检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【人α1β糖蛋白(α1β-GP)ELISA试剂盒现货】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

【人α1β糖蛋白(α1β-GP)ELISA试剂盒现货】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

【人α1β糖蛋白(α1β-GP)ELISA试剂盒现货】注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

【人α1β糖蛋白(α1β-GP)ELISA试剂盒现货】计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

9021101  Skin Panel, Lower Left Rear, DCU             CNY                             

9238279  PAXgene 96 Incubator Block (farbl.elox)          CNY                              【人α1β糖蛋白(α1β-GP)ELISA试剂盒现货】

9240677  Bumper, Round, Latch Lock, AP98            CNY                             

9240635  Filter, EMI,Modular, RJ45, AP196/98                 CNY                             

9240615  Fuse, 1.0A, FNM, AP96             CNY                             

9240612  Turk Sensor, Waste Bottle Level, AP                  CNY                             

9240603  Deck/Waste Overflow Sensor, AP196/98           CNY                             

9240583  Regulator, Pressure, Main, AP                   CNY                             

9240545  Hatch pad, Eppendorf, AP196/98               CNY                             

9240542  Lower Header, Capper Head, AP196                 CNY                             

9240521  Motor Assy, Shaker, AP98                  CNY                             

9240520  Motor Assy, Sz. 17, Common, AP98                   CNY                             

9240102  Re-Installation, LiquiChip          The Re-Installation, LiquiChip facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9019940  Power supply, vac pump, CX1           CNY                             

9019477  LASER MODUAL GS          CNY                             

9019476  Spacer, 0.2 mm short set, GSC                   CNY                             

9238888  PCB, Main-01, BS96                   CNY                             

9238886  Cable, HM, BS96               CNY                              【人α1β糖蛋白(α1β-GP)ELISA试剂盒现货】

9238854  #BR M96, Basic Agreement    The Basic Cover Agreement M96 provides full service coverage for the BioRobot M96. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238842  RS232 box, non-online fill, RapidPlate               CNY                             

9241215  Full Agreement Depot Repair, Shaker, 1yr       "The Full Agreement Depot Repair, Shaker provides full service coverage for the plate shaker used in the digene HPV application. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9238833  #BR 9604, Basic Agreement    The Basic Cover Agreement 9604, 1 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9238832  BR 9600, Basic Agreement      The Basic Cover Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238819  Rect. carrier for 5 glass tubes 25ml                   CNY                             

9237527  Software LiquiChip Workstation IS V2.2.          CNY                             

9237380  Installation, BR MDx & 2 react. setup     Installation ａnd training, including two customized reaction setup protocols         CNY                             

9237379  Installation, BR MDx & 1 react. setup     Installation ａnd training, including one customized reaction setup protocol CNY                             

9237378  BR MDx, Installation        The Installation, BR MDx facilitates the quick implementation of the BioRobot MDx into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot MDx instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9237175  Guard Fan, Platic XYP                CNY                             

9233209  Tubing, 3/2,                CNY                             

9233155  Power Cord, European              CNY                             

9236418  Board, CPU, TWI               CNY                             

9236345  Red LSR             CNY                             

rhIGF-I      重组人IGF-I    "Recombinant Human IGF-I

"       20µg                                    

rHuDES1-3/IGF1       重组人 DES1-3/ 胰岛样生长因子 -1   "Recombinant Human DES1-3/ Insulin-Like Growth factor 1

"       20μg                                    

rhIGF-BP3         重组人 IGF-BP3      "Recombinant Human IGF-BP3

"       5µg                             

rhLR3IGF-1       重组人长R3胰岛样生长因子-1   "Recombinant Human Long R3 Insulin-like Growth factor-1

"       20µg                                    

rhEGF       重组人表皮生长因子     "Recombinant Human Epidermal Growth Factor

"       100µg                                  

rhVEGF165       重组人血管内皮生长因子165      "Recombinant Human VEGF165

"       2µg                             

rhNT-4      重组人神经营养因子-4 "Recombinant Human Neurotrophin-4

"       2µg                             

rhCNTF     重组人睫状神经营养因子     "Recombinant Human Ciliary Neurotrophic Factor

"       5µg                             

rhNRG-1  重组人 NRG-1（表皮生长因子样结构域）         "Recombinant Human NRG-1(EGF-like domain)

"       10µg                                     【人α1β糖蛋白(α1β-GP)ELISA试剂盒现货】

rhErbB3-f 重组人ErbB3片段 "Recombinant Human ErbB3 Fragment

"       5µg                             

rhBTC       重组人乙胞素         "Recombinant Human Betacellulin

"       5µg                             

rhBMP-2  重组人骨形成蛋白-2      "Recombinant Human Bone Morphogenetic Protein-2

"       2µg                             

rhBMP-4  重组人骨形成蛋白-4      "Recombinant Human Bone Morphogenetic Protein-4

"       2µg                             

rhBMP-7  重组人骨形成蛋白-7      "Recombinant Human Bone Morphogenetic Protein-7

"       2µg                             

rhOPG-Fc 重组人骨保护素Fc融合蛋白        "Recombinant Human Osteoprotegerin/Fc Chimera

"       10µg                                    

rhNOGGIN        重组人NOGGIN蛋白     "Recombinant Human NOGGIN

"       5µg                             

rhGH         重组人生长激素     "Recombinant Human Growth Hormone

"       20µg                                    

rmGM-CSF        重组鼠粒细胞-巨噬细胞集落刺激因子        "Recombinant Murine Granulocyte- Macrophage Colony Stimulating Factor

"       5µg                             

rmTNF-α         重组鼠肿瘤坏死因子-α        "Recombinant Murine Tumor Necrosis Factor-alpha

"       5µg                             

rmLIF        重组鼠白血病抑制因子         "Recombinant Mouse Leukemia inhibitory factor

"       5µg                             

rmbFGF    重组鼠成纤维细胞生长因子-碱性        "Recombinant Murine Fibroblast Growth Factor-basic

"       10µg                                    

rmFGF-7/KGF    重组鼠成纤维细胞生长因子-7      "Recombinant Mouse Fibroblast Growth Factor-7

"       2µg                             

rmEGF      重组鼠表皮细胞生长因子     "Recombinant Murine Epidermal Growth Factor

"       100µg                                  

rmVEGF120      重组鼠血管内皮生长因子120      "Recombinant Murine Vascular Endothelial Growth Factor 120

"       2µg                             

rmVEGF165      重组鼠血管内皮生长因子165      "Recombinant Murine Vascular Endothelial Growth Factor 165

"       2µg                              【人α1β糖蛋白(α1β-GP)ELISA试剂盒现货】

rmNOGGIN      重组鼠NOGGIN      "Recombinant Murine NOGGIN

"       5µg                             

rmCXCL16         重组鼠CXCL16         "Recombinant Murine CXCL16

"       5μg                             

rrGM-CSF 重组鼠粒细胞-巨噬细胞集落刺激因子        "Recombinant Rat Granulocyte- Macrophage Colony Stimulating Factor

"       5µg                             

rrTNF-α 重组鼠肿瘤坏死因子-α        "Recombinant Rat Tumor Necrosis Factor-alpha

"       5µg                             

rr bFGF     重组鼠成纤维细胞生长因子-碱性        "Recombinant Rat Fibroblast Growth Factor-basic

"       10µg                                    

rrIGF         重组鼠IGF-I    "Recombinant Rat IGF-I

"       10µg