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试剂盒名称：微囊藻毒素/节球藻毒素(DM)检测试剂盒

规 格：96T

产品的用途：仅供科研究使用！

价格：电议,或咨询在线客服QQ,或者以邮件形式发到我司邮箱，齐一生物科技（上海）有限公司提供大量品牌进口原装，分装，以及稳定性强的国产ELISA试剂盒，性价比极高。欢迎您前来选购！

本试剂盒主要用于各种动物肌肉组织、肝脏、尿液、血清、饲料等中 残留定量检测！

 

微囊藻毒素/节球藻毒素(DM)检测试剂盒试验原理：
该产品是固相夹心法酶联免疫吸附实验（ELISA）.已知待测物质浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测。先将待测物质和生物素标记的抗体同时温育。洗涤后，加入亲和素标记过的HRP。再经过温育和洗涤，去除未结合的酶结合物，然后加入底物A、B，和酶结合物同时作用。产生颜色。颜色的深浅和样品中待测物质的浓度呈比例关系。

试剂盒内容及其配制

 

试剂盒自备材料
1）蒸馏水。
2）加样器：5ul、10ul、50ul、100ul、200ul、500ul、1000ul。
3）振荡器及磁力搅拌器等。

检测试剂盒安全性
1）避免直接接触终止液和底物A、B。一旦接触到这些液体，请尽快用水冲洗。
2）实验中不要吃喝、抽烟或使用化妆品。
3）不要用嘴吸取试剂盒里的任何成份

 

操作注意事项
1）试剂应按标签说明书储存，使用前恢复到室温。稀稀过后的标准品应丢弃，不可保存。
2）实验中不用的板条应立即放回包装袋中，密封保存，以免变质。
3）不用的其它试剂应包装好或盖好。不同批号的试剂不要混用。保质前使用。
4）使用一次性的吸头以免交叉污染，吸取终止液和底物A、B液时，避免使用带金属部分的加样器。
5）使用干净的塑料容器配置洗涤液。使用前充分混匀试剂盒里的各种成份及样品。
6）洗涤酶标板时应充分拍干，不要将吸水纸直接放入酶标反应孔中吸水。
7）底物A应挥发，避免长时间打开盖子。底物B对光敏感，避免长时间暴露于光下。避免用手接触，有毒。实验完成后应立即读取OD值。
8）加入试剂的顺序应一致，以保证所有反应板孔温育的时间一样。
9）按照说明书中标明的时间、加液的量及顺序进行温育操作。

 

样品收集、处理及保存方法
1）血清－－－－-操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后，1000×g离心10分钟将血清和红细胞迅速小心地分离。
2）血浆－－－－-EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3）细胞上清液－－-1000×g离心10分钟去除颗粒和聚合物。
4）组织匀浆－－－－-将组织加入适量生理盐水捣碎。1000×g离心10分钟，取上清液
5）保存－－－－－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【微囊藻毒素/节球藻毒素(DM)检测试剂盒】

试剂盒试剂的准备
1）标准品：标准品的系列稀释应在实验时准备，不能储存。稀释前将标准品振荡混匀。
2）洗涤缓冲液（50×）的稀释：蒸馏水50倍稀释。

 

检测试剂盒操作步骤
1）使用前，将所有试剂充分混匀。不要使液体产生大量的泡沫，以免加样时加入大量的气泡，产生加样上的误差。
2）根据待测样品数量加上标准品的数量决定所需的板条数。每个标准品和空白孔建议做复孔。每个样品根据自己的数量来定，能使用复孔的尽量做复孔。标本用标本稀释液1：1稀释后加入50ul于反应孔内。
3）加入稀释好后的标准品50ul于反应孔、加入待测样品50ul于反应孔内。立即加入50ul的生物素标记的抗体。盖上膜板，轻轻振荡混匀，37℃温育1小时。
4）甩去孔内液体，每孔加满洗涤液，振荡30秒，甩去洗涤液，用吸水纸拍干。重复此操作3次。如果用洗板机洗涤，洗涤次数增加一次。
5）每孔加入80ul的亲和链酶素-HRP，轻轻振荡混匀，37℃温育30分钟。
6）甩去孔内液体，每孔加满洗涤液，振荡30秒，甩去洗涤液，用吸水纸拍干。重复此操作3次。如果用洗板机洗涤，洗涤次数增加一次。
7）每孔加入底物A、B各50ul，轻轻振荡混匀，37℃温育10分钟。避免光照。
8）取出酶标板，迅速加入50ul终止液，加入终止液后应立即测定结果。
9）在450nm波长处测定各孔的OD值。

