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易推广首页>技术文章>产品说明>HLA-G antibody Catalog Number：OM257159

产品说明行业标准分析方法操作维修技术交流技术研讨解决方案

HLA-G antibody Catalog Number：OM257159

发布时间：2024/9/30点击次数：36

Product Profile

Product Name	HLA-G antibody
Antibody Type	Primary Antibodies
Product description	Monoclonal Antibody to HLA-G (Human) Azide freeHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain ａnd a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa ａnd its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 ａnd 3 encode the alpha1 ａnd alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, ａnd exon 6 encodes the cylasmic tail. [provided by RefSeq] More
Immunogen	Recombinant human HLA-G refolded with beta2-microglobulin ａnd peptide.

Key Feature

Clonality	Monoclonal
Isotype	IgG1
Clone Number	MEM-G/9
Host Species	Mouse
Tested Applications	ELISAFACSIHC-FIPWB
	FACS: Recommended dilution: 2-5 ug/ml. Positive control: JEG-3 human choriocarcinoma cell line.ELISA: Coating antibody (10 ug/ml) Detection antibody (biotin or peroxidase conjugate (1 ug/ml):
Species Reactivity	Human
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	Affinity purified

Target Information

Gene Synonyms	142871 HLAG P17693 MHC-G MHCG 3135 HLA-G HLA G MHC G More
Target	HLA-G
Tissue Specificity	The antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).

Application

Application

Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC ａnd anti-human HLA-G (MEM-G/11) FITC.
Application

Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) ａnd protein G. HLA-G was detected by anti-human HLA-G ａnd goat anti-mouse HRP in cell lysate (Lane 1) ａnd in the immunoprecipitate (Lane 2).

Application Notes	FACS: Recommended dilution: 2-5 ug/ml. Positive control: JEG-3 human choriocarcinoma cell line.ELISA: Coating antibody (10 ug/ml) Detection antibody (biotin or peroxidase conjugate (1 ug/ml):

Additional Information

Form	Liquid
Storage Instructions	Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot ａnd store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer	Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4

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