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发布时间:2024/9/14点击次数:17
Product Name | Gab1 Antibody |
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Antibody Type | Primary Antibodies |
Clonality | Monoclonal |
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Isotype | IgG1 |
Clone Number | 1AT1979 |
Host Species | Mouse |
Tested Applications | WB |
WB~~1:1000: | |
Species Reactivity | Human |
Concentration | 1 mg/ml |
Purification |
Alternative Names | More |
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Molecular Weight(MW) | 76616 Da |
Function | Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR) |
Tissue Specificity | This Gab1 antibody is generated from mice immunized with a GST fusion protein encoding full length human GAB1. |
Entrez Gene | 2549 |
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Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42掳C for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37? for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with anti-Gab1 antibody. Data courtesy of Dr. GS Feng at The Burnham Institute.
Rescue of the JNK pathway by expression of wild-type Gab1 in Gab1-/- cells. Gab1-/- cells were transiently transfected with 1, 2, or 4 of the expression construct for human Gab1 cDNA, using the GenePORTER 2 transfection reagent (Gene Therapy Systems Inc.). After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of Gab1 in transfected cells was confirmed by anti-Gab1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, Gab1-/- cells. (Mol. Cell. Biol. 2004 Feb 15;24(4):1531-1539)
Application Notes | WB~~1:1000: |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
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