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齐一生物科技(上海)有限公司

产品介绍

人血管生成素2(ANG-2)ELISA试剂盒厂家

价 格:¥电议

型 号:

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生产地:其他访问量:18次

发布日期:2016/8/17 18:40:25

更新日期:2016/8/17 18:40:25

详细内容

 人血管生成素2(ANG-2)ELISA试剂盒厂家科研之路。您我并肩同行!齐一生物具有完善的ELISA试剂盒开发平台,成熟的抗原、抗体研发系统,熟练掌握各种酶联技术,如双抗夹心法、(直接)竞争法、间接竞争法、阻断法、间接法、双抗原夹心法等方法,结合我公司的诊断试剂开发团队我们可以有效的将试剂盒开发为临床诊断级别,质量处于全国前茅。齐一生物针对中高端客户推出自主品牌QIYBO试剂盒为了保证客户用货质量,我公司采用纯进口原料现包被生产,质量稳定高. 齐一生物销售:0216034 8496181214 53965173021 04490欢迎您的咨询购买

【友情提示】:本产品仅供科研研究使用,不得用于人体临床直接检测。避免给您带来不必要的损失,请仔细阅读购买说明!

齐一生物现货供应人ELISA试剂盒,大鼠ELISA试剂盒,小鼠ELISA试剂盒等现货充足,购买任意ELISA试剂盒都享受免费代测服务,更多进口原装品牌试剂盒供应齐一生物销售:021-6034 8496;181214 53965;173021 04490

人血管生成素2(ANG-2)ELISA试剂盒厂家酶法检测试验所需自备物品:

1. 酶标仪(450nm 波长滤光片)

2. 高精度移液器,EP 管及一次性吸头

3. 37℃恒温箱,双蒸水或去离子水

4. 吸水纸

试剂盒安全性提示:

1.工作人员实验中避免直接接触终止液和底物AB。一旦接触到这些液体,请尽快用水冲洗。

2.实验中不要吃喝、抽烟或使用化妆品。

3.不要用嘴吸取试剂盒里的任何成份。

人血管生成素2(ANG-2)ELISA试剂盒厂家操作注意事项:

1.试剂盒应按标签说明书储存,使用前恢复到室温30分钟后方可使用。稀稀过后的标准品应丢弃,不可保存。

2.实验中不用的板条应立即放回包装袋中,密封保存,以免变质。

3.不用的其它试剂应包装好或盖好。不同批号的试剂不要混用.请在试剂盒保质前使用。

4.使用一次性的吸头以免交叉污染,吸取终止液和底物AB液时,避免使用带金属部分的加样器。

5.使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。

6.洗涤酶标板时应充分拍干,不要将吸水纸直接放入酶标反应孔中吸水。

7.底物A应挥发,避免长时间打开盖子。底物B对光敏感,避免长时间暴露于光下。避免用手接触,有毒。实验完成后应立即读取OD值。

8.加入试剂的顺序应一致,以保证所有反应板孔温育的时间一样。

9.按照说明书中标明的时间、加液的量及顺序进行温育操作。

人血管生成素2(ANG-2)ELISA试剂盒厂家齐一生物客户寄标本时需注明以下情况:

1、标本编号;2、所测项目;3、是否做复孔;3、联系方式;4、实验后标本是否寄回。

客户必知:

客户应对所提供的材料及信息负责,如因客户提供的材料及信息不准确而引起的实验延误或经济损失由客户承担。

齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性,在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

样本保存条件:

1.2-8℃冰箱多放2

2.-20-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱,可以放一年

样本收集

1.血清样本用干燥管(红色)或者促凝管(黄色)收集

2.血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)

3.动物组织匀浆(细胞):

              1g组织加入9ml PBS(浓度为0.1MpH7.4的)测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本:1g样本+9ml PBS(浓度为0.01MpH7.4的)

没有PBS的话也可以用一下生理盐水,还是用PBS

样本离心转速及时间

1.试管采集的样本:3000转,离心五分钟取上清

2.离心管或者EP管采集的样本:5000--80003分钟取上清

3..样本保存--如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

人血管生成素2(ANG-2)ELISA试剂盒厂家齐一生物供应,以上资料供参考如有疑问需要详细资料请联系我们客服人员,齐一生物销售:0216034 8496181214 53965173021 04490