 

检测试剂盒局限
6号标准品以上的结果为非线性的，根据此标准曲线无法得到精确的结果。

 

微囊藻毒素/节球藻毒素(DM)检测试剂盒性能
1. 灵敏度：小的检测浓度小于1号标准品。稀释度的线性。样品线性回归与预期浓度相关系数R值为0.990。
2. 特异性：不与其它细胞因子反应。
3. 重复性：板内、板间变异系数均小于10%。

检测试剂盒结果判断与分析
1、仪器值：于波长450nm的酶标仪上读取各孔的OD值
2、以吸光度OD值为纵坐标（Y），相应的待测物质标准品浓度为横坐标（X），做得相应的曲线，样品的待测物质含量可根据其OD值由标准曲线换算出相应的浓度。

 

 齐一生物科技有限公司主要经营进口原装Elisa试剂盒，种类齐全，价格实惠，购买我公司产品的新老客户，可享免费代测实验服务，可检测人源，小鼠，大鼠，猪，狗，猴，兔，羊，鸡，牛，鸭子，鱼，植物等各种样本。全程提供技术支持。如有什么问题请给我们电话或者留言，告诉我您的需要，并留下您宝贵的意见和建议。

 

 

 

9240984  PCB, p&c, cooling device, Qsym         CNY                           

9240983  Cooling device, eluate drawer, Qsym           CNY                           

9240952  Handler, Qsym              CNY                           

9240950  PCB, xyz, portal, Qsym        CNY                           

9240836  QIAcube, Full Agreement, no PM  "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) or PC repair provision: not included

Transportation cost coverage: not applicable"      CNY                           

9240684  Pogo Stick Kit, AP196/98             CNY                           

9240802  Performance, IQ/OQ, EZ1           CNY                           

9240782  Update, Weigh Cell, AP196           CNY                           

9240779  500 ml Reagent #6 Update, AP98         CNY                           

9240770  Assy, Capper Shuttle, AP96          CNY                           

9240767  8 OUT Relay, F2-08TRS, AP96           CNY                           

9240765  32 IN Module, D2-32ND3, AP96         CNY                           

9241778  RGQ, Inspection Service       "The Annual Inspection Service, Rotorgene-Q is a Regional Repair Center equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the Rotorgene are inspected ａnd tested ａnd an OTV check is conducted. Performed tests ａnd test results are documented in a GMP/GLP-compliant Report."     CNY                           

9241730  BR Universal ApplicationPack Veterinary             CNY                           

9241394  C-Nut set, EZ1a            CNY                            【微囊藻毒素/节球藻毒素(DM)检测试剂盒】

9240749  Belt, Shaker, AP            CNY                           

9243644  Guided Valid Support, QuantiPlex/OPHyres  The QIAGEN Quantiplex/Quantiplex HYres Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the Quantiplex/Quantiplex HYres chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on operation to match the lab’s forensic workflow. The Guided Validation service includes experimental design consulation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  CNY                           

9243642  Pyro Q24, Repair Fee    "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9243540  LiquiChip, PM Service   "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9243114  Flow Control Valve, Weigh Cell, AP             CNY                           

9242862  QIArent&go - Rent out fee QSSP Cabinet           CNY                           

9242676  QS RGQ, Installation     The Installation, QIAsymphony RGQ facilitates the quick implementation of the QIAsymphony RGQ into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of QIAsymphony RGQ instruments ａnd provides the user with an introductory training. The installation includes or labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.     CNY                           

5000-1324      Motor 230V, SKR1        CNY                           

206045    Type-it Fast SNP Probe PCR Kit (800) "Features

 Outstanding separation for reliably high call rates

 For difficult SNP loci ａnd for low amounts of template

 Validated using TaqMan SNP Genotyping Assays

 Up to 40% time savings due to fast-cycling procedure

 Convenient ｍaster mix format ａnd optimized protocol"  CNY                           

9001911  PyroMark Q24 MDx, Priority       PyroMark Q24 MDx, Priority includes the Pyromark Q24 MDx system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service ａnd response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. (In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)   CNY                           

