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齐一生物现货供应人ELISA试剂盒，大鼠ELISA试剂盒，小鼠ELISA试剂盒等现货充足，购买任意ELISA试剂盒都享受免费代测服务，更多进口原装品牌试剂盒供应齐一生物销售：021－6034 8496；181214 53965；173021 04490

【人蛋白激酶B(PKB)ELISA试剂盒厂家】酶法检测试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器，EP 管及一次性吸头

3. 37℃恒温箱，双蒸水或去离子水

4. 吸水纸

试剂盒安全性提示：

1.工作人员实验中避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

【人蛋白激酶B(PKB)ELISA试剂盒厂家】操作注意事项：

1.试剂盒应按标签说明书储存，使用前恢复到室温30分钟后方可使用。稀稀过后的标准品应丢弃，不可保存。

2.实验中不用的板条应立即放回包装袋中，密封保存，以免变质。

3.不用的其它试剂应包装好或盖好。不同批号的试剂不要混用.请在试剂盒保质前使用。

4.使用一次性的吸头以免交叉污染，吸取终止液和底物A、B液时，避免使用带金属部分的加样器。

5.使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。

6.洗涤酶标板时应充分拍干，不要将吸水纸直接放入酶标反应孔中吸水。

7.底物A应挥发，避免长时间打开盖子。底物B对光敏感，避免长时间暴露于光下。避免用手接触，有毒。实验完成后应立即读取OD值。

8.加入试剂的顺序应一致，以保证所有反应板孔温育的时间一样。

9.按照说明书中标明的时间、加液的量及顺序进行温育操作。

【人蛋白激酶B(PKB)ELISA试剂盒厂家】齐一生物客户寄标本时需注明以下情况：

1、标本编号；2、所测项目；3、是否做复孔；3、联系方式；4、实验后标本是否寄回。

客户必知：

客户应对所提供的材料及信息负责，如因客户提供的材料及信息不准确而引起的实验延误或经济损失由客户承担。

齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

样本保存条件：

1.2-8℃冰箱多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【人蛋白激酶B(PKB)ELISA试剂盒厂家】齐一生物供应，以上资料供参考如有疑问需要详细资料请联系我们客服人员，齐一生物销售：021－6034 8496；181214 53965；173021 04490

301107     PolyFect Transfection Reagent(4 x1.0 ml)        "

For 100-260 transfections in 60 mm dishes or 200-400 transfections in 6-well plates." CNY                             

54104       QIAamp cador Pathogen Mini Kit (50)              CNY                             

9001946  QIAcube (220-240V) Promo              CNY                             

971061     therascreen® MGMT Pyro Kit (48)           CNY                             

293035     mericon Apricot kernels (96)             CNY                             

929520     QX Alignment Marker 15bp/500bp (1.5 ml)              CNY                             

9012643  Bottle, system liquid                  CNY                              【人蛋白激酶B(PKB)ELISA试剂盒厂家】

9012596  Disposable Tips, 300µl (960)    Conducting disposable tips       CNY                             

133121     NeXtalStock Lithium citrate  (200)                  CNY                             

133119     NeXtalStock Ammonium citrate  (200)           CNY                             

133190     NeXtalStock D-(+)-Glucose (200)               CNY                             

51162       QIAamp 96 DNA Blood Kit (12)        For 12 x 96 DNA preps: 12 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels       CNY                             

50112       QIAamp UCP PurePathogen Blood Kit (10)               CNY                             

133104     NeXtalStock Sodium acet.  (200)            CNY                             

9015468  Sensor, photo, PM-L24              CNY                             

9001871  EZ1 Advanced, System     EZ1 Advanced, System combines the EZ1 Advanced system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY                             

9018458  Screw, w finishing washer (4), QX             CNY                             

9018447  Purge Tool, cartridge, QX                  CNY                             

9012412  Incubator Tower (65°) for Plato 3 hold              CNY                             

9012392  Bottle, waste, 10 l, BR8000              CNY                             

205111     Omniscript RT Kit (50)      For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water         CNY                             

9018425  Door, sample, QX              CNY                             

9002123  QIAxcel Advanced System        QIAxcel Advanced System combines the QIAxcel Advanced instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.          CNY                              【人蛋白激酶B(PKB)ELISA试剂盒厂家】