9239125  Cable ethernet crossover,  Kloehn                   CNY                             

9239089  User Manual, BioSprint 15                CNY                             

9232687  Hood f. Tip Disposal Station 2.01              CNY                             

9232627  Bottle, 500-ml, white luer adapter          CNY                             

9232558  Vacuum manifold, blanks, 6S            CNY                             

9238976  Tip and Sample Rack, V2          CNY                             

9238787  Angle rotor 30 x 1.5 / 2.2 ml             CNY                             

9020318  PM kit, C18 & QIAxtractor, CX1                 CNY                             

9020317  PM kit, C18 & QIAxtractor (HEPA), CX1            CNY                              【人血管生成素2(ANG-2)ELISA试剂盒厂家】

9017245  Microplate Station 8000, seesaw             CNY                             

9020226  Belt, X-axis assembly, C4x                  CNY                             

9020211  Switch, assembly, lid, Nov08-Jun09, C1x           CNY                             

9020210  Foot assembly, QGX          CNY                             

9020154  USB Connector&Cable; RGQ             CNY                             

9020091  Hall Sensor Board, TLyser 2               CNY                             

9239514  EX SD Controller                CNY                            

9239512  EX XY, Platter Interconnect               CNY                             

9239509  EX XY, Controller Board XYP              CNY                             

9236529  Tubing Guide, Probe Holder, BR8 RD                CNY                             

9236512  #BR 9604, PM Service      The Overhaul covers all travel and labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked and inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.     CNY                             

9232778  Card, x-main, PCON, 2               CNY                             

9239314  Reservoir, OLF, with sensor, RapidPlate            CNY                             

9236449  Travel Charge, zone D               CNY                             

9236434  Sensor, rotary home, TWI                  CNY                             

9239174  Cooling Block , 24-tube, 1.5 ml, tall                   CNY                              【人血管生成素2(ANG-2)ELISA试剂盒厂家】

9232634  Bottle, 250-ml, blue fitting                CNY                             

9017157  BR MDx CTM 48 PCR Setup, PRO              CNY                             

9232475  Cover, SIS, BR9604, V1.01                 CNY                             

9232334  Cable, magnet valve, DISU, BR8000                 CNY                             

9232331  Cable, power, dilutor 5-8, BR8000            CNY                             

9238779  Agreement, MDx, 2PM, 1yr    An agreement for preventive maintenance provides coverage for travel, labor, and parts, plus the cost of consumables required for two maintenance visits during 1 year. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives.          CNY                             

9238773  Project Pack     Includes 12 working days (on-site or off-site work) by a QIAGEN Instrumentation Service Specialist, valid for 3 years.  These days can be used for writing and/or optimization of protocols or for training. It includes all travel and labor charges. Parts, consumable chemistries, and accessories are not included. Custom protocols are the sole responsibility of the customer and neither warrantied nor guaranteed.       CNY                             

9238911  Power supply 40W, BS96          CNY                             

QY-L0346 1,5-O-二咖啡酰奎宁酸30964-13-7        Cynarin     30964-13-7       20mg                          

QY-L0347 1,3-O-二咖啡酰奎宁酸1182-34-9 Cynarin     1182-34-9         20mg                           【人血管生成素2(ANG-2)ELISA试剂盒厂家】

QY-L0348 绿原酸327-97-9      Chlorogenic acid       327-97-9  20mg                          

QY-L0349 新绿原酸906-33-2 Neochlorogenic acid         906-33-2  20mg                          

QY-L0350 隐绿原酸905-99-7 Cryptochlorogenic acid    905-99-7  20mg                          

QY-L0351 异绿原酸A2450-53-5     Isochlorogenic acid A       2450-53-5         20mg                          

QY-L0352 异绿原酸B14534-61-3   Isochlorogenic acid B       14534-61-3       20mg                          

QY-L0353 异绿原酸C32451-88-0   Isochlorogenic acid C        32451-88-0       20mg                         

QY-L0354 冬绿苷490-67-5      Gaultherin        490-67-5  20mg                          

QY-L0355 甜叶悬钩子苷64849-39-4    Rubusoside       64849-39-4     20mg                          

QY-L0356 乙氧基白屈菜红碱152743-19-6   Ethoxychelerythrine         152743-19-6    20mg                          

QY-L0357 紫萁酮     Osmundacetone                20mg                          

QY-L0358 二氢姜黄素     Dihydrocurcumin               20mg                          

QY-L0359 姜黄素458-37-7      Curcumin         458-37-7  20mg                          

QY-L0360 去甲氧基姜黄素22608-11-3 Demethoxycurcumin        22608-11-3       20mg                          

QY-L0361 双去氧基姜黄素24939-16-0 Bisdemethoxycurcumin   24939-16-0       20mg                          

QY-L0362 二苯乙烯苷82373-94-2 2,3,5,4-tetrahydroxyl diphenylethylene-2-o-glucoside    82373-94-2       20mg                          

