【友情提示】：本产品仅供科研研究使用，不得用于人体临床直接检测。避免给您带来不必要的损失，请仔细阅读购买说明！

本试剂盒仅供研究使用，齐一生物销售：021－6034 8496；181214 53965；173021 04490欢迎选购！

操作步骤

1.准备试剂，样品和标准品

2.加入准备好的样品和标准品，37℃反应30分钟

3.洗板5次，加入酶标试剂，37℃反应30分钟

4.洗板5次，加入显色液A、B，37℃显色15分钟

5.加入终止液

6.15分钟之内读OD值

7.计算
齐一生物供应ELISA试剂盒检测的目的是为实验提供准确可靠的定量分析实验依据。为了保证实验数据的可靠性，在实验过程中必须坚持全面质量控制和全过程质量控制。在收集标本前都必须有一个完整的计划。

【雌二醇(E2)检测试剂盒】样本保存条件：

1.2-8℃冰箱多放2周

2.-20到-50℃的冰箱可以放3-6个月

3.-50℃以下的冰箱，可以放一年

【雌二醇(E2)检测试剂盒】样本收集

1.血清样本用干燥管（红色）或者促凝管（黄色）收集

2.血浆用抗凝管，一般有EDTA抗凝（紫色）、枸橼酸钠抗凝（黑色或者蓝色）

3.动物组织匀浆（细胞）：

              1g组织加入9ml PBS（浓度为0.1M，pH为7.4的）测分泌型指标

              1g组织加入1ml组织裂解液加入8ml PBS 测细胞内

粪便样本：1g样本+9ml PBS（浓度为0.01M，pH为7.4的）

没有PBS的话也可以用一下生理盐水，还是用PBS

【雌二醇(E2)检测试剂盒】样本离心转速及时间

1.试管采集的样本：3000转，离心五分钟取上清

2.离心管或者EP管采集的样本：5000－－8000转3分钟取上清

3..样本保存－－如果样品不立即使用，应将其分成小部分-70 ℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。

【雌二醇(E2)检测试剂盒】以上样本采集及处理方式供您参考，如有问题请来电，齐一生物销售：021－6034 8496；181214 53965；173021 04490

336835    Custom Cignal Finder Reporter Array          CNY                           

336111    Custom ELISArray        CNY                           

940057    BioSprint 96 DNA Blood Kit (384)        CNY                           

334471    EpiTect ChIP OneDay Kit (12)             CNY                           

331260    miScript PreAMP Custom Primer Mix         CNY                           

331001    RT² miRNA qPCR Assay (200)            CNY                           

9010969  Plate stacker, detachable Unit for stacking up to ten 96-well plates; for use with the robotic handling system  CNY                            【雌二醇(E2)检测试剂盒】

9010928  Motor, X, SamTrack, P3xxx        CNY                           

979302    PyroMark Q24 Cartridge (3) CE   For delivery of nucleotides ａnd reagents for use in PyroMark Q24. CE-Marked CNY                           

981303    Rotor-Disc 72 (240)             CNY                           

981005    PCR Tubes, 0.2 ml  (1000)          CNY                           

9012976  Gripper, left, landoplasted, MTP 120            CNY                           

931955    QIAsymphony Certal Vaccine NA Kit (96)           CNY                           

929707    QX Cartridge Stand Cover (1)             CNY                           

133252    NeXtalStock Sodium phosphate pH 7 (200)         CNY                           

929606    QX FA Separation Buffer (40 ml)         CNY                           

9010509  Motor, turntable 1/2 MTP, P3/7            CNY                           

69525     mericon DNA Bacteria Kit (100)           CNY                           

69106     DNeasy Plant Mini Kit (250)  250 DNeasy Mini Spin Columns, 250 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)  CNY                           

211015    QuantiTect Virus Kit (1000)          CNY                           

206674    Type-it CNV SYBR Green PCR +qC Kit (400)            CNY                           

9012763  Liquid Handling Kit Plato 1300             CNY                           

59568     EpiTect Control DNA (1000)        CNY                            【雌二醇(E2)检测试剂盒】

979014    MOTT 16S Primers For PCR              CNY                           

979011    PyroMark Q96 Vacuum Prep Trough (5)     Re-usable plastic troughs for use in all PyroMark Q96 Vacuum Workstations.       CNY                           

59932     EpiTect MethyLight Assay Hs-ONECUT2(100)    "10-fold primer TaqMan probe mix, for real-time MethyLight PCR

For TaqMan probe-based real-time quantification of methylated or unmethylated CpG sites"    CNY                           

59695     EpiTect PCR Control DNA Set (100)           CNY                           

206343    Type-it Mutation Detect PCR Kit (200) "The Type-it Mutation Detect PCR Kit is especially developed for to fast ａnd reliable detection of mutations such as deletions, insertions,and translocations. It is also highly suited for multiplex PCR-based preamplification of SNPs as preparation for genotyping systems like the

