【人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒厂家】齐一生物供应的ELISA试剂盒试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

【人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒厂家】齐一生物【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

ELISA应用：

ELISA检测试剂盒、ELISAKit、酶联免疫试剂盒、酶联免疫吸附测定试剂盒、酶联免疫分析试剂盒、酶免试剂盒等,比较常见的叫法是ELISA检测试剂盒、酶联免疫吸附测定试剂盒等ELISA试剂盒自从60-70年代问世以来,得到全世界科研工作者的认可及推崇,在欧美及中国获得很大的推广,尤其是国内生化领域的长足发展.Elisa生物试验是一种敏感性高,特异性强,重复性好的实验诊断方法.由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检验的各领域中.ELISA检测试剂盒适用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒IgM抗体ELISA检测

【人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒厂家】齐一生物科技欢迎您订购咨询，齐一生物销售：021－6034 8496；181214 53965；173021 04490

齐一生物供应的ELISA试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

样本保存条件：

1.2-8℃冰箱多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒厂家】齐一生物供应的ELISA试剂盒ELISA实验原理

试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测.先将生物素标记的抗体同时温育.洗涤后,加入亲和素标记过的HRP.再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用.产生颜色.颜色的深浅和样品中的浓度呈比例关系.

免疫测定技术的基础在于抗原抗体之间的特异结合反应,所以任何优质的诊断试剂离不开优质的原料,如抗原抗体,酶等.以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备.近年来,随着分子生物学的发展,使用基因工程方法制备各种特殊的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现.

HBsAg试剂特点（检测模式：临床抗体夹心法）：包被抗体为山羊多抗.多抗具有高亲和性和对抗原各种表位的反应性.酶表抗体为与HBsAg有不同结合位点的鼠复合单位,可测得HBsAg变异样品,提高检测的特异性（99.98%）提高检测的灵敏度,应用ParlEhrich学说（PEI）HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml

ELISA检测试剂盒应用定性夹心免疫检测技术,用合成的HEV多肽抗原包被微孔板板条,这些多肽是中国型HEV毒株核心氨基酸序列中抗原性很强的肽段,分别来自于该毒株的开放阅读框2和开放阅读框3.将样品或标准品加入孔中并孵育,如果其中存在HEVIgM抗体,这些抗体就会与HEV的多肽抗原结合,并固定在上面,洗板除去其它非特异性抗体和样品中的其它成份.然后加入羊抗人IgM-HRP（辣根过氧化物酶）酶结合物,经第二次孵育后,酶结合物就会与次孵育结合上的HEVIgM抗体相结合,洗板除去未结合的酶结合物,加入TMB底物溶液,在第三次孵育时会发生酶-底物反应,只有那些含有HEVIgM抗体和酶结合物所形成的复合物的孔才会发生颜色变化,加入硫酸溶液终止酶和底物间的反应,并在波长:450nm处测量O.D.值,按照本HEVIgM抗体elisa试剂盒的测试标准,O.D.值大于或等于Cut-Off值的样品被认为是初试阳性. 【人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒厂家】

以上资料仅供参考了解，不供于论文写作

齐一生物专业为您提供高品质，高灵敏度.高质量的酶联免疫试剂盒，齐一生物长期经营ELISA试剂盒（免费代测）及抗体、分子学生物试剂盒、金标法检测试剂盒、比色法检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。齐一生物销售：021－6034 8496；181214 53965；173021 04490

NG0530   旋毛虫抗原检测试剂盒                   血清         20T/40T                      

NG0531   旋毛虫抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0532   肺吸虫抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0533   肝吸虫抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0534   疟原虫抗体IgG\IgM检测试剂盒          血清         20T/40T                       【人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒厂家】