57714       QIAamp MinElute Virus Vacuum Kit (50)         For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)      CNY                             

972012     PyroMark Q96 CpG p16 (96)    For quantification of methylation level in p16 CNY                             

133227     NeXtalStock Sodium cacodyl. pH 6.0 (100)                CNY                             

301108     PolyFect Transfection Reagent (100 ml)  For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.      CNY                             

56304       QIAamp DNA Micro Kit (50)    For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)     CNY                             

9002031  Rotor-Gene Q MDx 5Plex HRM no PC               CNY                             

9002018  Rotor-Gene Q MDx 2plex HRM, Priority Rotor-Gene Q 2plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

970902     PyroMark Q24 Advanced Kit (4x24)                   CNY                             

133211     NeXtalStock Magnesium chloride H2O (200)            CNY                             

133195     NeXtalStock Sodium tartrate pH 5.6 (200)                CNY                             

QKT0640  α-L-岩藻糖苷酶测定试剂盒AFUPNPF-两点法                                                 

QKT0641  α-L-岩藻糖苷酶测定试剂盒AFUCNPF-连续监测法                                                 

QKT0642  α-淀粉酶测定试剂盒AMY   EPS连续检测法                                           

QKT0643  血管紧张素化酶测定试剂盒ACE两点法                                                   【人蛋白激酶B(PKB)ELISA试剂盒厂家】

QKT0644  乳酸脱氢酶测定试剂盒LDH-L      速率法                                         

QKT0645  肌酸磷酸激酶测定试剂盒CK速率法                                                

QKT0646  肌酸激酶同工酶测定试剂盒CK-MB速率法                                                      

QKT0647  超敏C-反应蛋白测定试剂盒H-CRP免疫比浊法                                                       

QKT0648  α-羟基丁酸脱氢酶测定试剂盒α-HBDH速率法                                                      

QKT0649  D二聚体测定试剂盒D-Dimer免疫比浊法                                               

QKT0650  同型半胱氨酸测定试剂盒HCY酶循环法                                                  

QKT0651  二氧化碳测定试剂盒CO2     比色法                                         

QKT0652  镁测定试剂盒Mg甲基百里香酚兰法                                               

QKT0653  载脂蛋白测定试剂盒APoA1 免疫比浊法                                         

QKT0654  载脂蛋白B测定试剂盒ApoB        免疫比浊法                                         

QKT0655  高密度脂蛋白胆固醇测定试剂盒HDL-C      直接法                                         

QKT0656  总胆固醇测定试剂盒TC酶法                                                     

QKT0657  甘油三脂测定试剂盒TG酶法                                                    

QKT0658  低密度脂蛋白胆固醇测定试剂盒LDL-C直接法                                               

QKT0659  脂蛋白a测定试剂盒Lpa免疫比浊法                                                【人蛋白激酶B(PKB)ELISA试剂盒厂家】

QKT0660  胱抑素C测定试剂盒CysC     胶体金颗粒免疫比浊法                                              

QKT0661  N-乙酰-β-D-氨基葡萄糖苷酶测定试剂盒NAG两点法                                                    

QKT0662  β2-微球蛋白测定试剂盒β2-MG免疫比浊法                                                 

QKT0663  肌酐测定试剂盒CRE酶法                                                  

QKT0664  尿素测定试剂盒Urea谷氨酸脱氢酶法                                                     

QKT0665  血清果糖胺测定试剂盒FA    终点法                                         

QKT0666  血糖测定试剂盒GLU     氧化酶法                                              

QKT0667  免疫球蛋白M测定试剂盒lgM免疫比浊法                                                      

QKT0668  白蛋白测定试剂盒Alb溴甲酚绿法                                                    

QKT0669  总蛋白测定试剂盒TP双缩脲法                                                

QKT0670  血氨测定试剂盒AMM终点法