检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人血管内皮细胞生长因子受体1(VEGFR-1/Flt1)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

【人血管内皮细胞生长因子受体1(VEGFR-1/Flt1)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

【人血管内皮细胞生长因子受体1(VEGFR-1/Flt1)ELISA试剂盒厂家】注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

【人血管内皮细胞生长因子受体1(VEGFR-1/Flt1)ELISA试剂盒厂家】计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

QY-x1169 人乳腺癌高转移细胞MDA-MB-435                                                   

QY-x1170 人乳腺癌细胞MDA-MB-435S                                                      

QY-x1171 人乳腺癌细胞MDA-MB-436                                               

QY-x1172 人乳腺导管癌细胞MDA-MB-453                                               

QY-x1173 人乳腺细胞HBL-100                                                    

QY-x1174 人乳腺癌细胞BCaP-37                                                

QY-x1175 人乳腺癌细胞LCC1                                                      

QY-x1176 人乳腺癌细胞MX-1                                                      

QY-x1177 人乳腺管癌细胞T47D                                                 

QY-x1178 人乳腺癌细胞SK-BR-3                                                  【人血管内皮细胞生长因子受体1(VEGFR-1/Flt1)ELISA试剂盒厂家】

QY-x1179 人乳腺癌细胞4T1                                               

QY-x1180 人乳腺癌细胞HCC1937                                              

QY-x1181 人乳腺导管癌细胞HCC38                                                   

QY-x1182 人乳腺癌细胞Hs-578T                                                

QY-x1183 人乳腺癌Tamoxifen耐药株LCC2                                               

QY-x1184 人乳腺癌抗雌激素耐药株LCC9                                                  

QY-x1185 人乳腺癌阿霉素耐药细胞株MCF/Adr                                               

QY-x1186 人乳腺癌细胞BT-549                                                  

QY-x1187 人乳腺导管瘤细胞BT-474                                                  

QY-x1188 人乳腺癌细胞MB-271                                                

QY-x1189 人乳腺癌细胞ER-30                                                    

QY-x1190 人乳腺癌细胞M453                                                    

QY-x1191 人乳腺浸润性导管癌旁皮肤细胞CCD-1095Sk                                                  

QY-x1192 人乳腺细胞HBL-100                                                    

QY-x1193 人乳腺导管瘤细胞UACC812                                                       

QY-x1194 人乳腺癌细胞系1590                                                  【人血管内皮细胞生长因子受体1(VEGFR-1/Flt1)ELISA试剂盒厂家】

QY-x1195 人卵巢癌细胞A2780                                                   

QY-x1196 人卵巢癌细胞Sw626                                                   

QY-x1197 人卵巢癌顺铂耐药株COC1/CDDP                                              

QY-x1198 人卵巢癌紫杉醇耐药株A2780/Taxol                                                 

QY-x1199 人卵巢癌细胞系Anglne                                                       

QY-x1200 人卵巢癌细胞OVCaR-3                                               

QY-x1201 人卵巢癌细胞Caov-3                                                   

9240630  Power Supply, 5VDC 1.5 AMP, AP96                  CNY                             

9240593  Unload Valve, Pump, Pressure, AP196/98                  CNY                             

9240562  Fuse, PCB, 315, Eppendorf, AP196/98                CNY                             

9240536  Motor Assy, SiNug, 17, All, AP96/196                 CNY                             

9240514  CD R/W, AP196/98             CNY                             

9240504  PC-104, AP98            CNY                             

9240346  Autopure, Full Agreement       "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9240098  Re-Installation, BR EZ1    The Re-Installation, BR EZ1 facilitates quick implementation of system back into lab routine by minimizing  instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9239852  Foot bumpers,  v2.0 (4)          CNY                             

9019944  Cable, P-axis, wires, 8 ch. , C18                 CNY                             

9238880  Bearings up. & low, BS96                   CNY                             

9238836  BioSprint96, Basic Agreement, no PM    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: not applicable"        CNY                             

9241211  Full Agreement Depot Repair, DML2000, 1y  "The Full Agreement Depot Repair, DML2000 provides full service coverage for the DML 2000 luminometer. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9241191  Magnet, tool, QX              CNY                              【人血管内皮细胞生长因子受体1(VEGFR-1/Flt1)ELISA试剂盒厂家】

9238825  Spare bucket for plate rotor             CNY                             

9238815  Rect. carrier for 12 tubes 10 ml w/ cap            CNY                             

9019472  TAPE SPACER CLEAR GS             CNY                             

9019464  UV tube, Cxx             CNY                             

9243650  Comprehen Valid., EZ1/QIAcube/QIAsymph    """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

9237333  Install, BR plus 3 integr. w. scheduling     Installation ａnd training, BioRobot System including three approved integration devices with scheduling   CNY                             

9237328  Upgrade, BR & 2 ext. devices w. sched.  Upgrade BioRobot with two approved integration devices using scheduling software  CNY                             

9237173  Shield, V-housing               CNY                             

9233208  Tubing, liquid system 2.5/1.5             CNY                             

9237155  V-Housing with Sample Needle                  CNY                             

9237150  Heater Top                CNY                             

9016151  #App. Package, M48, Pathology               CNY                             

9019179  Opto - plate lock closed, PyroQ96MD               CNY                             

9011883  Upgrade, high speed pipetting system             CNY                             

9011882  Back unit, BR9600            CNY                             

9011823  Z-Modul; Tip-Adapter 300µl; BR8000               CNY                              【人血管内皮细胞生长因子受体1(VEGFR-1/Flt1)ELISA试剂盒厂家】

9236916  3 Year Warranty, LiquiChip       LiquiChip 3-year Warranty with 1 Preventive Maintenance per year.  Included in system price.  This warranty must be included as a line item on the system quote.  It covers the system for three years total including all per incident travel ａnd labor charges, all repair parts, one preventive maintenance visits per year (three in total), ａnd 48- hour priority response.     CNY