检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等

检测方法：ELISA

检测类型：酶联免疫夹心法

产品的用途：仅供科研究课题使用

价格及详细资料：齐一生物销售：021－6034 8496；181214 53965；173021 04490；.齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供优质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.

【玉米赤霉醇检测试剂盒】实验原理：

   本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.

试剂盒组成：

齐一生物销售：021－6034 8496；181214 53965；173021 04490

【玉米赤霉醇检测试剂盒】标本要求：

1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.

操作步骤：

1.     标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在、第二孔中分别加标准品100μl,然后在、第二孔中加标准品稀释液50μl,混匀；然后从孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【玉米赤霉醇检测试剂盒】

2.     加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.

3.     温育：用封板膜封板后置37℃温育30分钟.

4.     配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.

5.     洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.

6.     加酶：每孔加入酶标试剂50μl,空白孔除外.

7.     温育：操作同3.

8.     洗涤：操作同5.

9.     显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.

10. 终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.

11. 测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.

注意事项：

1． 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.

2． 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果. 【玉米赤霉醇检测试剂盒】

3． 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.

4． 请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请后乘以总稀释倍数（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物请避光保存.

7． 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.

8． 所有样品,洗涤液和各种废弃物都应按传染物处理.

9． 本试剂不同批号组分不得混用.

10. 如与英文说明书有异,以英文说明书为准.

计算：

以标准物的浓度为横坐标,OD值为纵坐标,   

在坐标纸上绘出标准曲线,根据样品的OD     

值由标准曲线查出相应的浓度；再乘以稀释     

倍数；或用标准物的浓度与OD值计算出标     

准曲线的直线回归方程式,将样品的OD值     

代入方程式,计算出样品浓度,再乘以稀释

倍数,即为样品的实际浓度.

试剂盒性能：

1.样品线性回归与预期浓度相关系数R值为0.95以上.

2.批内与批见应分别小于9%和11%    

保存条件及有效期：

1.试剂盒保存；2-8℃.

2．有效期：6个月

301005     Attractene Transfection Reagent (1 ml)           CNY                             

9012675  Peristaltic Pump, 4 channel (A04897)               CNY                              【玉米赤霉醇检测试剂盒】

9012671  Cable, PCON98 to release valve, BR8000                  CNY                             

9002029  Rotor-Gene Q MDx 5plex, PrioPLUS         "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Regional Repair Center service, 7-10 day turn around time

Loaner available in 2 days*

Annual Inspections (1PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9002019  Rotor-Gene Q MDx 2plex HRM, PrioPLUS       "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Loaner available in 2 days*

Annual Inspections (1 onsite PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9002002  Rotor-Gene Q MDx 2plex Platform          CNY                             

970540     UGT1A1 Pyro Kit (24)                CNY                             

293025     mericon Corn (96)             CNY                             

293015     mericon Soy Kit (96)                   CNY                             

929525     QX Alignment Marker 50bp/500bp (1.5 ml)              CNY                             

206241     Type-it Microsatellite PCR Kit (70)   "The Type-it Microsatellite PCR Kit is developed for fast ａnd reliable genotypic analysis of humans, animals, plants, ａnd bacteria using

microsatellites, STR, or VNTR markers without the need for lengthy optimization procedures

Features

 Reliable microsatellite analysis by multiplex PCR

 Microsatellite assay development without optimization

 Successful ａnd specific coamplification of all fragments

 Optimized protocols for any detection platform"       CNY                             

291524     mericon Quant MON 810 (48)          CNY                             

9001904  QIAgility HEPA/UV (PC) PrioPLUS     "QIAgility HEPA/UV (PC)Instrument

Installation of QIAgility instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9001899  QIAxtractor, UV/HEPA (PC) Priority QIAxtractor, UV/HEPA (PC) Priority includes the QIAxtractor instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service ａnd inspection is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.        CNY                             

133124     NeXtalStock TRIS pH 8.5 (200)                   CNY                             

965942     QIAamp Investigator BioRobot Kit (12)            CNY                             

133191     NeXtalStock D-(+)-Sucrose (200)               CNY                             

133184     NeXtalStock Citric acid pH 3.6 (200)                  CNY                              【玉米赤霉醇检测试剂盒】

9018472  Set, O-rings (10), QIAC              CNY                             

133101     NeXtalStock Potassium sulfate (200)                 CNY                             

133091     NeXtalStock PEG 750 MME (200)             CNY                             

9001867  Rotor-Gene Q 5plex HRM (PC) Priority    Rotor-Gene Q 5plex HRM (PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service ａnd response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                            

