Fibrinogen gamma antibody Catalog Number：OM253134_产品说明_ B2B电子商务综合技术文献

发布时间：2024/9/19点击次数：7

Product Name	Fibrinogen gamma antibody
Antibody Type	Primary Antibodies
Product description	Mouse monoclonal antibody [4H9] to Fibrinogen gammaThe protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen ａnd fibrin regulate cell adhesion ａnd spreading, display vasoconstrictor ａnd chemotactic activities, ａnd are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia ａnd thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] More
Immunogen	The immunogen corresponding to a region within amino acids 210 ａnd 437 of human FGG.

Clonality	Monoclonal
Isotype	IgG2a
Clone Number	4H9
Host Species	Mouse
Tested Applications	ELISAFACSICC/IFIHC-PWB
	Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. Not yet tested in other applications. Determining optimal working dilutions by titration test:
Species Reactivity	Human
Concentration	1 mg/ml
Purification

Application

WB analysis of human Fibrinogen gamma recombinant protein (aa. 210-437) using Fibrinogen gamma antibody [4H9].
Application

ELISA analysis of control antigen (100ng) ａnd antigen using Fibrinogen gamma antibody [4H9].
Application

FACS analysis of HepG2 cells using Fibrinogen gamma antibody [4H9] (blue) ａnd negative control (red).
Application

ICC/IF analysis of 3T3-L1 cells using Fibrinogen gamma antibody [4H9] (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application

IHC-P analysis of cerebellum tissues using Fibrinogen gamma antibody [4H9].
Application

IHC-P analysis of liver cancer tissues using Fibrinogen gamma antibody [4H9].

Application Notes	Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. Not yet tested in other applications. Determining optimal working dilutions by titration test:

Form	Liquid
Storage Instructions	4°C; -20°C for long term storage
Storage Buffer	Ascitic fluid containing 0.03% sodium azide.

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