Moesin antibody , C-term Catalog Number：OM263676_产品说明_ B2B电子商务综合技术文献

发布时间：2024/9/18点击次数：9

Product Name	Moesin antibody , C-term
Antibody Type	Primary Antibodies
Product description	Rabbit Polyclonal antibody to Moesin (moesin)Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin ａnd radixin. ERM proteins appear to function as cross-linkers between plasma membranes ａnd actin-based cytoskeletons. Moesin is localized to filopodia ａnd other membranous protrusions that are important for cell-cell recognition ａnd signaling ａnd for cell movement. [provided by RefSeq] More
Immunogen	Recombinant protein encompassing a sequence within the C-terminus region of human Moesin. The exact sequence is proprietary.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	ICC/IFIHC-PIPWB
	*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity	HumanMouseRat
Predicted Reactivity	Pig, Rhesus Monkey, BovinePig (98%), Rhesus Monkey (100%), Bovine (99%)
Concentration	0.2 mg/ml
Purification	Affinity purified

Gene Synonyms	MSN antibody membrane-organizing extension spike protein antibody moesin antibody
Molecular Weight(MW)	68 kDa (note)
Cellular Localization	Cell membrane; Peripheral membrane protein; Cylasmic side (By similarity) , Cylasm , cytoskeleton (By similarity) , Apical cell membrane; Peripheral membrane protein; Cylasmic side (By similarity) , Cell projection , microvillus membrane; Periphe

Application

Moesin antibody [C2C3], C-term detects MSN protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg BCL-1 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 7.5 % SDS-PAGE Moesin antibody [C2C3], C-term dilution: 1:1000
Application

Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000
Application

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Moesin antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:500.
Application

Moesin antibody [C2C3], C-term detects Moesin protein at cell membrane in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Moesin antibody [C2C3], C-term diluted at 1:500.
Application

Immunoprecipitation of Moesin protein from A431 whole cell extracts using 5 μg of Moesin antibody [C2C3], C-term . Western blot analysis was performed using Moesin antibody [C2C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Positive Control	NT2D1 , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher. :

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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