官方微信:baichuansw2015 服务热线:0571-85363692
发布时间:2024/9/20点击次数:4
Product Name | Bmi1 antibody |
---|---|
Antibody Type | Primary Antibodies |
Product description |
|
Immunogen | Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary. |
Clonality | Polyclonal |
---|---|
Isotype | IgG |
Host Species | Rabbit |
Tested Applications | IPWB |
*Optimal dilutions/concentrations should be determined by the researcher. : | |
Species Reactivity | Human |
Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Cat, Pig, Chicken, Chimpanzee, BovineMouse (96%), Rat (92%), Xenopus laevis (82%), Dog (98%), Cat (99%), Pig (99%), Chicken (94%), Chimpanzee (100%), Bovine (98%) |
Concentration | 1 mg/ml (Please refer to the vial label for the specific concentration) |
Purification | Affinity purified |
Gene Synonyms | More |
---|---|
Target | Bmi1 |
Molecular Weight(MW) | 37 kDa (note) |
Cellular Localization | Nucleus , Cylasm |
Bmi1 antibody detects Bmi1 protein by western blot analysis. A. 30 μg K562 whole cell lysate/extract B. 30 μg THP-1 whole cell lysate/extract 10 % SDS-PAGE Bmi1 antibody dilution: 1:3000
Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP samples: K562 whole cell extract A. 40 μg K562 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of Bmi1 protein by 4 μg Bmi1 antibody 5 % SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Positive Control | K562 , THP-1 |
---|---|
Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. : |
Form | Liquid |
---|---|
Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
解读B2B电子商务综合行业热点、呈现敏感事件、更多独家分析,尽在易推广微信,扫描二维码免费阅读。