9012422  Worktable, complet, BR9604/B                 CNY                             

9012390  Wash station, 4-probes v2.01           CNY                             

205113     Omniscript RT Kit (200)   For 200 reverse-transcription reactions: 800 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water         CNY                             

133167     NeXtalStock CAPSO pH 9.0 (200)               CNY                             

9018429  Key, replacement, green, QX            CNY                             

290125     mericon Listeria spp (96)          CNY                             

9015404  Board, IST, M96                 CNY                             

9001751  QIAsymphony SP System QIAsymphony SP System combines the QIAsymphony SP instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9012284  Power supply (8A), color code 9170                   CNY                             

38055       LCH Cartridge (5ml)          CNY                             

4503303  artus CMV QS-RGQ Kit (24) RUO               CNY                             

NG0395   乙酰胆碱转移酶ChAT检测试剂盒       1管 比色法                                

NG0396   丁酰胆碱酯酶检测试剂盒     1管 比色法                                

NG0397   葡萄糖-6-磷酸脱氢酶G－6－PD检测试剂盒      1管 比色法                                

NG0398   b－N－乙酰氨基葡萄糖苷酶NAG检测试剂盒   1管 比色法                                

NG0399   丙酮酸检测试剂盒         1管 比色法                                

NG0400   丙酮酸激酶PK检测试剂盒   1管 比色法                                

NG0401   己糖激酶HK检测试剂盒       1管 比色法                                

NG0402   醛缩酶ALD检测试剂盒         1管 比色法                                

NG0403   胃蛋白酶检测试剂盒     1管 比色法                                 【玉米赤霉醇检测试剂盒】

NG0404   胰蛋白酶检测试剂盒     1管 比色法                                

NG0405   碱性磷酸酶AKP检测试剂盒         1管 比色法                                

NG0406   酸性磷酸酶ACP检测试剂盒         1管 比色法                                

NG0407   前列腺酸性磷酶PACP检测试剂盒       1管 比色法                                

NG0408   谷氨酰胺合成酶GS检测试剂盒  1管 比色法                                

NG0409   腺苷脱氨酶ADA检测试剂盒        1管 比色法                                

NG0410   唾液酸SA检测试剂盒   1管 比色法                                

NG0411   细胞凋亡TdT介导的原位末端切口平移双标记法检测试剂盒 1片 TUNEL法                                     

NG0412   维生素EVE检测试剂盒         1管 比色法                                

NG0413   维生素CVC检测试剂盒         1管 比色法                                

NG0414   维生素B1VB1检测试剂盒    1管 比色法                                

NG0415   维生素B2VB2检测试剂盒    1管 比色法                                

NG0416   总氨基酸T－AA检测试剂盒         1管 比色法                                

NG0417   苹果酸脱氢酶检测试剂盒     1管 紫外比色法                                

NG0418   羟脯氨酸样本前处理     1管                                      

NG0419   羟脯氨酸Hyp检测试剂盒     1管 比色法                                 【玉米赤霉醇检测试剂盒】

NG0420   D－木糖检测试剂盒      1管 比色法                                

NG0421   糖化血清蛋白GSP检测试剂盒     1管 比色法                                

NG0422   糖化血红蛋白GHb检测试剂盒    1管 比色法                                

NG0423   总脂酶检测试剂盒         1管 比色法                                

NG0424   肝脂酶/脂蛋白脂酶检测试剂盒   1管 比色法                                

NG0425   肝/肌糖元检测试剂盒   1管 比色法                                

NG0426   脂褐质不包括比色检测试剂盒     1管 荧光比色法                                

NG0427   脂褐质包括比色检测试剂盒         1管 荧光比色法                                

     稀释倍数：2.0

7）尿样

（1）    取3ml尿样，4000g离心5min。

（2）    离心后称取1ml，加入0.5mL1×样品提取液，3.5mL双蒸水，0.5mL 1M HCL和40µL 50 mM 2-硝基苯甲醛，剧烈振荡1分钟；

（3）    50°C恒温培养3小时；（说明书后的**标记方法可供选择）

（4）    加入5mL 0.1 M K₂HPO₄，0.4 mL 1 M NaOH ， 6 mL 乙酸乙酯，转速振荡1min；

（5）    室温下4000g离心10分钟；

（6）    取3mL上清液(包含了0.5g的原始样品) 到另一试管（避免触及下层水相！如果移取液中含有水相，再次4000g离心5分钟，取上层有机相），在60 -70°C减压蒸馏或60-70°C氮气吹干；