QY-L0363 3,29-二苯甲酰基栝楼仁三醇873001-54-8   3,29-Dibenzoyl rarounitriol      873001-54-8    20mg                           【人血管生成素2(ANG-2)ELISA试剂盒厂家】

QY-L0364 β,β’-二甲基丙烯酰阿卡宁34539-65-6         β, β-dimethyl-acry-lalkannin       34539-65-6       20mg                          

QY-L0365 紫草素517-89-5      Shikonin   517-89-5  20mg                          

QY-L0366 紫草氰苷63492-69-3      Lithosprmoside         63492-69-3       20mg                          

QY-L0367 二氢辣椒素19408-84-5 Dihydrocapsaicin      19408-84-5       20mg                          

QY-L0368 辣椒素2444-46-4   Capsaicin         2444-46-4         20mg                          

QY-L0369 天然辣椒素404-86-4      Capsaicinoids   404-86-4  20mg                          

QY-L0370 槲皮素6151-25-3 117-39-5    Quercetin        6151-25-3 117-39-5 20mg                          

QY-L0371 二氢槲皮素480-18-2      Taxifolin   480-18-2  20mg                          

QY-L0372 槲皮苷522-12-3      Quercitrin         522-12-3  20mg                          

QY-L0373 异槲皮苷21637-25-2      Isoquercitrin    21637-25-2       20mg                          

QY-L0374 槲皮素-7-O-葡萄糖苷491-50-9      Quercetin-7-O-β-D-glucopyranoside      491-50-9  20mg                          

QY-L0375 槲皮素-3-O-β-D-葡糖糖-7-O-β-D-龙胆双糖苷   Quercetin-3-O-β-D-glucopyranosyl-7-O-β-D-gentiobiosid             20mg                           

QY-L0376 杨梅素529-44-2      Myricetin 529-44-2  20mg                          

-�l:�����o-ascii-font-family:Calibri;阴性对照A

 

  一般规定抑制率≥50%为阳性,<50%为阴性。

 

 

 定量测定

  ELSIA操作步骤复杂,影响反应因素较多,特别是固相载体的包被难达到各个体之间的一致,因此在定量测定中,每批测试均须用一系列不同浓度的参考标准品在相同的条件下制作标准曲线。测定大分子量物质的夹心法ELISA,标准曲线的范围一般较宽,曲线点的吸光度可接近2.0,绘制时常用半对数纸,以检测物的浓度为横坐标,以吸光度为纵坐标,将各浓度的值逐点连接,所得曲线一般呈S形,其头、尾部曲线趋于平坦,中央较呈直线的部分是理想的检测区域。

  测定小分子量物质常用竞争法,其标准曲线中吸光度与受检物质的浓度呈负相关。标准曲线的形状因试剂盒所用模式的差别而略有不同。ELISA测定的标准曲线注意图中横坐标为对数关系,这更有利于测定系统的表达。

        讲述完了以上ELISA实验的操作要点,可以发现其实很多步骤都并不复杂,重要的是操作的时候一定要注重细节,熟能生巧,才可以掌握ELISA实验的精髓所在。

9020713  Rail guide, x axis, C34                CNY                             

9020712  Rail, x axis, C34                  CNY                             

9020679  Rail, left, CX1            CNY                             

9020642  Waste chute, straight, SP, Cabinet Qsym                   CNY                             

9020637  Shelf, consumables, Cabinet Qsym            CNY                             

9020601  Shipping Box - DML2000           CNY                             

9020488  PCB, set, GSC            CNY                             

9020484  Pulley, GSC                 CNY                             

9017596  Controller, temp.TTM-004(SN274or higher)             CNY                             

9017594  BGR Tube Holder 5 & 6 ml                 CNY                              【人血管生成素受体/含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶1(Tie1)ELISA试剂盒现货】

9020257  Kit, screws, Qsym              CNY                             

9020245  QIAsymphony Cabinet AS                   CNY                             

9017344  CD User Manual LiquiChip 200                   CNY                             

9020240  PM kit, C18 & QIAxtractor (UV), CX1                CNY                             

9020219  Cable, HEPA filter, QGX             CNY                             

9020192  PCB, vac pump (incl. cable), C18               CNY                             

9239542  Assy, XY, Reflective Sensor                 CNY                             

9239528  Ferrule,Flangeless,BlueETFE,1/16tub(10)                  CNY                             

9020184  Gasket, centrifuge lid, QIAC              CNY                             

9020157  Bearing, rotor shaft, RG3                  CNY                             

9020153  8 Way Cable for LED Assy, RGQ                 CNY                             

9020147  Rotor-Gene ScreenClust HRM Software           CNY                             

9020134  Vacuum Bottle and cap, PyroVW              CNY                             

9020095  Right Ratchet Holder, TLyser 2                  CNY                             

9020087  Packaging, TLyser              CNY                             

9020082  Left Ratchet Holder, TLyser               CNY                              【人血管生成素受体/含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶1(Tie1)ELISA试剂盒现货】