NG0536   真菌分离培养基试剂盒                            10T                    

NG0537   念珠菌显色分离培养基试剂盒                       18T                    

NG0538   皮肤癣菌鉴别培养基试剂盒                            20T                    

NG0539   霉菌滴虫培养基试剂盒                   分泌物     20T                    

NG0540   杜克雷嗜血杆菌选择性培养基试剂盒                            20T                    

NG0541   大肠菌群快速检测纸片                            60T/100T                   

NG0542   幽门螺杆菌快速检测试剂     脲酶法     胃粘膜/牙垢   50T/100T                   

NG0543   解脲支原体培养基试剂盒液体                       20T                    

NG0544   支原体固体培养基试剂盒                       20T                    

NG0545   解脲-人型支原体复合培养基试剂盒                      20T                    

NG0546   淋球菌培养管                   分泌物     20T                    

NG0547   淋球菌TM培养基试剂盒               分泌物     10T                    

NG0548   淋球菌鉴定药敏试剂              分泌物     20T                    

NG0549   解脲支原体选择性培养基试剂盒                   分泌物     20T                    

NG0550   解脲支原体药敏盒                   分泌物     20T                    

NG0551   肺炎支原体选择性培养基试剂盒                   分泌物     20T                     【人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒厂家】

NG0552   人型支原体选择性培养基试剂盒                   分泌物     20T                    

NG0553   人型支原体药敏盒                   分泌物     20T                    

NG0554   生殖支原体选择性培养基试剂盒                   分泌物     20T                    

NG0555   霉菌培养基试剂盒                   分泌物     20T                    

NG0556   自然沉降法液基细胞处理试剂盒         100人份/件    88元/人份                                  

NG0557   宫颈脱落细胞处理试剂盒     100人份/件    88元/人份                                  

NG0558   痰液处理试剂盒     100人份/件    88元/人份                                  

NG0559   穿刺、刷检细胞处理试剂盒         50人份/件       88元/人份                                  

NG0560   浆膜腔积液处理试剂盒         50人份/件       108元/人份                               

QKT0556  同型半胱氨酸/HCY酶法                  康特                                    

QKT0557  结核分枝杆菌抗体IgG检测试剂盒酶联免疫法                                              

QKT0558  丙型肝炎病毒核心抗原检测试剂盒                                                  

9233299  Toothbelt, X-drive, P3xx1                   CNY                             

9233276  Motor, bar-code camera, Y-motor           CNY                             

9233272  LabHand motor (Plato 1***)            CNY                              【人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒厂家】

9237711  #BR M48, Installation               CNY                             

9237164  Bezel XYP                   CNY                             

9237161  Adapter Vertical Height            CNY                             

9237121  Upgrade App., R.E.A.L. Prep 96                CNY                             

9237112  Upgrade App., QIAamp 96 Swab     Take advantage of our instrumentation ａnd molecular biology expertise to install QIAamp 96 DNA Swab on your BioRobot System.  The onsite protocol development visit includes all travel ａnd labor for the visit to install the standard QIAGEN QIAamp 96 DNA Swab protocol.  Also included is a QIAamp 96 DNA Swab BioRobot 9604 Kit (12-plates) SAP #965842    CNY                             

9237103  Upgrade, BR plus 3 ext. devices       Upgrade BioRobot with three approved integration devices CNY                             

9236355  Sample Needle, short                CNY                             

9232936  Microplate Reader (UV-340nm)Module           CNY                             

9232915  Washer Board           CNY                             

9236325  Sample Valve             CNY                             

9230821  Rack for reagents,Troughs(for 6 troughs)                  CNY                             

4823024  careHIV-1 RT-PCR ASSAY           CNY                             

4861014  careEBV PCR ASSAY I                 CNY                             

130823     NeXtal CubicPhase 2w Mo/C 8% µplate (2)               CNY                             

130822     NeXtal CubicPhase 1w Mo/C 8% µplate (10)            CNY                             

130723     NeXtal Tubes Classics II Suite            CNY                              【人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒厂家】

