检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人中性粒细胞碱性磷酸酶(NAP)ELISA试剂盒厂家】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人中性粒细胞碱性磷酸酶(NAP)ELISA试剂盒厂家】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人中性粒细胞碱性磷酸酶(NAP)ELISA试剂盒厂家】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【人中性粒细胞碱性磷酸酶(NAP)ELISA试剂盒厂家】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

9015603  Upgrade, LiquiChip IS 2.x XYP, PLUS                   CNY                             

9015523  O-rings, plunger (12)                  CNY                             

9015522  O-rings, nozzles (6)           CNY                             

9019167  Linear actuator, PyroQ96MD           CNY                             

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9021255  Tool, thermistor calibration, RGx              CNY                             

9021233  Vacuum Pump (UK plug), PyroQ24VW              CNY                             

130927     NeXtal DWBlock JCSG Core IV Suite                   CNY                             

338176     RT² Optical Cap Strips (2 x 24)          CNY                             

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9001293  QIAcube (230V)        Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits, 1-year warranty on parts ａnd labor CNY                              【人中性粒细胞碱性磷酸酶(NAP)ELISA试剂盒厂家】

4866004  PG FMDV RT-PCR Kit (48) LE Z                   CNY                             

4867004  PG FMDV RT-PCR Kit (48) Z               CNY                             

4870004  PG Influenza A RT-PCR Kit (48) Z               CNY                             

130907     NeXtal DWBlock Anions Suite           CNY                             

4559263  artus C.trachomatisPlus RG PCR Kit(24)CE                CNY                             

4601203  artus Malaria RG PCR Kit (24) RUO          CNY                             

4843024  pg HPV PCR ASSAY            CNY                             

130826     NeXtal CubicPhase 3w Mo/C 8% µplate (10)            CNY                             

130727     NeXtal Tubes JCSG Core IV Suite                CNY                             

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129916     QIAGEN RNase Inhibitor (500 reactions)          "QIAGEN RNase Inhibitor is a protein of nonhuman origin that binds noncovalently to an inactivates a variety of ribonucleases (e.g., RNase A, B, ａnd C). It does not interfere with RNase T1,T2, H, U1, U2, or CL3. QIAGEN RNase Inhibitor is a polyclonal antibody which is highly active ａnd inhibits more than 90% of RNase activity, whereas HPRI (human placental RNase inhibitor) inhibits only 50%. For this reason, it can be used at lower concentrations compared to other RNase

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129115     Nuclease-Free Water (1000 ml)                CNY                             

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76163       RNAlater TissueProtect Tubes (20x5ml)  For stabilization of RNA in 20 x 500 mg tissue samples: 20 screw-top tubes containing 5 ml RNAlater RNA Stabilization Reagent each     CNY                             

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74136       RNeasy Plus Mini Kit (250)                 CNY                             

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QY-x2403 人肝细胞QSG-7701                                                      

QY-x2404 猪肾细胞系PK-15                                                

QY-x2405 猪髋动脉内皮细胞PIEC                                                       

QY-x2406 前列腺癌细胞系PC-3                                                  

QY-x2407 大鼠肾上腺嗜铬细胞瘤细胞(高分化)PC-12                                              

QY-x2408 大鼠肾上腺嗜铬细胞瘤细胞(未分化)PC-12                                              

QY-x2409 人胰腺癌细胞PANC-1                                                  

QY-x2410 逆转录病毒包被的NIH3T3细胞PA317                                                      

QY-x2411 小鼠骨髓瘤细胞P3/NSI/1-Ag-4【NS-1】                                                    

QY-x2412 鼠成纤维细胞系 NIH/3T3 询价 ATCC                                                    

QY-x2413 大鼠肾细胞 NRK 询价 ATCC                                                     

QY-x2414 人胃癌细胞 NCI-N87 [N87] 询价 ATCC                                                    

QY-x2415 人Burkitt＇s淋巴瘤细胞 NAMALWA 询价 ATCC                                                       

QY-x2416 人胚肺细胞 MRC-5 询价 ATCC                                                

QY-x2417 人胃癌细胞 MGC80-3 询价 ATCC                                                    

QY-x2418 犬肾细胞系 MDCK 询价 ATCC                                                  【人中性粒细胞碱性磷酸酶(NAP)ELISA试剂盒厂家】

QY-x2419 牛肾细胞 MDBK (NBL-1) 询价 ATCC                                                

QY-x2420 人乳腺癌细胞 MDA-MB-231 询价 ATCC                                                 

QY-x2421 人乳腺癌细胞 MCF7 询价 ATCC                                                       

QY-x2422 大鼠成肌细胞 L6 询价 ATCC                                                    

QY-x2423 小鼠成纤维细胞 L929 询价 ATCC                                                    

QY-x2424 人T淋巴细胞白血病细胞 Jurkat 询价 ATCC                                                  

QY-x2425 人结肠癌细胞 HT-29 询价 ATCC                                                      

QY-x2426 人纤维肉瘤细胞系 HT-1080 询价 ATCC                                                  

QY-x2427 人卵巢癌细胞 HO-8910                                                     

QY-x2428 人肝正常细胞 HL-7702 询价 ATCC                                                  

QY-x2429 人原髓细胞白血病细胞 HL-60 询价 ATCC                                                      

QY-x2430 肾小管株 HK-2 询价 ATCC                                                        

QY-x2431 小鼠肝癌细胞 Hepa 1-6 询价 ATCC                                                

QY-x2432 人肝癌细胞系 Hep-3B 询价 ATCC                                                   

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