9239511  XY, Platter Interconnect           CNY                             

9239347  Spotlight bulb (2), BR M96                CNY                             

9236494  LiquiChip, Full Agreement        The Full Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days). CNY                             

9232727  Block, 4-way, v1.02           CNY                             

9236437  Service, in-house, 1 day            CNY                             

9236428  Sensor, vertical home, TWI               CNY                             

9239150  Support, pod fixation (to base), TWII                CNY                             

QY-L0315 滇乌头70578-24-4 YunaconitineGuayewuanine B     70578-24-4       20mg                           【人血管生成素受体/含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶1(Tie1)ELISA试剂盒现货】

QY-L0316 (-)-丁香树脂酚-4-O-β-D-呋喃芹糖基-(12)-β-D-吡喃葡萄糖苷136997-64-3         (-)-Syringaresnol-4-O-β-D-apiofuranosyl-(12)-β-D-glucopyranoside       136997-64-3    20mg                          

QY-L0317 丁香树脂醇双葡萄糖苷         Syringaresinol-di-O-glucoside            20mg                          

QY-L0318 断血流皂苷A 916347-31-4    Clinodiside A     916347-31-4    20mg                          

QY-L0319          Cannabisin A              20mg                          

QY-L0320 杜鹃素24211-30-1 Farrerol    24211-30-1       20mg                          

QY-L0321 正丁基苯酞6066-49-5   3-n-Butylphathlide   6066-49-5         20mg                          

QY-L0322 丁烯基苯酞551-08-6      n-Butylidenephthalide     551-08-6  20mg                          

QY-L0323 洋川芎内酯A62006-39-7        Senkyunolide A         62006-39-7       20mg                          

QY-L0324 藁本内酯4431-01-0        Ligustilide         4431-01-0         20mg                          

QY-L0325 大蒜素(UV98%)539-86-6      Allicin        539-86-6  20mg                          

QY-L0326 蒜氨酸556-27-4      (S)-3-(Allylsulphinyl)-L-alanine  556-27-4  20mg                          

QY-L0327 大戟二烯醇514-47-6      Euphol      514-47-6  20mg                          

QY-L0328 灯盏花甲素    apigenin-7-O-glucronide                20mg                          

QY-L0329 野黄芩苷27740-01-8      Scutellarin        27740-01-8       20mg                          

QY-L0330 高黄芩素529-53-3 Scutellartln       529-53-3  20mg                          

QY-L0331 德尔塔林6836-11-9      Deltaline  1803152  20mg                           【人血管生成素受体/含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶1(Tie1)ELISA试剂盒现货】

QY-L0332 麦冬皂苷A 11054-24-3  Ophiopogonin A        11054-24-3    20mg                          

QY-L0333 麦冬皂苷B 38971-41-4  Ophiopogonin B        38971-41-4    20mg                          

QY-L0334 麦冬皂苷C911819-08-4         Ophiopogonin C       911819-08-4   20mg                          

QY-L0335 麦冬皂苷D945619-74-9          Ophiopogonin D       945619-74-9    20mg                          

QY-L0336 短葶山麦冬皂苷C87480-46-4       Liriope muscari baily saponins C       87480-46-4     20mg                          

QY-L0337 山麦冬皂苷B Liriopesides B            20mg                          

QY-L0338 鲁斯可皂苷元472-11-7 Ruscogenin       472-11-7  20mg                          

QY-L0339 多舌飞蓬苷     6-O-Caffeoylerigeroside                 20mg                          

QY-L0340 20830-75-5       Digoxin     20830-75-5       20mg                          

QY-L0341 莪术醇4871-97-0   Curcumol 4871-97-0         20mg                          

QY-L0342 莪术烯   Curzerene                20mg                          

QY-L0343 莪术二酮13657-68-6      Germacr-1(10)-ene-5,8-dione 13657-68-6       20mg                          

QY-L0344 18-二羟基蒽醌117-10-2    1,8-Dihydroxyanthraquinone  117-10-2  20mg                          

QY-L0345 3-O-咖啡酰奎宁酸(97%327-97-9           3-O-caffeoylquinic acid   327-97-9         20mg                           

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