4513363  artus HI Virus-1 QS-RGQ Kit (24) CE                  CNY                             

4510003  artus Enterovirus LC RT-PCR Kit (24) RUO                 CNY                             

9011277  Plate, ground, auto vac, BR8000              CNY                             

330504     RT² SYBR Green qPCR  Mastermix (6)              CNY                             

9010975  BGR 4er-Incubator PK80          CNY                             

133293     NeXtalStock Zinc acetate (200)                 CNY                             

1014051  Ni-NTA Silica (10 g)            CNY                             

1013342  Ni-NTA HisSorb Plates, black (100)            CNY                             

1005485  Q-Solution,5 x (2ml/2)                CNY                             

997012     Accessory Trough (10)               CNY                             

995999     Starter Pack, M48             CNY                             

674791     ipsogen ABL Control Gene 4 Standards             CNY                             

tab-coj�2>�R��P�       CNY                             

 

9240377  QIAcube, Installation       The Installation, QIAcube facilitates the quick implementation of the QIACube into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the QIAcube instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.  CNY                             

9239571  BR Universal, Basic Agreement       The Basic Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9239570  BR Universal, Premium Agreement         The Premium Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).    CNY                             

9020032  FOOTSET, RGQ                   CNY                             

9019986  PCB, small breakout, 12 core, C12            CNY                             

9019479  UPPER BUFFER LID GS              CNY                             

9238864  BR M48, Premium Agreement         The Premium Cover Agreement M48 provides full service coverage for the BioRobot M48. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY                             

9238852  LiquiChip, Basic Agreement     The Basic Cover Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9241224  QIAxcel, Inspection Service      "Repair service delivery not applicable

Service response time (repair) not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: not included

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: Yes, included" CNY                             

9241197  Silicone sealant, RTV 108, QX           CNY                             

9241183  Sensor, Y, with cable, QX           CNY                             

9233329  Belt, tooth, X-drive, BR8000             CNY                             

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NG0473   19项冻干生化质控血清                  支                                

NG0474   解脲支原体抗体IgG\IgM检测试剂盒          血清         24T/48T                      

NG0475   沙眼衣原体抗原检测试剂盒                   分泌物     20T                    

NG0476   沙眼衣原体抗体IgG\IgM检测试剂盒          血清         24T/48T                      

NG0477   人型支原体抗体IgG\IgM检测试剂盒          血清         24T/48T                      

NG0478   生殖支原体抗体IgG\IgM检测试剂盒          血清         24T/48T                      

NG0479   淋球菌抗体IgG\IgM检测试剂盒          血清         24T/48T                      

NG0480   梅毒抗体IgG\IgM检测试剂盒               血清         24T/48T                      

NG0481   单纯疱疹病毒Ⅱ抗体IgG\IgM检测试剂盒          血清         24T/48T                      

NG0482   人类乳头瘤病毒抗体HPV检测试剂盒                  血清         24T/48T                      

NG0483   细菌性阴道病BV胺法           分泌物     20T                    

NG0484   梅毒特异性抗体试条检测试剂盒                   血清         1T                      

NG0485   淋球菌抗原检测试条检测试剂盒                   分泌物     1T                      

NG0486   RC-J013，RPR胶乳法              血清         120T                            【人碱性成纤维细胞生长因子9(bFGF-9)ELISA试剂盒厂家】

NG0487   TPPA梅毒确诊试剂胶乳法             分泌物     100T                           

NG0488   肺炎支原体抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0489   肺炎衣原体抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0490   结核抗体IgG\IgM检测试剂盒               血清         20T/40T                      

NG0491   幽门螺杆菌抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0492   呼吸道合胞病毒抗体IgG\IgM检测试剂盒          血清         20T/40T                      

NG0493   腺病毒抗体IgG\IgM\IgA检测试剂盒           血清         20T/40T                      

NG0494   EB病毒衣壳抗体IgG\IgM\IgA检测试剂盒          血清         20T/40T                      

NG0495   狂犬病毒抗体IgG\IgM检测试剂盒               血清         20T/40T                      

NG0496   狂犬病毒抗原检测试剂盒              血清         20T/40T                      

133220     NeXtalStock Sodium ac. H2O pH 5.0(200)                 CNY                              【人碱性成纤维细胞生长因子9(bFGF-9)ELISA试剂盒厂家】

