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易推广首页>技术文章>产品说明>HLA-G antibody Catalog Number：OM257160

产品说明行业标准分析方法操作维修技术交流技术研讨解决方案

HLA-G antibody Catalog Number：OM257160

发布时间：2024/9/30点击次数：65

Product Profile

Product Name	HLA-G antibody
Antibody Type	Primary Antibodies
Product description	Mouse monoclonal [MEM - G / 9] to HLA GHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain ａnd a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa ａnd its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 ａnd 3 encode the alpha1 ａnd alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, ａnd exon 6 encodes the cylasmic tail. [provided by RefSeq] More
Immunogen	Recombinant full length protein (Human).

Key Feature

Clonality	Monoclonal
Isotype	IgG1
Clone Number	MEM-G/9
Host Species	Mouse
Tested Applications	ELISAFACSIHC-FWB
	ELISA: Use at an assay dependent concentration. FACS: Use at an assay dependent concentration. IHC-Fr: Use at an assay dependent concentration. Is unsuitable for IHC-P. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity	HumanPrimate
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	Affinity purified

Target Information

Gene Synonyms	HLA G HLAG MHC-G MHC G MHCG More
Target	HLA-G
Tissue Specificity	Reacts with the native form of human HLA-G on the cell surface as well as with soluble HLA-G molecule in its beta 2-microglobulin associated form. Specificity was demonstrated with cells transfected with either full-length HLA-G ａnd HLA-G1 complementary DNA.This antibody does not compete with a tested panel of other monoclonal antibodies to HLA-G.
Cellular Localization	Cell Membrane

Application

Application

Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC ( ) ａnd anti-human HLA-G (MEM-G/11) FITC .
Application

Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) ａnd protein G. HLA-G was detected by anti-human HLA-G (4H84) ａnd goat anti-mouse HRP in cell lysate (Lane 1) ａnd in the immunoprecipitate (Lane 2).

Application Notes	ELISA: Use at an assay dependent concentration. FACS: Use at an assay dependent concentration. IHC-Fr: Use at an assay dependent concentration. Is unsuitable for IHC-P. Optimal dilutions/concentrations should be determined by the end user.:

Additional Information

Form	Liquid
Storage Instructions	Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot ａnd store at -20°C or below. Avoid freeze-thaw cycles.
Storage Buffer	Phosphate buffered saline pH 7.4 containing 15mM sodium azide

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