965842    QIAamp 96 DNA Swab BioRobot Kit (12)    For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Proteinase K, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps   CNY                           

133186    NeXtalStock Citric acid pH 4.0 (200)          CNY                           

133185    NeXtalStock Citric acid pH 3.8 (200)          CNY                           

9018554  Cover, front, left, EZ1a         CNY                           

9018529  Communicator, Software CD, EZ1a            CNY                           

51206     FlexiGene DNA Kit (250)      For purification of DNA from 250 ml whole blood: Buffers, QIAGEN Protease CNY                            【微囊藻毒素/节球藻毒素(DM)检测试剂盒】

51106      QIAamp DNA Blood Mini Kit (250)      For 250 DNA minipreps: 250 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)  CNY                           

50214     QIAamp UCP Pathogen Mini Kit (50),G              CNY                           

DQYC26 农残速测试剂（行标）       农残速测试剂（行标）                                  

DQYC27 农残速测试剂       农残速测试剂                                  

DQYC28 农残速测试剂（国标）       农残速测试剂（国标）                                  

DQYC29 "农残速测增强剂

"      "农残速测增强剂

"                                 

DQYQYC30   农药残留速测卡 农药残留速测卡                              

RT089     "DNA提取试剂盒

"      100次/盒                                   【微囊藻毒素/节球藻毒素(DM)检测试剂盒】

RT090     转基因玉米成分PAT检测试剂盒       100次/盒                                  

RT091     "转基因大豆成分CP4-EPSPS检测试剂盒

"      100次/盒                                  

QYDC31 甲醛快速检测试剂盒   100次/盒                                  

QYDC32 吊白块快速检测试剂盒      100次/盒                                  

QYDC33 二氧化硫快速检测试剂盒   100次/盒                                  

QYDC34 亚硝酸盐快速检测试剂盒   100次/盒                                  

QYDC35 硼砂快速检测试剂盒   100次/盒                                  

QYDC36 双氧水快速检测试剂盒      100次/盒                                  

QYDC37 工业碱快速检测试剂盒       100次/盒                                  

QYDC38 硝酸盐快速检测试剂盒      50次/盒                             

QYDC39 甲醇检测试剂盒    50次/盒                             

QYDC40 奶粉蛋白质含量速测盒       50次/盒                             

QYDC41 液态奶新鲜度速测盒    50次/盒                             

QYDC42 过氧化苯甲酰速测盒    50次/盒                             

QYDC43 面粉中铝快速检测试剂盒    50次/盒                             

QYDC44 孔雀石绿筛查柱检测试剂盒       10次/盒                              【微囊藻毒素/节球藻毒素(DM)检测试剂盒】

QYDC45 孔雀石绿组织检测试剂盒    10次/盒                             

QYDC46 苏丹红快速检测试剂盒      50次/盒                             

QYDC47 水溶性非食用色素速测盒    50次/盒                             

QYDC48 食用色素速测盒    50次/盒                             

QYDC49 肉类水份检测卡    60次/盒                             

DQYC50 糖精钠速测盒       50次/盒                             

DQYC51 肉类新鲜度速测盒       50次/盒                             

DQYC52 食醋中游离矿酸速测试纸    40片/盒                             

DQYC53 食醋中总酸快速测定试液    25次/盒                             

DQYC54 酱油总酸氨基酸态氮速测液       20份/瓶                             

DQYC55 假冒伪劣味精速测试液包   20次/盒                             

e=＇��&-a�2�_1��;Times New Roman";Times New Roman"＇>粮食作物（玉米、大米、豆类、花生、麦类等）、、饲料等                        

 

QY173    呕吐毒素检测试剂盒    96孔/盒 粮食作物（玉米、大米、豆类、花生、麦类等）、饲料等                         

QY174    微囊藻毒检测试剂盒    96孔/盒 适用于水样、蓝藻、鱼肉等样品                           

DQYC20 蔬菜农药速测卡    蔬菜农药速测卡                               

DQYC21 茶叶农药速测卡    茶叶农药速测卡                               

DQYC22 "粮食农药速测卡

"      "粮食农药速测卡

"                                 

DQYC23 蔬菜农药检测卡    蔬菜农药检测卡                               

DQYC24 高灵敏度农药速测卡    高灵敏度农药速测卡                               

DQYC25 农残速测试剂       农残速测试剂