检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人血管内皮细胞生长因子受体3(VEGFR-3/Flt-4)ELISA试剂盒现货】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人血管内皮细胞生长因子受体3(VEGFR-3/Flt-4)ELISA试剂盒现货】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人血管内皮细胞生长因子受体3(VEGFR-3/Flt-4)ELISA试剂盒现货】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【人血管内皮细胞生长因子受体3(VEGFR-3/Flt-4)ELISA试剂盒现货】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

QY-x1136 人胚肾细胞2V6.11                                                       

QY-x1137 人胚肾二倍体细胞CCC-HEK-1                                                     

QY-x1138 人肾癌细胞系(769-P)769-P                                                  

QY-x1139 人肾透明细胞癌皮肤转移细胞Caki-1                                               

QY-x1140 人肾上腺皮质腺癌细胞SW-13                                                    

QY-x1141 人肾癌细胞系A498                                                      

QY-x1142 人肾癌细胞系ACHN                                                    

QY-x1143 人肾癌细胞GRC-1                                               

QY-x1144 人肾上皮细胞GES                                               

QY-x1145 人肾癌Wilms细胞G401                                                      

QY-x1146 Asp2人胚胎肾细胞转化细胞FC33                                                       【人血管内皮细胞生长因子受体3(VEGFR-3/Flt-4)ELISA试剂盒现货】

QY-x1147 人肾癌细胞SK-RC-42                                                   

QY-x1148 人膀胱癌细胞EJ                                                  

QY-x1149 人膀胱癌细胞5637（HTB-9）                                                    

QY-x1150 人膀胱移行细胞癌细胞T24                                               

QY-x1151 人膀胱鳞癌细胞ScaBER                                                      

QY-x1152 人膀胱癌细胞BIU－87                                               

QY-x1153 人胆管癌细胞RBE                                              

QY-x1154 人胆管癌细胞QBC939                                                

QY-x1155 人乳腺癌细胞MCF-7                                                   

QY-x1156 人乳腺癌细胞MCF-7(NEW)                                                 

QY-x1157 人乳腺癌细胞MCF-7(OLD)                                                  

QY-x1158 人乳腺癌细胞MCF-7/ER36                                                  

QY-x1159 人乳腺癌细胞(耐阿霉素)MCF-7/ADR                                                  

QY-x1160 人乳腺癌细胞MCF-7B                                                 

QY-x1161 人乳腺癌细胞SKBr-2HL                                                       

QY-x1162 人乳腺癌细胞ZR75-1                                                   【人血管内皮细胞生长因子受体3(VEGFR-3/Flt-4)ELISA试剂盒现货】

QY-x1163 人乳腺癌细胞ZR75-30                                                

QY-x1164 人乳腺癌细胞MDA-MB-231                                               

QY-x1165 人乳腺癌细胞MDA-MB-468                                               

QY-x1166 人乳腺癌细胞Hs-578T                                                

QY-x1167 人乳腺癌细胞HCC1937                                              

QY-x1168 人乳腺癌细胞MDA-MB-415                                               

9239854  Sample holder Small, 1.2ml , v2.0             CNY                             

9239560  Assy, Optics with Coherent Laser              CNY                             

9239559  PowerSupply,ChsMt,100W,4DP,Coherent                 CNY                             

9020026  MORG sensor, 240mm, EZ1a            CNY                             

9020025  ZEL sensor, 390mm, EZ1a                  CNY                             

9019989  Transformer, CAS               CNY                             

9019980  Cover Kit VPT, PyroQ96VW               CNY                             

9019973  Square Silicon Foam, GSC                   CNY                             

9019968  Accessories for balance, IQ/OQ, QS                  CNY                             

9019937  Service kit, pipettor 8ch, Cx8            CNY                             

9019501  GEL LOADER ASSY GS                CNY                             

9019478  GEL CRACKER GS               CNY                             

9238887  Cable, Heat, BS96             CNY                              【人血管内皮细胞生长因子受体3(VEGFR-3/Flt-4)ELISA试剂盒现货】

9238835  #BioSprint15, Basic Agreement, no PM  The Basic Cover Agreement BioSprint 15 provides full service coverage for the BioSprint 15. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Cover Agreement, the response time is 5 working days.    CNY                             

9241216  Full Agreement Depot Repair, DCR-1, 1yr       "The Full Agreement Depot Repair, DCR-1 provides full service coverage for the DCR-1 luminometer used in the digene HPV application. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9241184  Sensor, Z, with cable, QX          CNY                             

9241181  Holder, sample plate, QX                   CNY                             

9238834  #Basic Cover Agreement 9604, 2PM, 1yr        The Basic Cover Agreement 9604, 2 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes two annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9237176  Bar Code Reader               CNY                             

9014514  X-Drive, Complete, BR8, RD              CNY                             

9014501  Vertical Tip Disposal Slide                  CNY                             

9022324  Power Supply, Shaker, careHPV                 CNY                             

9022322  Power Supply, Controller, careHPV           CNY                             

9022121  Tool. Rotor Hub Height Jig, RGQ               CNY                             

9021404  RGQ MDx User manuals for IVD use(Europe)           CNY                             

9238610  Collet chuck               CNY                             

9238479  Motor, syringe, RapidPlate               CNY                             

9238152  Elution Microplate Adapter 8000, SBS              CNY                             

9237932  Syringe, 100µl           CNY                              【人血管内皮细胞生长因子受体3(VEGFR-3/Flt-4)ELISA试剂盒现货】

9240663  Nut, Flangeless,1/4-28, Black, AP              CNY                             

9240634  Power Supply, Main PCB, Aztec, AP196/98                CNY