301704     HiPerFect Transfection Reagent (0,5 ml)                   CNY                             

301427     Effectene Transfection Reagent (4x1ml) 4 x 1 ml Effectene Reagent, Enhancer, Buffer; for 160 transfections in 60 mm dishes or 640 transfections in 12-well plates      CNY                             

51326       QIAamp DNA Mini QIAcube Kit (240)                CNY                             

9012672  BGR Peripumpe PP-4 Frontbl.gerade (7135)            CNY                             

9002011  Rotor-Gene Q MDx 2Plex HRM no PC               CNY                             

292063     mericon Sheep Kit (24)              CNY                             

9001912  PyroMark Q24 MDx, PrioPLUS         "PyroMark Q24 MDx Instrument

Installation of PyroMark Q24  & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Annual Inspections (1 onsite PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)

(In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)"         CNY                             

9001910  QIAgility HEPA/UV (w/o PC) PrioPLUS      "QIAgility HEPA/UV (w/o PC)  Instrument

Installation of QIAgility instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

133116     NeXtalStock Sodium thiocyanate (200)             CNY                             

967142     RNeasy 96 BioRobot 9604 Kit (12)  For 12 x 96 total ａnd cytoplasmic RNA preps on the BioRobot 9604: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, Square-Well Blocks, RNase-free Reagents ａnd Buffers         CNY                             

962141     QIAprep 96 Turbo BioRobot Kit (4)  For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 ａnd QIAprep 96 Plates; Flat-Bottom Blocks ａnd Lids, Reagents, Buffers, Collection Microtubes (1.2 ml) ａnd Caps, 96-Well Microplates RB ａnd Lids, Tape Pads         CNY                             

960241     QIAGEN Plasmid Plus 96 BioRobot Kit (4)                  CNY                             

51304       QIAamp DNA Mini Kit (50)       For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)     CNY                             

978756     PyroMark CpG Assay 96 well (200)           CNY                             

9012712  Z-motor (Plato 7) left                 CNY                             

9012696  Set of X-flat band cable (Plato 7)               CNY                              【人碱性成纤维细胞生长因子9(bFGF-9)ELISA试剂盒厂家】

133239     NeXtalStock Sodium citrate pH 7.0 (200)                  CNY                             

133238     NeXtalStock Sodium citrate pH 5.8 (200)                  CNY                             

9002127  QIAsymphony RGQ, Priority    QIAsymphony RGQ Priority includes the QIAsymphony RGQ instruments, installations ａnd extended warranties. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

59203       EpiTect Whole Bisulfitome Kit (25)           CNY                             

59164       EpiTect Plus LyseAll Bisulfite Kit (48)                  CNY                             

929526     QX Alignment Marker 50 bp/1 kb (1.5 ml)                 CNY                             

133218     NeXtalStock MOPS pH 8.0 (200)                CNY                             

301605     RNAiFect Transfection Reagent (1ml)      RNAiFect Reagent ａnd buffer, for up to 170 transfections in 24-well plates; up to 500 transfections in 96-well plates  CNY                             

301525     TransMessenger Tranfect. Reagent(0.5ml)     For 60 transfections in 6-well plates or 80 transfections in 12-well plates         CNY                             

52304       QIAamp RNA Blood Mini Kit (50)     For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers    CNY                             

51306       QIAamp DNA Mini Kit (250)     For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)     CNY                             

9002012  Rotor-Gene Q MDx 2plex HRM Platform                  CNY                             

970530     NRAS Pyro Kit (24)            CNY                             

970022     PyroMark Q24 CpG MLH1 (4 x 24)   For quantification of methylation level in region -209 to -188 from the transcription start site. CNY