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人αN已酰氨基葡糖苷酶(αNAG)ELISA试剂盒现货

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发布日期:2016/8/17 22:34:23

更新日期:2016/8/17 22:34:23

详细内容

 人αN已酰氨基葡糖苷酶(αNAG)ELISA试剂盒现货规格:48T/96T

【友情提示】:本产品仅供科研研究使用,不得用于人体临床直接检测。避免给您带来不必要的损失,请仔细阅读购买说明!

本试剂盒仅供研究使用,齐一生物销售:0216034 8496181214 53965173021 04490欢迎选购!

操作步骤

1.准备试剂,样品和标准品

2.加入准备好的样品和标准品,37℃反应30分钟

3.洗板5次,加入酶标试剂,37℃反应30分钟

4.洗板5次,加入显色液AB37℃显色15分钟

5.加入终止液

6.15分钟之内读OD

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性,在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

人αN已酰氨基葡糖苷酶(αNAG)ELISA试剂盒现货样本保存条件:

1.2-8℃冰箱多放2

2.-20-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱,可以放一年

人αN已酰氨基葡糖苷酶(αNAG)ELISA试剂盒现货样本收集

1.血清样本用干燥管(红色)或者促凝管(黄色)收集

2.血浆用抗凝管,一般有EDTA抗凝(紫色)、枸橼酸钠抗凝(黑色或者蓝色)

3.动物组织匀浆(细胞):

              1g组织加入9ml PBS(浓度为0.1MpH7.4的)测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本:1g样本+9ml PBS(浓度为0.01MpH7.4的)

没有PBS的话也可以用一下生理盐水,还是用PBS

人αN已酰氨基葡糖苷酶(αNAG)ELISA试剂盒现货样本离心转速及时间

1.试管采集的样本:3000转,离心五分钟取上清

2.离心管或者EP管采集的样本:5000--80003分钟取上清

3..样本保存--如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

人αN已酰氨基葡糖苷酶(αNAG)ELISA试剂盒现货以上样本采集及处理方式供您参考,如有问题请来电,齐一生物销售:0216034 8496181214 53965173021 04490

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