SNaPshot Multiplex Kit from ABI

Features

 Successful & reproducible analysis of multiple mutations

 Optimization-free multiplex PCR assay development

 Specific ａnd sensitive coamplification of all fragments

 Optimized protocol for fast ａnd reliable results"      CNY                           

206341    Type-it Mutation Detect PCR Kit (70)   "The Type-it Mutation Detect PCR Kit is especially developed for to fast ａnd reliable detection of mutations such as deletions, insertions,and translocations. It is also highly suited for multiplex PCR-based preamplification of SNPs as preparation for genotyping systems like the

SNaPshot Multiplex Kit from ABI

Features

 Successful & reproducible analysis of multiple mutations

 Optimization-free multiplex PCR assay development

 Specific ａnd sensitive coamplification of all fragments

 Optimized protocol for fast ａnd reliable results"      CNY                           

9012719  Set, Pins, (6 small, 4 large)           CNY                           

929561    QX Size Marker 50 - 800 bp v2.0 (50 µl)            CNY                           

972804    PyroMark Gold Q96 Reagents (5 x 96) "For performing pyrosequencing reaction on the ID (5x 96) ａnd Q96 MD (15x96)

ID needs more reagents"       CNY                           

301705    HiPerFect Transfection Reagent (1 ml)        CNY                           

QYD643  新城疫病毒荧光定量RT-PCR检测试剂盒 48次/盒                             

QYD644  诺沃克病毒荧光定量RT-PCR检测试剂盒 48次/盒                             

QYD645  坂歧肠杆菌荧光定量PCR检测试剂盒      48次/盒                             

QYD646  沙门氏菌属荧光定量PCR检测试剂盒      48次/盒                             

QYD647  单增李斯特氏菌荧光定量PCR检测试剂盒      48次/盒                             

QYD648  大肠埃希菌O157:H7荧光定量PCR检测试剂盒      48次/盒                             

QYD649  金黄色葡萄球菌荧光定量PCR检测试剂盒      48次/盒                             

QYD650  志贺氏菌属荧光定量    48次/盒                             

QYD651  空肠弯曲菌荧光定量PCR检测试剂盒      48次/盒                             

QYD652  小肠结肠炎耶尔森菌荧光定量PCR检测试剂盒      48次/盒                             

QYD653  溶血性链球菌荧光定量PCR检测试剂盒 48次/盒                             

QYD654  霍乱弧菌荧光定量PCR检测试剂盒   48次/盒                             

QYD655  副溶血弧菌荧光定量PCR检测试剂盒      48次/盒                             

QYD656  产毒副溶血弧菌荧光定量PCR检测试剂盒      48次/盒                              【雌二醇(E2)检测试剂盒】

QYD657  创伤弧菌荧光定量PCR检测试剂盒   48次/盒                             

QYD658  口蹄疫O型病毒抗体间接血凝检测试剂盒                                         

QYD659  猪瘟正向间接血凝试验试剂盒    100头份/盒    800                      

QYD660  口蹄疫O型病毒抗体间接血凝检测试剂盒       100头份/盒    600                      

QYD661  盐酸克伦特罗检测试纸条    20份/盒  5元/份    15元/份               

QYD662  盐酸克伦特罗检测试纸条    20份/盒  9元/份    20元/份               

QYD663  莱克多巴胺检测试纸条（尿样）       20份/盒  5元/份    15元/份               

QYD664  莱克多巴胺检测试纸条(组织)     20份/盒  9元/份    20元/份               

QYD665  氯霉素检测试纸条（液态样品）       20份/盒  5元/份    15元/份               

QYD666  氯霉素检测试纸条（组织）       20份/盒  11元/份  25元/份               

QYD667  孔雀石绿检测试纸条（水样）    20份/盒  11元/份  25元/份               

QYD668  孔雀石绿检测试纸条（组织）    20份/盒  22元/份  50元/份               

QYD669  三聚氰胺快速检测试纸卡    10份/盒  15元/份  35元/份               

QYD670  小反刍兽疫检测试纸条       20份/盒  11元/份  25元/份               

QYD671  盐酸克伦特罗和莱克多巴胺       20份/盒  13.5元/份      30元/份               

QYD672  二联快速检测试纸条    20份/盒  13.5元/份  30元/份                         【雌二醇(E2)检测试剂盒】

QYD673  牛结核病抗体检测试剂盒    Elisa 2*96孔                       

QYD674  荷兰赛迪口蹄疫0型2板试剂盒 Elisa 2*96孔                       

QYD675  口蹄疫非结构蛋白抗体检测试剂盒    Elisa 5*96孔