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齐一生物科技(上海)有限公司

产品介绍

微囊藻毒素/节球藻毒素(DM)检测试剂盒

价 格:¥电议

型 号:

产品完善度:

生产地:其他访问量:24次

发布日期:2016/8/21 14:06:30

更新日期:2016/8/21 14:06:30

详细内容

 齐一生物销售:0216034 8496181214 53965173021 04490

试剂盒名称:微囊藻毒素/节球藻毒素(DM)检测试剂盒

格:96T

产品的用途:仅供科研究使用!

价格:电议,或咨询在线客服QQ,或者以邮件形式发到我司邮箱,齐一生物科技(上海)有限公司提供大量品牌进口原装,分装,以及稳定性强的国产ELISA试剂盒,性价比极高。欢迎您前来选购!

本试剂盒主要用于各种动物肌肉组织、肝脏、尿液、血清、饲料等中 残留定量检测!

 

微囊藻毒素/节球藻毒素(DM)检测试剂盒试验原理:
该产品是固相夹心法酶联免疫吸附实验(ELISA.已知待测物质浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测。先将待测物质和生物素标记的抗体同时温育。洗涤后,加入亲和素标记过的HRP。再经过温育和洗涤,去除未结合的酶结合物,然后加入底物AB,和酶结合物同时作用。产生颜色。颜色的深浅和样品中待测物质的浓度呈比例关系。

试剂盒内容及其配制

 

试剂盒自备材料
1
)蒸馏水。
2
)加样器:5ul10ul50ul100ul200ul500ul1000ul
3
)振荡器及磁力搅拌器等。

检测试剂盒安全性
1
)避免直接接触终止液和底物AB。一旦接触到这些液体,请尽快用水冲洗。
2
)实验中不要吃喝、抽烟或使用化妆品。
3
)不要用嘴吸取试剂盒里的任何成份

 

操作注意事项
1
)试剂应按标签说明书储存,使用前恢复到室温。稀稀过后的标准品应丢弃,不可保存。
2
)实验中不用的板条应立即放回包装袋中,密封保存,以免变质。
3
)不用的其它试剂应包装好或盖好。不同批号的试剂不要混用。保质前使用。
4
)使用一次性的吸头以免交叉污染,吸取终止液和底物AB液时,避免使用带金属部分的加样器。
5
)使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。
6
)洗涤酶标板时应充分拍干,不要将吸水纸直接放入酶标反应孔中吸水。
7
)底物A应挥发,避免长时间打开盖子。底物B对光敏感,避免长时间暴露于光下。避免用手接触,有毒。实验完成后应立即读取OD值。
8
)加入试剂的顺序应一致,以保证所有反应板孔温育的时间一样。
9
)按照说明书中标明的时间、加液的量及顺序进行温育操作。

 

样品收集、处理及保存方法
1
)血清-----操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后,1000×g离心10分钟将血清和红细胞迅速小心地分离。
2
)血浆-----EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3
)细胞上清液---1000×g离心10分钟去除颗粒和聚合物。
4
)组织匀浆-----将组织加入适量生理盐水捣碎。1000×g离心10分钟,取上清液
5
)保存------如果样品不立即使用,应将其分成小部分-70 ℃保存,避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒,检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

微囊藻毒素/节球藻毒素(DM)检测试剂盒

试剂盒试剂的准备
1
)标准品:标准品的系列稀释应在实验时准备,不能储存。稀释前将标准品振荡混匀。
2
)洗涤缓冲液(50×)的稀释:蒸馏水50倍稀释。

 

检测试剂盒操作步骤
1
)使用前,将所有试剂充分混匀。不要使液体产生大量的泡沫,以免加样时加入大量的气泡,产生加样上的误差。
2
)根据待测样品数量加上标准品的数量决定所需的板条数。每个标准品和空白孔建议做复孔。每个样品根据自己的数量来定,能使用复孔的尽量做复孔。标本用标本稀释液11稀释后加入50ul于反应孔内。
3
)加入稀释好后的标准品50ul于反应孔、加入待测样品50ul于反应孔内。立即加入50ul的生物素标记的抗体。盖上膜板,轻轻振荡混匀,37℃温育1小时。
4
)甩去孔内液体,每孔加满洗涤液,振荡30秒,甩去洗涤液,用吸水纸拍干。重复此操作3次。如果用洗板机洗涤,洗涤次数增加一次。
5
)每孔加入80ul的亲和链酶素-HRP,轻轻振荡混匀,37℃温育30分钟。
6
)甩去孔内液体,每孔加满洗涤液,振荡30秒,甩去洗涤液,用吸水纸拍干。重复此操作3次。如果用洗板机洗涤,洗涤次数增加一次。
7
)每孔加入底物AB50ul,轻轻振荡混匀,37℃温育10分钟。避免光照。
8
)取出酶标板,迅速加入50ul终止液,加入终止液后应立即测定结果。
9
)在450nm波长处测定各孔的OD值。

 

检测试剂盒局限
6
号标准品以上的结果为非线性的,根据此标准曲线无法得到精确的结果。

 

微囊藻毒素/节球藻毒素(DM)检测试剂盒性能
1.
灵敏度:小的检测浓度小于1号标准品。稀释度的线性。样品线性回归与预期浓度相关系数R值为0.990
2.
特异性:不与其它细胞因子反应。
3.
重复性:板内、板间变异系数均小于10%

检测试剂盒结果判断与分析
1
、仪器值:于波长450nm的酶标仪上读取各孔的OD
2
、以吸光度OD值为纵坐标(Y),相应的待测物质标准品浓度为横坐标(X),做得相应的曲线,样品的待测物质含量可根据其OD值由标准曲线换算出相应的浓度。

 

 齐一生物科技有限公司主要经营进口原装Elisa试剂盒,种类齐全,价格实惠,购买我公司产品的新老客户,可享免费代测实验服务,可检测人源,小鼠,大鼠,猪,狗,猴,兔,羊,鸡,牛,鸭子,鱼,植物等各种样本。全程提供技术支持。如有什么问题请给我们电话或者留言,告诉我您的需要,并留下您宝贵的意见和建议。

 

 

 

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DQYC27 农残速测试剂       农残速测试剂                                  

DQYC28 农残速测试剂(国标)       农残速测试剂(国标)                                  

DQYC29 "农残速测增强剂

"      "农残速测增强剂

"                                 

DQYQYC30   农药残留速测卡 农药残留速测卡                              

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QYDC34 亚硝酸盐快速检测试剂盒   100/                                  

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e='��&-a�2�_1��;Times New Roman";Times New Roman"'>粮食作物(玉米、大米、豆类、花生、麦类等)、、饲料等                        

 

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DQYC21 茶叶农药速测卡    茶叶农药速测卡                               

DQYC22 "粮食农药速测卡

"      "粮食农药速测卡

"                                 

DQYC23 蔬菜农药检测卡    蔬菜农药检测卡                               

DQYC24 高灵敏度农药速测卡    高灵敏度农药速测卡                               

DQYC25 农残速测试剂       农残速测试剂                                   

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