CDC47 antibody Catalog Number：OM246421_产品说明_ B2B电子商务综合技术文献

CDC47 antibody Catalog Number：OM246421

发布时间：2024/9/27点击次数：48

Product Profile

Product Name	CDC47 antibody
Antibody Type	Primary Antibodies
Product description	Monoclonal Mouse Antibody to CDC47 / MCM7This antibody reacts with a 80 kD protein known as hCDC47. hCDC47 is a member of MCM family, which allows DNA to replicate once per cell cycle. Expression of hCDC47 protein has been found in the nuclei of proliferative components of normal lymph nodes, bone marrow, epidermis ａnd mucosa. Malignant tumors from several organs contain more nuclear hCDC47-positive cells than their normal counterparts. This antibody cross reacts with mouse, rat, dog ａnd Xenopus. More
Immunogen	BALB/C mice were injected with recombinant human CDC47 protein.

Key Feature

Clonality	Monoclonal
Isotype	IgG1
Clone Number	47DC141
Host Species	Mouse
Tested Applications	IHC-PIPWB
	This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determinedby the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions ａnd the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Can also be used for: Immunoprecipitation, Western Blotting: More
Species Reactivity	Human
Concentration	1 mg/ml
Purification	Affinity purified

Target Information

Gene Synonyms	P33993 PNAS146 MCM2 CDC47 P1.1-MCM3 4176 P1CDC47 CDABP0042 600592 P1.1MCM3 MCM7 P85MCM PNAS-146 PNAS 146 P1.1 MCM3 More
Target	CDC47
Cellular Localization	Nuclear

Application

Application

Formalin fixed paraffin embedded human tonsil stained with CDC47 antibody

Positive Control	Breast carcinoma
Application Notes	This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determinedby the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions ａnd the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Can also be used for: Immunoprecipitation, Western Blotting: More

Positive Control

Breast carcinoma

Application Notes

This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determinedby the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions ａnd the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Can also be used for: Immunoprecipitation, Western Blotting:

Form	Liquid
Storage Instructions	Keep as concentrated solution, aliquot ａnd store at 4°C.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

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