14581      冻干粉                                         

QT-00130 吸水链霉菌奥萨霉素亚种ATCC15420 冻干粉                                         

QT-00131 长双歧杆菌ATCC15707 冻干粉                                         

QT-00132 放射性根瘤菌ATCC15955      冻干粉                                          【人白细胞介素-8（IL-8）ELISA试剂盒厂家】

QT-00133 短密青霉ATCC16024      冻干粉                                         

QT-00134 副溶血性弧菌ATCC17802      冻干粉                                         

QT-00135 单核增生李斯特菌ATCC19115      冻干粉                                         

QT-00136 缺陷短波单胞菌ATCC19146 冻干粉                                         

QT-00137 师岗链霉菌ATCC19166 冻干粉                                         

QT-00138 脱氮付球菌ATCC19367 冻干粉                                         

QT-00139 丝状链霉菌ATCC19753 冻干粉                                         

QT-00140 裂殖壶菌ATCC20888      冻干粉                                         

QT-00141 产朊假丝酵母ATCC22023      冻干粉                                         

QT-00142 乙酸钙不动杆菌ATCC23055 冻干粉                                         

QT-00143 珊瑚链霉菌ATCC23901 冻干粉                                         

QT-00144 菲律宾链霉菌ATCC23905      冻干粉                                         

QT-00146 里氏木霉ATCC24449      冻干粉                                         

QT-00147 变异链球菌ATCC25175 冻干粉                                         

QT-00148 沙链霉菌ATCC25488      冻干粉                                         

QT-00149 酪丁酸梭菌ATCC25755 冻干粉                                          【人白细胞介素-8（IL-8）ELISA试剂盒厂家】

QT-00150 蛎灰链霉菌ATCC27455 冻干粉                                         

QT-00151 黄曲霉ATCC28539 冻干粉                                         

QT-00152 噬糖盐红菌ATCC29252 冻干粉                                         

m,�a-�/�x-� 生孢梭菌（产芽孢梭状芽孢杆菌）ATCC11437  冻干粉                                         

 

QT-00120 痤疮丙酸杆菌ATCC11827      冻干粉                                         

���`���/�x-���线的部分是理想的检测区域。

 

　　测定小分子量物质常用竞争法，其标准曲线中吸光度与受检物质的浓度呈负相关。标准曲线的形状因试剂盒所用模式的差别而略有不同。ELISA测定的标准曲线注意图中横坐标为对数关系，这更有利于测定系统的表达。

        讲述完了以上ELISA实验的操作要点，可以发现其实很多步骤都并不复杂，重要的是操作的时候一定要注重细节，熟能生巧，才可以掌握ELISA实验的精髓所在。

133269     NeXtalStock Phosphate-citr. pH 4.2 (200)                 CNY                             

990522     Filter-Tips, 200 µl (960)            CNY                             

990396     QIAcube Modified Protocol               CNY                             

4501263  artus EBV RG PCR Kit (24) CE            CNY                             

4501205  artus EBV RG PCR Kit (96) RUO                 CNY                             

4500265  artus HSV-1/2 RG PCR Kit (96) CE               CNY                             

949008     Autopure Precipitation Soln (3800 ml)              CNY                             

335001     EpiTect Methyl qPCR Assay (200)              CNY                             

990332     Filter-Tips, 200 µl (1024)          CNY                             

939011     Buffer ATL ( 4 x 50 ml)               CNY                             

9010929  Motor, Y, with gearbox encoder                CNY                             

9010855  ZLV BGR DT-Abstreifer 4-fach Plato 7...D                  CNY                             

133246     NeXtalStock Sodium phosphate pH9.2 (200)            CNY                              【人细胞角蛋白18-M65（CK18-M65）ELISA试剂盒厂家】

330601     RT² SYBR Green FAST Mastermix (8)                 CNY                             

929604     QX Wash Buffer (40 ml)             CNY                             

9010810  Source rack (96 pos) for tubes 16mm x 1                   CNY                             

9010636  Microplate station, tall    Station for accommodating a 96-well plate (or other labware with the same base dimensions) on the worktable  CNY                             

69581       DNeasy 96 Blood & Tissue Kit (4)    For 4 x 96 DNA minipreps: 4 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers       CNY                             