（7）    用1 mL 正己烷(或者正庚烷)溶解干燥好的样品；

（8）    加入1mL1×样品提取液，剧烈振荡2分钟；

（9）    室温下4000g离心10分钟；

（10）    每孔取100 µL下清液，进行样品测试。

稀释倍数：2.0

检测步骤

试剂的准备

注意：试剂在使用之前要在室温下解冻1-2小时，确定在使用前阅读过注意事项。准备适量的检测用试剂，所有的试剂摇匀后使用。准备足够所有小管所需的用量，不能将试剂倒回原来的瓶子中，处理试剂时建议用废弃的瓶子以降低污染的风险。

1x洗液的制备

1体积的20x洗液同19体积的蒸馏水混合

检测

\以下为计算份量的表格，用户可根据自己的需要来确定需要配置多少试剂。

（1）    加入100mL的标准品于所设定的孔中；

（2）    加入100mL的样品于所设定的孔中

（3）    在每孔中加入50mL 1×SEM-HRP，轻敲微孔板边缘混匀1分钟；

（4）    室温（22.5±2.5）°C避光孵育30分钟；

（5）    洗板3次，每次应该加入250mL 1×洗液，后一次使板尽量甩干并在吸水纸上吸干；

（6）    每孔加入100mL的TMB底物；

（7）    在室温（22.5±2.5）°C避光孵育20分钟后，每孔加入100mL的终止液终止反应;

（8）    在450nm或450/650nm下读OD值 (读数前，用吸水纸将板底部的指痕和水分擦去，避免影响读数)。

10．结果计算【群勃龙检测试剂盒】

（1）    分别计算标准、质控和样品的平均吸光度值和相对吸光度值。

相对吸光度值 (%) = (标准或样品吸光度值/零标准吸光度值) ´ 100

（2）    以相对吸光度值为纵坐标，标准浓度为横坐标建立标准曲线。

（3）    从标准曲线上读取质控和样品的浓度值。

（4）    样品检测下限计算方法如下：

样品检测下限 = (0.025ng/g) ´ (稀释倍数)

样品定量下限 = (0.1ng/g) ´ (稀释倍数)

例如，肉类样品的稀释倍数是2.0,那么肉类样品的检测下限是0.05ppb,定量检测下限是0.2ppb。

800090     Ready-Run Rxns.               CNY                             

765312     PAXgene TissueFIX Cont. (50 ml) Kit                  CNY                             

132009     EasyXtal 15-Well Tool X-Seal (100)   "100 empty 15-well crystallization plates with X-Seal crystallization supports (5 boxes of 20)

For manual setup of hanging ｄrop crystallization experiments."    CNY                              【群勃龙检测试剂盒】

762931     Buffer BM3 Wash buffer 8 x 150 ml conc.                 CNY                             

204054     QuantiFast SYBR Green PCR Kit (400)               CNY                             

203741     QIAGEN Fast Cycling PCR Kit (50)    "Trial Kit: For 50 x 20 µl reactions: 1 x 0.5 ml Fast Cycling Master Mix (2x), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution

-to be used as sample Kit"        CNY                             

159340     Sterile Foam-Tipped Swabs (100)              CNY                             

158906     Cell Lysis Solution (125 ml)                 CNY                             

35061       Ni-NTA HisSorb Plates (5)          5 Ni-NTA-coated, transparent 96-well plates  CNY                             

678891     ipsogen MLL-DUP e2e8 Standards            CNY                             

678591     ipsogen MLL-AF9 e8e10 Standards           CNY                             

678191     ipsogen MLL-AF4 e10e4 Standards           CNY                             

34460       Penta.His HRP Conjugate Kit   125 ul Penta-His HRP Conjugate, 5 g Blocking Reagent, 50 ml Blocking Reagent Buffer (10x concentrate)    CNY                             

32746       EasyXpress Linear Template Fab Kit (100)                 CNY                             

32501       EasyXpress Protein Synthesis Kit (5)         "- Kit for cell-free protein expression in E.coli cell-free extracts.

