价格详细资料：试剂盒价格电议，齐一生物销售：021－6034 8496；181214 53965；173021 04490,齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大研究所.中科院科研单位一致肯定和认同。齐一生物大品牌保证，倾力为国内外科研院校实验室提供优质产品。

【人肌抑素(MSTN)ELISA试剂盒厂家】注意事项

1. 当混合蛋白溶液时应尽量轻缓，避免起泡。

2. 洗涤过程非常重要，不充分的洗涤易造成假阳性。

3. 一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。

4. 请每次测定的同时做标准曲线，做复孔。

5. 如标本中待测物质含量过高，请先稀释后再测定，计算时请后乘以稀释倍数。

6. 在配制标准品、检测溶液工作液时，请以相应的稀释液配制，不能混淆。

7. 底物请避光保存。

8. 不要用其它生产厂家的试剂替换试剂盒中的试剂。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人肌抑素(MSTN)ELISA试剂盒厂家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人肌抑素(MSTN)ELISA试剂盒厂家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人肌抑素(MSTN)ELISA试剂盒厂家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9236166       Safety shield, RapidPlate            CNY                           【人肌抑素(MSTN)ELISA试剂盒厂家】

9230863       Anpressplatte Rose-80              CNY                          

9235538       Teaching Tool              CNY                          

9230282       Cable, RS323, robot to syringe pump, 225            CNY                          

5050-2002    hc2 Software V2 Package         CNY                          

BS96RNA    BioSprint 96 to RNA (384) - UDP          CNY                          

9240730       Tubing, 1/4" OD, Red, AP         CNY                          

9240713       Poly Muffler Kit, AP96/196              CNY                          

9240690       Bearings, Lead Screws, AP              CNY                          

9243654       Comprehen Valid., Other Quant. Sys.      """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """ CNY                          

6000-5013    LumiCheck Plate User Package        CNY                          

288103  bactotype Mycoplasma Mg/Ms PCR Kit (24)        CNY                          

1048147       QIAamp Viral RNA Mini Accessory Set         CNY                          

270043  cattletype BHV1 gB Ab (5)              CNY                          

1019992       Buffer Set (RNeasy 96 BioRobot 9604 Kit            CNY                          

273401  pigtype Toxoplasma Ab (1)        CNY                          

273505  pigtype Trichinella Ab (20)         CNY                           【人肌抑素(MSTN)ELISA试剂盒厂家】

9019538       ZLVBelting, flat cogged, 6T, 2.5mm, CASx           CNY                          

9020591       Tray Assembly, DML 3000        CNY                          

9021007       Motor, Pipettor X, DCU            CNY                          

9013373       Fix kit, liquid handling, BR8000        CNY                          

9013315       Motor, handler T/L, BR3000            CNY                          

9013262       Cable, 24 V, power to power, 1200 mm         CNY                          

9013249       BGR Wash System             CNY                          

9013207       Service kit, accessories, BR8000             CNY                          

9013194       Tubing, L0 outlet, auto vac,BR8000 v1.00             CNY                          

9013187       Holder, retractable tubing, dispenser, B           CNY                          

800328  Miniprep, PCR purification (Easy-Read)         CNY                          

132109  EasyXtal 15-Well DG-Tool X-Seal (100)       "100 empty 15-well crystallization plates with dropguard, X-Seal crystallization supports

For manual setup of hanging ｄrop crystallization experiments."     CNY                          

132101  NeXtal DWBlock Adhesive Foils             CNY                          

132045  NeXtal Evolution µplate (10)             CNY                          

338172  RT2 Calibration Plate A             CNY                          

338125  384EZload Covers (4)        CNY                           【人肌抑素(MSTN)ELISA试剂盒厂家】

rbEGF    重组鼠表皮细胞生长因子     "Recombinant Rat Epidermal Growth Factor

"      20µg                           

rbCXCL16   重组牛碱性成纤维细胞生长因子       "Recombinant Bovine Basic Fibroblast Growth Factor

"      10µg                           

rProteinA      重组蛋白A "Recombinant Protein A

"      5mg                     

rProteinG      重组蛋白G "Recombinant Protein G

"      5mg                     

rCysProteinG       重组半胱氨酸-蛋白G     "Recombinant Cys-Protein G

"      5mg                     

rProteinA/G   重组蛋白A/G     "Recombinant Protein A/G

"      5mg                     

rStreptavidin  重组链霉亲和素       "Recombinant Streptavidin

"      1mg                     

rHSA     重组人血清白蛋白   "Recombinant Human Serum Albumin

"      1g                       

ECGS    内皮细胞生长因子 Endothelial Cell Growth Supplement 5 ml                     

SMCGS 平滑肌细胞生长因子      Smooth Muscle Cell Growth Supplement 5 ml                      【人肌抑素(MSTN)ELISA试剂盒厂家】

SMCGS-sf    平滑肌细胞生长因子（不含血清）   Smooth Muscle Cell Growth Supplement 5 ml                     

PGS       周细胞生长因子       Pericyte Growth Supplement      5 ml                     

MenCGS      脑膜细胞生长因子 Meningeal Cell Growth Supplement  5 ml                     

NPrCGS       神经前体细胞生长因子  Neural Precursor Cell Growth Supplement      5 ml                     

NGS      神经元生长因子     Neuronal Growth Supplement    5 ml                     

NPCDS 神经前体细胞分化生长因子 Neural Precursor Cell Differentiation Supplement   5 ml                     

OPCGS 少突胶质前体细胞生长因子      Oligodendrocyte Precursor Cell Growth Supplement     5 ml                     

OGS      少突胶质细胞生长因子       Oligodendrocyte Growth Supplement       5 ml                     

OPCDS 少突胶质前体分化生长因子      Oligodendrocyte Precursor Cell Differentiation Supplement  5 ml                     

SCGS    雪旺细胞生长因子 Schwann Cell Growth Supplement    5 ml                     

AGS      星形细胞生长因子 Astrocyte Growth Supplement   5 ml                     

AGS-a   星形细胞生长因子-动物      Astrocyte Growth Supplement-Animal     5 ml                     

MGS      小胶质细胞生长因子      Microglia Growth Supplement    5 ml                     

MGS      巨噬细胞生长因子   Macrophage Growth Supplement      50 ml                          

KGS      角质细胞生长因子 Keratinocyte Growth Supplement     5 ml                     

KGS-2   角质细胞生长因子（不含 BPE)       BPE-free Keratinocyte Growth Supplement    5 ml                      【人肌抑素(MSTN)ELISA试剂盒厂家】

KGS-acf       角质细胞生长因子（不含动物成分）    Keratinocyte Growth Supplement-animal component free     5 ml                        

MelGS   黑色素细胞生长因子    Melanocyte Growth Supplement       5 ml                     

MelGS-2       黑色素细胞生长因子（不含TPA)   TPA-free Melanocyte Growth Supplement      5 ml                     

FGS       成纤维细胞生长因子      Fibroblast Growth Supplement   5 ml                     

FGS-sf   成纤维细胞生长因子-sf  Fibroblast Growth Supplement-sf      5 ml