63206       DyeEx 2.0 Spin Kit (250)  250 DyeEx Spin Columns, Collection Tubes (2 ml)    CNY                             

207043     QuantiTect Whole Transcriptome Kit (25)                 CNY                             

9010253  Gripper, right, landoplasted, MTP 120             CNY                             

979101     PyroMark Q96 HS Plate (100)  For sample analysis of DNA template on MD. CNY                             

979009     PyroMark Annealing Buffer (250 ml)        For annealing of sequencing primer to DNA template.   CNY                             

62124       EZ1 DSP DNA Blood Kit (48)     CE-IVD Certified Kit for Extraction of DNA from human whole Blood. To be used in combination with EZ1 DSP Instrument (SAP 9001360) ａnd EZ1 DSP Blood Card (SAP 9017713). Available Input Volumes 350 µl, selectable elution volumes 200 µl. Processing time for 6 samples 25 Minutes.       CNY                             

301709     HiPerFect Transfection Reagent (100 ml)                  CNY                             

9002199  RGQ 6plex, PC, PrioPLUS, no PM     "RGQ 6plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

Loaner available in 2 days*

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"          CNY                             

9002196  RGQ 2plex HRM, PC, PrioPLUS, basic,no PM   "RGQ 2plex HRM, PC Instrument

Installation of RGQ 2plex HRM & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Preventive Maintenance (RRC,  1 PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"          CNY                             

206546     Type-it HRM PCR Kit (2000)              CNY                             

206541     Rotor-Gene Type-it HRM Discovery Kit            CNY                              【人细胞角蛋白18-M65（CK18-M65）ELISA试剂盒厂家】

9012731  Diluent Trough + lid PK 80 white               CNY                             

9012721  syringe pump inlet tubing Plato 3100                CNY                             

978803     PyroMark OneStep RT-PCR Kit (200)                 CNY                             

133231     NeXtalStock Sodium cacodyl. pH 6.8 (100)                CNY                             

QT-00020 生孢梭菌ATCC19404      冻干粉                                         

QT-00022 破伤风杆菌/梭菌ClostridiumtetaniATCC19406     冻干粉                                         

QT-00024 化脓性链球菌ATCC19615      冻干粉                                         

QT-00025 黄杆菌属ATCC21047      冻干粉                                         

QT-00026 近平滑念珠菌ATCC22019      冻干粉                                         

QT-00027 变异链球菌ATCC25175 冻干粉                                         

QT-00028 脆弱拟杆菌ATCC25285 冻干粉                                         

QT-00029 洋葱假单胞菌ATCC25416      冻干粉                                         

QT-00030 大肠埃希氏菌ATCC25922      冻干粉                                         

QT-00032 金黄色葡萄球菌ATCC25923 冻干粉                                          【人细胞角蛋白18-M65（CK18-M65）ELISA试剂盒厂家】

QT-00034 宋内氏志贺氏菌(Sh.sonnei)ATCC25931 冻干粉                                         

QT-00035 金黄色葡萄球菌ATCC27217 冻干粉                                         

QT-00037 创伤弧菌ATCC27562      冻干粉                                         

QT-00038 铜绿假单胞杆菌（绿脓杆菌）ATCC27853  冻干粉                                         

QT-00040 粪肠球菌ATCC29212      冻干粉                                         

QT-00041 金黄色葡萄球菌ATCC29213 冻干粉                                         

QT-00047 两歧双歧杆菌ATCC29521      冻干粉                                         

QT-00049 多形拟杆菌ATCC29741 冻干粉                                         

QT-00051 粪肠球菌ATCC33186      冻干粉                                         

QT-00053 空肠弯曲菌ATCC33291 冻干粉                                         

QT-00055 金黄色葡萄球菌ATCC33591 冻干粉                                         

QT-00057 大肠埃希氏菌ATCC35218      冻干粉                                         

QT-00059 屎肠球菌ATCC35667      冻干粉                                         

QT-00061 变异链球菌ATCC35668 冻干粉                                         

QT-00063 迟缓真杆菌/迟缓埃格特菌ATCC43055         冻干粉                                         

QT-00065 金黄色葡萄球菌ATCC43300 冻干粉                                          【人细胞角蛋白18-M65（CK18-M65）ELISA试剂盒厂家】

QT-00067 弗氏柠檬酸杆菌ATCC43864 冻干粉                                         

QT-00069 淋病柰瑟氏菌ATCC49226      冻干粉                                         

QT-00071 流感嗜血杆菌ATCC49247      冻干粉                                         

QT-00072 肺炎链球菌ATCC49619 冻干粉                                         

QT-00074 流感嗜血杆菌ATCC49766      冻干粉