- The same as the EasyXpress Protein Synthesis Kit (20) (former EasyXpress Protein Synthesis Mini Kit), but only 5 instead of 20 reactions

- Ideal for testing ａnd expression of QIAgenes"       CNY                             

30900       Glutathione HiCap Matrix (10 ml)             CNY                             

28181       QIAquick 96 PCR Purification Kit (4)         For purification of 4 x 96 PCR reactions: 4 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), Caps        CNY                             

9011595  Tip adapter, 300 µl, 120 mm, v2.04                  CNY                             

9011565  Shaker, 4-plate for Plato 7                 CNY                             【群勃龙检测试剂盒】

9013185  Tubing, waste, Dispense           CNY                             

9013174  Connector, tubing, 1-8, BR8000                CNY                             

9013169  Tubing, syringe inlet 1, BR8000                 CNY                             

9011433  BGR Z-Motor P1300 Spez. MWG              CNY                             

9011422  Tip Rack Holder, RCS1               CNY                             

9243119  Green Laser Contr. Coherent 115M-13, LC               CNY                             

9243556  HC2, Full Agreement        "Service response time (on-site repair):not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage depot repair (parts, labor, packaging, shipping): yes, full coverage

For defect DML, after customer calling QIAGEN technical service hotline before 12 am:

- A special package containing a replacement unit shall be sent to the customer within 2 working days.

- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.  

For DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer ａnd Votexer:

- A new unit shall be sent to customer within  7-10 working days at no extra cost

- Customer shall send the defect unit to QIAGEN for disposal

- On-site installation of PC ａnd application software is not covered by the agreement

The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines ａnd documented according to GLP / GMP"    CNY                             

101.901-24       #HLA-DQB1*0602; DQA1*0102 incl.Taq (24)           CNY                             

9241033  #Tube insert, 11 mm, sample carr            CNY                             

9242760  #Warranty PLUS 1, Prem/RRL, 1PM,QSYM RGQ              CNY                             

677491     ipsogen NPM1 mut A gDNA MutaQuant Stds          CNY                             

9011164  Roller, deflection, X-drive, BR8000           CNY                             

9010980  BGR 1-er Shaker PK80 (1177)           CNY                             

133215     NeXtalStock MMT pH 9.0 (200)                 CNY                             

NG0361   Xmol/L草酸     6元/100ml                                           

NG0362   Hank’s液      24元/200ml                                         

NG0363   2,4－二硝基苯肼   8元/100ml                                           

NG0364   4.0mol/L氢氧化钠 8元/100ml                                           

NG0365   任氏液     6元/100ml                                           

NG0366   超氧化物歧化酶SOD检测试剂盒         1管 比色法                                

NG0367   丙二醛MDA检测试剂盒       1管 比色法                                

NG0368   黄嘌呤氧化酶XOD检测试剂盒    1管 比色法                                

NG0369   总抗氧化能力T－AOC检测试剂盒      1管 比色法                                

NG0370   羟自由基检测试剂盒     1管 比色法                                 【群勃龙检测试剂盒】

NG0371   髓过氧化物酶MPO检测试剂盒   1管 比色法                                

NG0372   过氧化氢酶CAT检测试剂盒          1管 紫外比色法                                

NG0373   谷胱甘肽－S转移酶GSH－ST检测试剂盒 1管 比色法                                

NG0374   谷胱甘肽过氧化物酶GSH－PX检测试剂盒         1管 比色法                                

NG0375   氧化型谷胱甘肽GSSG检测试剂盒       1管 比色法                                

NG0376   谷胱甘肽还原酶GR检测试剂盒  1管 比色法                                

NG0377   还原型谷胱甘肽GSH检测试剂盒         1管 比色法                                

NG0378   巯基-SH检测试剂盒       1管 比色法                                

NG0379   单胺氧化酶MAO检测试剂盒       1管 紫外比色法                                

NG0381   一氧化氮NO硝酸还原酶法检测试剂盒      1管 比色法                                

NG0382   一氧化氮合成酶诱导型检测试剂盒     1管 比色法                                

NG0383   一氧化氮合成酶总NOS检测试剂盒    1管 比色法                                

NG0384   乳酸LD血清、组织检测试剂盒  1管 比色法                                

NG0385   乳酸LD全血检测试剂盒       1管 比色法                                

NG0386   乳酸脱氢酶LDH检测试剂盒         1管 比色法                                

NG0387   ATP酶钠钾、钙镁ATP酶检测试剂盒  1管 比色法                                 【群勃龙检测试剂盒】

NG0388   钠钾、钙镁ATP酶中任一项检测试剂盒     1管 比色法                                

NG0389   超微量ATP酶检测试剂盒     1管 比色法                                

NG0390   钠钾、钙镁ATP酶中任一项检测试剂盒                                                   

NG0391   琥珀酸脱氢酶SDH检测试剂盒    1管 比色法                                

NG0392   肌酸激酶CK检测试剂盒       1管 比色法                                

NG0393   胆碱酯酶CHE检测试剂盒    1管 比色法                                

NG0394   乙酰胆碱酯酶T－CHE检测试剂盒       1管 比色法