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齐一生物现货供应人ELISA试剂盒，大鼠ELISA试剂盒，小鼠ELISA试剂盒等现货充足，购买任意ELISA试剂盒都享受免费代测服务，更多进口原装品牌试剂盒供应齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人巨噬细胞集落刺激因子(M-CSF)ELISA试剂盒现货】酶法检测试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

【人巨噬细胞集落刺激因子(M-CSF)ELISA试剂盒现货】操作注意事项：

1.试剂盒应按标签说明书储存，使用前恢复到室温30分钟后方可使用。稀稀过后的标准品应丢弃，不可保存。

2.实验中不用的板条应立即放回包装袋中，密封保存，以免变质。

3.不用的其它试剂应包装好或盖好。不同批号的试剂不要混用.请在试剂盒保质前使用。

4.使用一次性的吸头以免交叉污染，吸取终止液和底物A、B液时，避免使用带金属部分的加样器。

5.使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。

6.洗涤酶标板时应充分拍干，不要将吸水纸直接放入酶标反应孔中吸水。

7.底物A应挥发，避免长时间打开盖子。底物B对光敏感，避免长时间暴露于光下。避免用手接触，有毒。实验完成后应立即读取OD值。

8.加入试剂的顺序应一致，以保证所有反应板孔温育的时间一样。

9.按照说明书中标明的时间、加液的量及顺序进行温育操作。

【人巨噬细胞集落刺激因子(M-CSF)ELISA试剂盒现货】齐一生物客户寄标本时需注明以下情况：

1、标本编号；2、所测项目；3、是否做复孔；3、联系方式；4、实验后标本是否寄回。

客户必知：

客户应对所提供的材料及信息负责，如因客户提供的材料及信息不准确而引起的实验延误或经济损失由客户承担。

齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

样本保存条件：

1.2-8℃冰箱多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人巨噬细胞集落刺激因子(M-CSF)ELISA试剂盒现货】齐一生物供应，以上资料供参考如有疑问需要详细资料请联系我们客服人员，齐一生物销售：021－6034 8496；181214 53965；173021 04490

QY-x2152 CHO-K1中国仓鼠卵巢细胞                                                

QY-x2153 ES-2人卵巢透明细胞癌细胞系                                                 

QY-x2154 H460人大细胞肺癌细胞系                                               

QY-x2155 HeLa 人宫颈癌细胞系                                                        

QY-x2156 HeLa 229人宫颈癌细胞系                                                 

QY-x2157 HL-60人髓性白血病细胞系（早幼粒白血病）                                                        

QY-x2158 Jurkat 人白血病T细胞系                                                  

QY-x2159 K-562人类红白血病细胞系                                                        

QY-x2160 MC3T3-E1小鼠成骨细胞                                                     

QY-x2161 A498人肾癌细胞                                                

QY-x2162 McCoy小鼠成纤维细胞                                                       【人巨噬细胞集落刺激因子(M-CSF)ELISA试剂盒现货】

QY-x2163 MCF-7人乳腺癌细胞系                                                       

QY-x2164 MDA-MB-231人乳腺癌细胞系                                                  

QY-x2165 MDCK 犬上皮细胞                                                      

QY-x2166 NIH-3T3小鼠成纤维细胞                                                   

QY-x2167 OVCAR-3人卵巢癌细胞系                                                  

QY-x2168 Raji人B淋巴细胞瘤                                                  

QY-x2169 SKOV3卵巢癌细胞系                                                  

QY-x2170 Vero 非洲绿猴肾细胞                                               

QY-x2171 Vero E6非洲绿猴肾细胞                                                    

QY-x2172 BJ 人皮肤成纤维细胞                                                 

QY-x2173 MGC-803 人胃癌细胞系                                                    

QY-x2174 SGC-7901 人胃癌细胞系                                                    

QY-x2175 THC-8307 人高分化结肠腺癌细胞系                                                        

QY-x2176 MIAPaca-2 胰腺癌细胞系                                                 

QY-x2177 293A 人胚肾细胞系                                                    

QY-x2178 3AO 人卵巢癌细胞系                                                 【人巨噬细胞集落刺激因子(M-CSF)ELISA试剂盒现货】

QY-x2179 M14 人肺癌细胞                                                 

QY-x2180 M21黑色素瘤细胞                                                       

QY-x2181 OMDA231人乳腺癌细胞                                                     

QY-x2182 HTB133人乳腺癌细胞                                                

QY-x2183 Caski 宫颈癌细胞                                                

QY-x2184 BJAB 人B细胞淋巴瘤细胞株                                                   

9013236  Tubing set, vacuum, 200, 0               CNY                             

9013224  Gripper set, V-handler, BR3000                 CNY                             

9013197  Tubing, L1 outlet, auto vac,BR8000 v1.00                CNY                             

9011702  Cable, PCON98 to AC-box (level sensor),          CNY                             

9011681  Dispenser drive, BR8000          CNY                             

132635     NeXtal Stock Zinc chloride (50)        0,1M Zinc chloride - 50mL (4 x 12,5 ml tube)  CNY                             

132578     NeXtal Stock PEG 2,000 DME (50)   50% w/v Polyethylene glycol 2,000 dimethyl ether - 50mL (4 x 12,5 ml tube)         CNY                             

800341     Cosmid End-Sequencing, w/ prep              CNY                             

800308     siRNA Hairpin Run w/ prep                CNY                             

800121     Verification Sequencing, w/ prep     "Double-stranded sequencing of a given sequence

QIAGEN® DNA template preparation

Design ａnd synthesis of walking primers

Data assembly ａnd sequence alignment

Accuracy >99.99%" CNY                             

132528     NeXtal Stock CAPSO untit. (50)         0.5M CAPSO - Untitrated - 50mL (4 x 12,5 ml tube)         CNY                             

132510     NeXtal Stock ADA untit. (50)   0.5M ADA - Untitrated - 50mL (4 x 12,5 ml tube)   CNY                             

764114     PAXgene Bone Marrow RNA Tubes (50)           CNY                              【人巨噬细胞集落刺激因子(M-CSF)ELISA试剂盒现货】

679913     ipsogen RT Kit (33)            CNY                             

203643     HotStarTaq Plus Master Mix Kit (250)     "For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."  CNY                             

201225     Taq PCR Core Kit (1000)   "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

201203     Taq DNA Polymerase (250)      "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

180922     GeneRead Adapter L Set 1plex (12)                   CNY                             

180912     GeneRead Adapter I Set 1plex (12)          CNY                             

159214     QIAcard FTA Indicator Four Spots (25)              CNY                             

159201     QIAcard FTA One Spot (100)              CNY                             

9235523  Needle cleaning assembly P/N 4567-01            CNY                             

9235300  Plunger tip, syringe 2,5ml                  CNY                             

9235117  Fuse F4A, v1.00                  CNY                             

9022818  EZ1 Advanced XL Flip Cap Tube Rack                 CNY                             

9234889  Vacuum manifold, 96 seal                  CNY                             

9234862  System liquid container (2l Nalgene)                 CNY                             

9239555  PCBA, Laser Driver, Unlimited          CNY                             

9238664  Hinge                 CNY                              【人巨噬细胞集落刺激因子(M-CSF)ELISA试剂盒现货】

9238662  Lid with window                CNY                             

9238659  Cooling compressor T2168GK, 60Hz                  CNY